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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
20/02/2013 |
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Data da última atualização: |
20/04/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, V. de; FILHO, A. N.; MELO, P. de C.; FERNANDES, G. M.; FERRAUDO, A. S.; CONDE, S. de O.; FOGAÇA JÚNIOR, F. A. |
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Afiliação: |
VIVIANE DE SOUZA, CNPC; Antonio Nader Filho, Universidade Estadual Paulista (UNESP) - Jaboticabal, SP; Poliana de Castro Melo, UNESP - Jaboticabal, SP; Guilherme Moraes Ferraudo, UNESP - Jaboticabal, SP.; Antônio Sérgio Ferraudo, UNESP - Jaboticabal, SP; Sandra de Oliveira Conde, UNESP - Jaboticabal, SP; Flavio Augusto Fogaça Junior, UNESP - Jaboticabal, SP. |
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Título: |
Molecular epidemiology of Staphylococcus aureus isolates at different sites in the milk producing dairy farms. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, São Paulo, v. 43, n. 4, p. 1646-1650, out./dez. 2012. |
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Idioma: |
Português |
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Conteúdo: |
Abstract: The epidemiological relationships between isolated Staphylococcus aureus strains in milk samples of dairy cows, reagent to California Mastitis Test, individual and group milk was demonstrated in different sites of the production fluxogram, in 12 milk-producing farms in the Gameleira region, municipality of Sacramento MG Brazil, so that localization and transmission modes may be identified. Two hundred and forty-four strains out of 446 samples collected at several sites were isolated and bio-chemically characterized as coagulase-positive staphylococcus. Specific chromosome DNA fragment of the species Staphylococcus aureus was amplified to 106 strains and 103 underwent (PFGE). Samples? collection sites with the highest isolation frequency of Staphylococcus aureus strains comprised papillary ostia (31.1%), CMT-reagent cow milk (21.7%), mechanical milking machines? insufflators (21,7%), milk in milk pails (6.6%) and the milk in community bulk tanks (5.6%). Genetic heterogeneity existed among the isolated 103 Staphylococcus aureus strains, since 32 different pulse-types were identified. Pulse-type 1 had the highest similarity among the isolated strains within the different sites of the milk-production fluxogram. Highest occurrence of pulsetype 1 isolates of Staphylococcus aureus strains was reported in samples collected from the papillary ostia (10.6%), followed by milk samples from CMT-reagent dairy cows (5.8%) and mechanical milking machine insufflators (3.8%). The above shows the relevance of these sites in the agents? transmission mechanism within the context of the farms investigated. MenosAbstract: The epidemiological relationships between isolated Staphylococcus aureus strains in milk samples of dairy cows, reagent to California Mastitis Test, individual and group milk was demonstrated in different sites of the production fluxogram, in 12 milk-producing farms in the Gameleira region, municipality of Sacramento MG Brazil, so that localization and transmission modes may be identified. Two hundred and forty-four strains out of 446 samples collected at several sites were isolated and bio-chemically characterized as coagulase-positive staphylococcus. Specific chromosome DNA fragment of the species Staphylococcus aureus was amplified to 106 strains and 103 underwent (PFGE). Samples? collection sites with the highest isolation frequency of Staphylococcus aureus strains comprised papillary ostia (31.1%), CMT-reagent cow milk (21.7%), mechanical milking machines? insufflators (21,7%), milk in milk pails (6.6%) and the milk in community bulk tanks (5.6%). Genetic heterogeneity existed among the isolated 103 Staphylococcus aureus strains, since 32 different pulse-types were identified. Pulse-type 1 had the highest similarity among the isolated strains within the different sites of the milk-production fluxogram. Highest occurrence of pulsetype 1 isolates of Staphylococcus aureus strains was reported in samples collected from the papillary ostia (10.6%), followed by milk samples from CMT-reagent dairy cows (5.8%) and mechanical milking machine insufflators (3.8%). The ab... Mostrar Tudo |
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Palavras-Chave: |
Brasil; Bulk tank; Minas Gerais; PCR; PFGE. |
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Thesagro: |
Doença animal; Mamite; Staphylococcus aureus; Vaca leiteira. |
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Thesaurus Nal: |
Brazil; Dairy cows; Mammary gland diseases; Mastitis. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/76787/1/API-Molecular-epidemiology.pdf.pdf
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Marc: |
LEADER 02617naa a2200349 a 4500
001 1950132
005 2015-04-20
008 2012 bl uuuu u00u1 u #d
100 1 $aSOUZA, V. de
245 $aMolecular epidemiology of Staphylococcus aureus isolates at different sites in the milk producing dairy farms.$h[electronic resource]
260 $c2012
520 $aAbstract: The epidemiological relationships between isolated Staphylococcus aureus strains in milk samples of dairy cows, reagent to California Mastitis Test, individual and group milk was demonstrated in different sites of the production fluxogram, in 12 milk-producing farms in the Gameleira region, municipality of Sacramento MG Brazil, so that localization and transmission modes may be identified. Two hundred and forty-four strains out of 446 samples collected at several sites were isolated and bio-chemically characterized as coagulase-positive staphylococcus. Specific chromosome DNA fragment of the species Staphylococcus aureus was amplified to 106 strains and 103 underwent (PFGE). Samples? collection sites with the highest isolation frequency of Staphylococcus aureus strains comprised papillary ostia (31.1%), CMT-reagent cow milk (21.7%), mechanical milking machines? insufflators (21,7%), milk in milk pails (6.6%) and the milk in community bulk tanks (5.6%). Genetic heterogeneity existed among the isolated 103 Staphylococcus aureus strains, since 32 different pulse-types were identified. Pulse-type 1 had the highest similarity among the isolated strains within the different sites of the milk-production fluxogram. Highest occurrence of pulsetype 1 isolates of Staphylococcus aureus strains was reported in samples collected from the papillary ostia (10.6%), followed by milk samples from CMT-reagent dairy cows (5.8%) and mechanical milking machine insufflators (3.8%). The above shows the relevance of these sites in the agents? transmission mechanism within the context of the farms investigated.
650 $aBrazil
650 $aDairy cows
650 $aMammary gland diseases
650 $aMastitis
650 $aDoença animal
650 $aMamite
650 $aStaphylococcus aureus
650 $aVaca leiteira
653 $aBrasil
653 $aBulk tank
653 $aMinas Gerais
653 $aPCR
653 $aPFGE
700 1 $aFILHO, A. N.
700 1 $aMELO, P. de C.
700 1 $aFERNANDES, G. M.
700 1 $aFERRAUDO, A. S.
700 1 $aCONDE, S. de O.
700 1 $aFOGAÇA JÚNIOR, F. A.
773 $tBrazilian Journal of Microbiology, São Paulo$gv. 43, n. 4, p. 1646-1650, out./dez. 2012.
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Embrapa Caprinos e Ovinos (CNPC) |
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| 1. |  | SOUZA, V. de; FILHO, A. N.; MELO, P. de C.; FERNANDES, G. M.; FERRAUDO, A. S.; CONDE, S. de O.; FOGAÇA JÚNIOR, F. A. Molecular epidemiology of Staphylococcus aureus isolates at different sites in the milk producing dairy farms. Brazilian Journal of Microbiology, São Paulo, v. 43, n. 4, p. 1646-1650, out./dez. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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