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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
21/12/2011 |
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Data da última atualização: |
08/08/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARDOSO, J. D.; HUNGRIA, M.; ANDRADE, D. S. |
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Afiliação: |
JUSCÉLIO DONIZETE CARDOSO, UEL; MARIANGELA HUNGRIA DA CUNHA, CNPSO; DIVA S. ANDRADE, IAPAR. |
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Título: |
Polyphasic approach for the characterization of rhizobial symbionts effective in fixing N2 with common bean (Phaseolus vulgaris L.). |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Applied Microbiology and Biotechnology, Heidelberg, v. 93, n. 5, p. 2035-2049, mar. 2012. |
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DOI: |
10.1007/s00253-011-3708-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Common bean (Phaseolus vulgaris L.) is a legume that has been reported as highly promiscuous in nodulating with a variety of rhizobial strains, often with
low effectiveness in fixing nitrogen. The aim of this work was to assess the symbiotic efficiency of rhizobial strains isolated from common bean seeds, nodules of Arachis hypogaea, Mucuna pruriens, and soils from various Brazilian agroecosystems, followed by the characterization of elite strains identified in the first screening. Forty-five elite strains were analyzed for symbiotic properties (nodulation, plant-growth, and nitrogen-fixation parameters) under greenhouse conditions in pots containing non-sterile soil, and variation in symbiotic performance was observed. Elite strains were also characterized in relation to morphophysiological properties, genetic profiles of reppolymerase chain reaction (PCR; BOX), and restriction fragment length polymorphism (RFLP)-PCR of the 16S
rRNA. Sequence analyses of the 16S rRNA were obtained for 17 strains representative of the main groups resulting from all previous analyses. One of the most effective strains, IPR-Pv 2604, was clustered with Rhizobium tropici, whereas strain IPR-Pv 583, showing lower effectiveness in fixing N2, was clustered with Herbaspirillum lusitanum. Surprisingly, effective strains were clustered with unusual symbiotic genera/species, including Leifsonia xyli, Stenotrophomonas maltophilia, Burkholderia, and Enterobacter. Some strains recognized in this study were outstanding in their nitrogen-fixing capacity and therefore, show high biotechnological potential for use in commercial inoculants. MenosCommon bean (Phaseolus vulgaris L.) is a legume that has been reported as highly promiscuous in nodulating with a variety of rhizobial strains, often with
low effectiveness in fixing nitrogen. The aim of this work was to assess the symbiotic efficiency of rhizobial strains isolated from common bean seeds, nodules of Arachis hypogaea, Mucuna pruriens, and soils from various Brazilian agroecosystems, followed by the characterization of elite strains identified in the first screening. Forty-five elite strains were analyzed for symbiotic properties (nodulation, plant-growth, and nitrogen-fixation parameters) under greenhouse conditions in pots containing non-sterile soil, and variation in symbiotic performance was observed. Elite strains were also characterized in relation to morphophysiological properties, genetic profiles of reppolymerase chain reaction (PCR; BOX), and restriction fragment length polymorphism (RFLP)-PCR of the 16S
rRNA. Sequence analyses of the 16S rRNA were obtained for 17 strains representative of the main groups resulting from all previous analyses. One of the most effective strains, IPR-Pv 2604, was clustered with Rhizobium tropici, whereas strain IPR-Pv 583, showing lower effectiveness in fixing N2, was clustered with Herbaspirillum lusitanum. Surprisingly, effective strains were clustered with unusual symbiotic genera/species, including Leifsonia xyli, Stenotrophomonas maltophilia, Burkholderia, and Enterobacter. Some strains recognized in this study wer... Mostrar Tudo |
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Palavras-Chave: |
Fixação biológica de nitrogênio. |
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Thesagro: |
Feijão; Fixação de Nitrogênio. |
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Thesaurus Nal: |
Beans; Nitrogen fixation. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02357naa a2200217 a 4500
001 1910587
005 2018-08-08
008 2012 bl --- 0-- u #d
024 7 $a10.1007/s00253-011-3708-2$2DOI
100 1 $aCARDOSO, J. D.
245 $aPolyphasic approach for the characterization of rhizobial symbionts effective in fixing N2 with common bean (Phaseolus vulgaris L.).
260 $c2012
520 $aCommon bean (Phaseolus vulgaris L.) is a legume that has been reported as highly promiscuous in nodulating with a variety of rhizobial strains, often with low effectiveness in fixing nitrogen. The aim of this work was to assess the symbiotic efficiency of rhizobial strains isolated from common bean seeds, nodules of Arachis hypogaea, Mucuna pruriens, and soils from various Brazilian agroecosystems, followed by the characterization of elite strains identified in the first screening. Forty-five elite strains were analyzed for symbiotic properties (nodulation, plant-growth, and nitrogen-fixation parameters) under greenhouse conditions in pots containing non-sterile soil, and variation in symbiotic performance was observed. Elite strains were also characterized in relation to morphophysiological properties, genetic profiles of reppolymerase chain reaction (PCR; BOX), and restriction fragment length polymorphism (RFLP)-PCR of the 16S rRNA. Sequence analyses of the 16S rRNA were obtained for 17 strains representative of the main groups resulting from all previous analyses. One of the most effective strains, IPR-Pv 2604, was clustered with Rhizobium tropici, whereas strain IPR-Pv 583, showing lower effectiveness in fixing N2, was clustered with Herbaspirillum lusitanum. Surprisingly, effective strains were clustered with unusual symbiotic genera/species, including Leifsonia xyli, Stenotrophomonas maltophilia, Burkholderia, and Enterobacter. Some strains recognized in this study were outstanding in their nitrogen-fixing capacity and therefore, show high biotechnological potential for use in commercial inoculants.
650 $aBeans
650 $aNitrogen fixation
650 $aFeijão
650 $aFixação de Nitrogênio
653 $aFixação biológica de nitrogênio
700 1 $aHUNGRIA, M.
700 1 $aANDRADE, D. S.
773 $tApplied Microbiology and Biotechnology, Heidelberg$gv. 93, n. 5, p. 2035-2049, mar. 2012.
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Embrapa Soja (CNPSO) |
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| Registros recuperados : 52 | |
| 2. |  | ANDRADE, D. S.; HUNGRIA, M. Maximizing the contribution of biological nitrogen fixation in tropical legume crops. In: INTERNATIONAL CONGRESS ON NITROGEN FIXATION, 13., 2001, Ontario. Nitrogen fixation: global perspectives: proceedings. [S.l.]: CABI Publishing, 2002. p. 341-345. Edited by T.M. Finan, M.R. O'Brian, D.B. Layzell, J.K. Vessey, W. Newton.| Biblioteca(s): Embrapa Soja. |
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| 4. | | KASCHUK, G.; HUNGRIA, M.; ANDRADE, D. S. Diversidade de rizóbios que nodulam o feijoeiro (phaseolus vulgaris L.) sob os sistemas de plantios direto e convencional em Ponta grossa, PR. In: WORLD CONGRESS ON CONSERVATION AGRICULTURE = CONGRESSO MUNDIAL SOBRE AGRICULTURA CONSERVACIONISTA, 2., 2003, Foz do Iguaçu. Producing in harmony with nature: [proceedings ...] = Produzindo em harmonia com a natureza: [anais ...]. Ponta Grossa: Federação Brasileira de Plantio Direto na Palha, 2003. v. 2 p. 452-455. II World Congress on Conservation Agriculture, Iguassu Falls, 2003. Extended summary / posters.| Biblioteca(s): Embrapa Soja. |
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| 9. | | HUNGRIA, M.; VARGAS, M. A. T.; CAMPO, R. J.; CHUEIRE, L. M. O.; ANDRADE, D. S. The Brazilian experience with the soybean (Glycine max) and common bean (Phaseolus vulgaris) symbioses. In: INTERNATIONAL CONGRESS ON NITROGEN FIXATION, 12., 1999, Foz do Iguacu. Nitrogen fixation: from molecules to crop productivity: proceedings. Dordrechet: Kluwer, 2000. p. 515-518. (Current Plant Science and Biotechnology in Agriculture, 38). Editado por Fabio O. Pedrosa, Mariangela Hungria, Geoffrey Yates, William E. Newton.| Biblioteca(s): Embrapa Soja. |
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| 13. | | BALOTA, E. L.; COLOZZI-FILHO, A.; ANDRADE, D. S.; HUNGRIA, M. Biomassa microbiana e sua atividade em solos sob diferentes sistemas de preparo e sucessão de culturas. Revista Brasileira de Ciência do Solo, Viçosa, MG, v. 22, n. 4, p. 641-649, Oct./Dec. 1998.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
| Biblioteca(s): Embrapa Soja. |
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| 14. | | HUNGRIA, M.; ANDRADE, D. S.; COLOZZO-FILHO, A.; BALOTA, E. L.; CATTELAN, A. J. Ecologia microbiana em solos sob cultivo na região sul do Brasil. In: SIMPÓSIO BRASILEIRO SOBRE MICROBIOLOGIA DO SOLO, 3.; REUNIÃO DE LABORATÓRIOS PARA RECOMENDAÇÃO DE ESTIRPES DE RHIZOBIUM E BRADYRHIZOBIUM, 6., 1994, Londrina. Resumos... Londrina: IAPAR, 1994. Não paginado. Trabalho n. 22.| Biblioteca(s): Embrapa Soja. |
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| 15. | | GOMES, D. F.; CARDOSO, J. C.; MATOS, M. A.; HUNGRIA, M.; ANDRADE, D. S. Effect of liming on plasmid profile of Rhizobium strains. In: INTERNATIONAL INCT SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 1., 2009, Curitiba. Program and index... Curitiba: INCT, 2009. Conference , P31.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 17. | | GOMES, D. F.; CARDOSO, J. D.; MATOS, M. A.; ANDRADE, D. S.; HUNGRIA, M. Efeito da calagem e fosfatagem na diversidade de rhizobium-phaseolus em solo ácido. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 25., 2009, Porto de Galinhas. Anais... [São Paulo]: Sociedade Brasileira de Microbiologia, 2009. Seção Resumos, ref. 2364-2. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 20. | | ANDRADE, D. S.; COLOZZI-FILHO, A.; HUNGRIA, M.; MATOS, A. M.; MACHINESKI, O. População de rizóbios e nodulação do feijoeiro em cultivo agro-ecológico. In: WORLD CONGRESS ON CONSERVATION AGRICULTURE = CONGRESSO MUNDIAL SOBRE AGRICULTURA CONSERVACIONISTA, 2., 2003, Foz do Iguaçu. Producing in harmony with nature: [proceedings ...] = Produzindo em harmonia com a natureza: [anais ...]. Ponta Grossa: Federação Brasileira de Plantio Direto na Palha, 2003. v. 2 p. 448-451. II World Congress on Conservation Agriculture, Iguassu Falls, 2003. Extended summary / posters.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 52 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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