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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
21/12/2011 |
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Data da última atualização: |
26/03/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARDOSO, J. D.; HUNGRIA, M.; ANDRADE, D. S. |
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Afiliação: |
JUSCÉLIO DONIZETE CARDOSO, UEL; MARIANGELA HUNGRIA DA CUNHA, CNPSO; DIVA S. ANDRADE, IAPAR. |
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Título: |
Polyphasic approach for the characterization of rhizobial symbionts effective in fixing N2 with common bean (Phaseolus vulgaris L.). |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Applied Microbiology and Biotechnology, v. 93, n. 5, p. 2035-2049, mar. 2012. |
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DOI: |
10.1007/s00253-011-3708-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Common bean (Phaseolus vulgaris L.) is a legume that has been reported as highly promiscuous in nodulating with a variety of rhizobial strains, often with low effectiveness in fixing nitrogen. The aim of this work was to assess the symbiotic efficiency of rhizobial strains isolated from common bean seeds, nodules of Arachis hypogaea, Mucuna pruriens, and soils from various Brazilian agroecosystems, followed by the characterization of elite strains identified in the first screening. Forty-five elite strains were analyzed for symbiotic properties (nodulation, plant-growth, and nitrogen-fixation parameters) under greenhouse conditions in pots containing non-sterile soil, and variation in symbiotic performance was observed. Elite strains were also characterized in relation to morphophysiological properties, genetic profiles of reppolymerase chain reaction (PCR; BOX), and restriction fragment length polymorphism (RFLP)-PCR of the 16S rRNA. Sequence analyses of the 16S rRNA were obtained for 17 strains representative of the main groups resulting from all previous analyses. One of the most effective strains, IPR-Pv 2604, was clustered with Rhizobium tropici, whereas strain IPR-Pv 583, showing lower effectiveness in fixing N2, was clustered with Herbaspirillum lusitanum. Surprisingly, effective strains were clustered with unusual symbiotic genera/species, including Leifsonia xyli, Stenotrophomonas maltophilia, Burkholderia, and Enterobacter. Some strains recognized in this study were outstanding in their nitrogen-fixing capacity and therefore, show high biotechnological potential for use in commercial inoculants. MenosCommon bean (Phaseolus vulgaris L.) is a legume that has been reported as highly promiscuous in nodulating with a variety of rhizobial strains, often with low effectiveness in fixing nitrogen. The aim of this work was to assess the symbiotic efficiency of rhizobial strains isolated from common bean seeds, nodules of Arachis hypogaea, Mucuna pruriens, and soils from various Brazilian agroecosystems, followed by the characterization of elite strains identified in the first screening. Forty-five elite strains were analyzed for symbiotic properties (nodulation, plant-growth, and nitrogen-fixation parameters) under greenhouse conditions in pots containing non-sterile soil, and variation in symbiotic performance was observed. Elite strains were also characterized in relation to morphophysiological properties, genetic profiles of reppolymerase chain reaction (PCR; BOX), and restriction fragment length polymorphism (RFLP)-PCR of the 16S rRNA. Sequence analyses of the 16S rRNA were obtained for 17 strains representative of the main groups resulting from all previous analyses. One of the most effective strains, IPR-Pv 2604, was clustered with Rhizobium tropici, whereas strain IPR-Pv 583, showing lower effectiveness in fixing N2, was clustered with Herbaspirillum lusitanum. Surprisingly, effective strains were clustered with unusual symbiotic genera/species, including Leifsonia xyli, Stenotrophomonas maltophilia, Burkholderia, and Enterobacter. Some strains recognized in this study wer... Mostrar Tudo |
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Palavras-Chave: |
Fixação biológica de nitrogênio. |
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Thesagro: |
Feijão; Fixação de Nitrogênio. |
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Thesaurus Nal: |
Beans; Nitrogen fixation. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02368naa a2200217 a 4500
001 1910587
005 2025-03-26
008 2012 bl uuuu u00u1 u #d
024 7 $a10.1007/s00253-011-3708-2$2DOI
100 1 $aCARDOSO, J. D.
245 $aPolyphasic approach for the characterization of rhizobial symbionts effective in fixing N2 with common bean (Phaseolus vulgaris L.).$h[electronic resource]
260 $c2012
520 $aCommon bean (Phaseolus vulgaris L.) is a legume that has been reported as highly promiscuous in nodulating with a variety of rhizobial strains, often with low effectiveness in fixing nitrogen. The aim of this work was to assess the symbiotic efficiency of rhizobial strains isolated from common bean seeds, nodules of Arachis hypogaea, Mucuna pruriens, and soils from various Brazilian agroecosystems, followed by the characterization of elite strains identified in the first screening. Forty-five elite strains were analyzed for symbiotic properties (nodulation, plant-growth, and nitrogen-fixation parameters) under greenhouse conditions in pots containing non-sterile soil, and variation in symbiotic performance was observed. Elite strains were also characterized in relation to morphophysiological properties, genetic profiles of reppolymerase chain reaction (PCR; BOX), and restriction fragment length polymorphism (RFLP)-PCR of the 16S rRNA. Sequence analyses of the 16S rRNA were obtained for 17 strains representative of the main groups resulting from all previous analyses. One of the most effective strains, IPR-Pv 2604, was clustered with Rhizobium tropici, whereas strain IPR-Pv 583, showing lower effectiveness in fixing N2, was clustered with Herbaspirillum lusitanum. Surprisingly, effective strains were clustered with unusual symbiotic genera/species, including Leifsonia xyli, Stenotrophomonas maltophilia, Burkholderia, and Enterobacter. Some strains recognized in this study were outstanding in their nitrogen-fixing capacity and therefore, show high biotechnological potential for use in commercial inoculants.
650 $aBeans
650 $aNitrogen fixation
650 $aFeijão
650 $aFixação de Nitrogênio
653 $aFixação biológica de nitrogênio
700 1 $aHUNGRIA, M.
700 1 $aANDRADE, D. S.
773 $tApplied Microbiology and Biotechnology$gv. 93, n. 5, p. 2035-2049, mar. 2012.
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Registro original: |
Embrapa Soja (CNPSO) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
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URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
18/02/1999 |
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Data da última atualização: |
12/03/2025 |
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Autoria: |
SOUZA, M. L. de; CASTRO, M. E. B. de; SIHLER, W. NAKAMURA, E. S. |
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Título: |
Identificacao e caracterizacao de isolados de baculovirus com potencial para controle biológico de Spodoptera frugiperda. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Brasilia: Embrapa-Cenargen, 1998 |
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Páginas: |
7 p. |
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Série: |
(Embrapa-Cenargen. Comunicado Tecnico, 26) |
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Idioma: |
Português |
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Conteúdo: |
Varios isolados pertencentes aos generos Nucleopolyhedrovirus e Granulovirus, com potencial para uso no contrle da lagarta do cartucho do milho (Sodoptera frugiperda) foram identificados e caracterizados. Os isolados encontram-se armazenados a -20o (freezer), para posteriores estudos de caracterizacao incluindo analise de DNA viral e testes de patogenicidade. |
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Palavras-Chave: |
Brasil; Brasilia; Caracterizacao; Ganulovirus; Genero; Granulovirus; Identification; Isolado; Lagarta do cartucho; Lagarta-do-cartucho; Maise; Maize; Mayse; Nucleopolyhedrovirus; Pests of plants; Spodoptera fugiperda. |
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Thesagro: |
Baculovirus; Controle Biológico; Identificação; Lagarta; Milho; Planta; Praga; Praga de Planta; Spodoptera Frugiperda; Zea Mays. |
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Thesaurus NAL: |
Baculoviridae; biological control; larvae; pests. |
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Categoria do assunto: |
-- |
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URL: |
https://www.infoteca.cnptia.embrapa.br/infoteca/bitstream/doc/174567/1/1737.pdf
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Marc: |
LEADER 01729nam a2200517 a 4500
001 1174567
005 2025-03-12
008 1998 bl uuuu u0uu1 u #d
100 1 $aSOUZA, M. L. de
245 $aIdentificacao e caracterizacao de isolados de baculovirus com potencial para controle biológico de Spodoptera frugiperda.
260 $aBrasilia: Embrapa-Cenargen$c1998
300 $a7 p.
490 $a(Embrapa-Cenargen. Comunicado Tecnico, 26)
520 $aVarios isolados pertencentes aos generos Nucleopolyhedrovirus e Granulovirus, com potencial para uso no contrle da lagarta do cartucho do milho (Sodoptera frugiperda) foram identificados e caracterizados. Os isolados encontram-se armazenados a -20o (freezer), para posteriores estudos de caracterizacao incluindo analise de DNA viral e testes de patogenicidade.
650 $aBaculoviridae
650 $abiological control
650 $alarvae
650 $apests
650 $aBaculovirus
650 $aControle Biológico
650 $aIdentificação
650 $aLagarta
650 $aMilho
650 $aPlanta
650 $aPraga
650 $aPraga de Planta
650 $aSpodoptera Frugiperda
650 $aZea Mays
653 $aBrasil
653 $aBrasilia
653 $aCaracterizacao
653 $aGanulovirus
653 $aGenero
653 $aGranulovirus
653 $aIdentification
653 $aIsolado
653 $aLagarta do cartucho
653 $aLagarta-do-cartucho
653 $aMaise
653 $aMaize
653 $aMayse
653 $aNucleopolyhedrovirus
653 $aPests of plants
653 $aSpodoptera fugiperda
700 1 $aCASTRO, M. E. B. de
700 1 $aSIHLER, W. NAKAMURA, E. S.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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