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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Suínos e Aves. |
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Data corrente: |
19/12/2011 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NASCIMENTO, C. S.; MACHADO, M. A.; GUIMARÃES, S. E. F.; MARTINS, M. F.; PEIXOTO, J. de O.; FURLONG, J.; PRATA, M. C. de A.; VERNEQUE, R. da S.; TEODORO, R. L.; LOPES, P. S. |
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Afiliação: |
C. S. NASCIMENTO, UFV; MARCO ANTONIO MACHADO, CNPGL; S. E. F. GUIMARÃES, UFV; MARTA FONSECA MARTINS, CNPGL; JANE DE OLIVEIRA PEIXOTO, CNPSA; JOHN FURLONG, CNPGL; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL; RUI DA SILVA VERNEQUE, CNPGL; ROBERTO LUIZ TEODORO, Pesquisador aposentado do CNPGL; P. S. LOPES, UFV. |
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Título: |
Expressed sequenced tags profiling of resistant and susceptible Gyr x Holstein cattle infested with the tick Rhipicephalus (Boophilus) microplus. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 10, n. 4, 2011. |
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Páginas: |
14 p. |
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DOI: |
https://doi.org/10.4238/2011.november.8.3 |
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Idioma: |
Inglês |
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Conteúdo: |
Tick resistance in cattle is mainly found in zebu (Bos indicus) animals, although it is also present in some taurine (B. taurus) breeds. In order to characterize functional genes involved in tick resistance/susceptibility in cattle, two cDNA libraries were generated using skin tissues of selected Holstein x Gyr animals. A total of 2700 high-quality reads from both resistant and susceptible cDNA were assembled into 458 sequences (contigs) and 834 singletons, with a mean size of 447.7 nucleotides. Assignment of homologous proteins by BLASTX revealed 790 (61.1%) and 300 (23.2%) hits in resistant and susceptible cDNA, respectively; 121 of these hits matched bovine proteins. A total of 502 (38.9%) unique sequences were found to have no significant homology with known sequences and were classified as novel sequences. In general, the most abundant sequences consisted of those coding for hypothetical proteins whose function had not yet been determined, in addition to ribosomal proteins, binding proteins and structural proteins, such as keratin and collagen. The most abundant protein found was collagen type III alpha, although ribosomal proteins accounted for half of the 40 most frequent hits. In addition, five matches within the top 40 best hits corresponded to immune response proteins. These sequences could be used for future studies on functional genomics of cattle tick resistance as well as for genomic sequencing projects. |
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Palavras-Chave: |
Bovine; CDNA library; Crossbreeds; Skin. |
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Thesagro: |
Bovino; Genética. |
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Thesaurus Nal: |
expressed sequence tags. |
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Categoria do assunto: |
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L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/50834/1/Artigo-meta-2011-gmr1506-Marco-Antonio.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/56217/1/EXPRESSED-SEQUENCED.pdf
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Marc: |
LEADER 02454naa a2200337 a 4500
001 1910262
005 2024-02-05
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.4238/2011.november.8.3$2DOI
100 1 $aNASCIMENTO, C. S.
245 $aExpressed sequenced tags profiling of resistant and susceptible Gyr x Holstein cattle infested with the tick Rhipicephalus (Boophilus) microplus.$h[electronic resource]
260 $c2011
300 $a14 p.
520 $aTick resistance in cattle is mainly found in zebu (Bos indicus) animals, although it is also present in some taurine (B. taurus) breeds. In order to characterize functional genes involved in tick resistance/susceptibility in cattle, two cDNA libraries were generated using skin tissues of selected Holstein x Gyr animals. A total of 2700 high-quality reads from both resistant and susceptible cDNA were assembled into 458 sequences (contigs) and 834 singletons, with a mean size of 447.7 nucleotides. Assignment of homologous proteins by BLASTX revealed 790 (61.1%) and 300 (23.2%) hits in resistant and susceptible cDNA, respectively; 121 of these hits matched bovine proteins. A total of 502 (38.9%) unique sequences were found to have no significant homology with known sequences and were classified as novel sequences. In general, the most abundant sequences consisted of those coding for hypothetical proteins whose function had not yet been determined, in addition to ribosomal proteins, binding proteins and structural proteins, such as keratin and collagen. The most abundant protein found was collagen type III alpha, although ribosomal proteins accounted for half of the 40 most frequent hits. In addition, five matches within the top 40 best hits corresponded to immune response proteins. These sequences could be used for future studies on functional genomics of cattle tick resistance as well as for genomic sequencing projects.
650 $aexpressed sequence tags
650 $aBovino
650 $aGenética
653 $aBovine
653 $aCDNA library
653 $aCrossbreeds
653 $aSkin
700 1 $aMACHADO, M. A.
700 1 $aGUIMARÃES, S. E. F.
700 1 $aMARTINS, M. F.
700 1 $aPEIXOTO, J. de O.
700 1 $aFURLONG, J.
700 1 $aPRATA, M. C. de A.
700 1 $aVERNEQUE, R. da S.
700 1 $aTEODORO, R. L.
700 1 $aLOPES, P. S.
773 $tGenetics and Molecular Research$gv. 10, n. 4, 2011.
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Embrapa Gado de Leite (CNPGL) |
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