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Registro Completo |
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Biblioteca(s): |
Embrapa Soja; Embrapa Unidades Centrais. |
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Data corrente: |
24/03/2011 |
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Data da última atualização: |
14/10/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
STOLF-MOREIRA, R.; LEMOS, E. G. de M.; ABDELNOOR, R. V.; BENEVENTI, M. A.; ROLLA, A. A. P.; PEREIRA, S. dos S.; OLIVEIRA, M. C. N. de; NEPOMUCENO, A. L.; MARCELINO-GUIMARÃES, F. C. |
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Afiliação: |
RENATA STOLF-MOREIRA, UNESP Jaboticabal; ELIANA GERTRUDES DE MACEDO LEMOS, UNESP Jaboticabal; RICARDO VILELA ABDELNOOR, CNPSO; MAGDA APARECIDA BENEVENTI, UFRGS; AMANDA ALVES PAIVA ROLLA, UEL; SELMA DOS SANTOS PEREIRA, UEL; MARIA CRISTINA NEVES DE OLIVEIRA, CNPSO; ALEXANDRE LIMA NEPOMUCENO, CNPSO; FRANCISMAR CORREA MARCELINO, CNPSO. |
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Título: |
Identification of reference genes for expression analysis by real-time quantitative PCR in drought-stressed soybean. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 46, n. 1, p. 58-65, jan. 2011. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this work was to validate, by quantitative PCR in real time (RT-qPCR), genes to be used as reference in studies of gene expression in soybean in drought-stressed trials. Four genes commonly used in soybean were evaluated: GmB-actin, GmGAPDH, GmLectin and GmRNAr18S. Total RNA was extracted from six samples: three from roots in a hydroponic system with different drought intensities (0, 25, 50, 75 and 100 minutes of water stress), and three from leaves of plants grown in sand with different soil moistures (15, 5 and 2.5% gravimetric humidity). The raw cycle threshold (Ct) data were analyzed, and the efficiency of each primer was calculated for an overall analysis of the Ct range among the different samples. The GeNorm application was used to evaluate the best reference gene, according to its stability. The GmGAPDH was the least stable gene, with the highest mean values of expression stability (M), and the most stable genes, with the lowest M values, were the GmB-actin and GmRNAr18S, when both root and leaves samples were tested. These genes can be used in RT-qPCR as reference gene for expression analysis. |
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Palavras-Chave: |
Déficit hídrico; Estabilidade de expressão; Expressão gênica; Expression stability; RT-qPCR. |
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Thesagro: |
Biotecnologia; Deficiência hídrica; Glycine Max; Soja. |
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Thesaurus Nal: |
Agricultural biotechnology; Gene expression; Plant-water relations; Soybeans. |
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Categoria do assunto: |
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F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/31023/1/46n01a08.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/168638/1/Identification-of-reference-genes-for-expression.pdf
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Marc: |
LEADER 02275naa a2200373 a 4500
001 1882641
005 2011-10-14
008 2011 bl uuuu u00u1 u #d
100 1 $aSTOLF-MOREIRA, R.
245 $aIdentification of reference genes for expression analysis by real-time quantitative PCR in drought-stressed soybean.
260 $c2011
520 $aThe objective of this work was to validate, by quantitative PCR in real time (RT-qPCR), genes to be used as reference in studies of gene expression in soybean in drought-stressed trials. Four genes commonly used in soybean were evaluated: GmB-actin, GmGAPDH, GmLectin and GmRNAr18S. Total RNA was extracted from six samples: three from roots in a hydroponic system with different drought intensities (0, 25, 50, 75 and 100 minutes of water stress), and three from leaves of plants grown in sand with different soil moistures (15, 5 and 2.5% gravimetric humidity). The raw cycle threshold (Ct) data were analyzed, and the efficiency of each primer was calculated for an overall analysis of the Ct range among the different samples. The GeNorm application was used to evaluate the best reference gene, according to its stability. The GmGAPDH was the least stable gene, with the highest mean values of expression stability (M), and the most stable genes, with the lowest M values, were the GmB-actin and GmRNAr18S, when both root and leaves samples were tested. These genes can be used in RT-qPCR as reference gene for expression analysis.
650 $aAgricultural biotechnology
650 $aGene expression
650 $aPlant-water relations
650 $aSoybeans
650 $aBiotecnologia
650 $aDeficiência hídrica
650 $aGlycine Max
650 $aSoja
653 $aDéficit hídrico
653 $aEstabilidade de expressão
653 $aExpressão gênica
653 $aExpression stability
653 $aRT-qPCR
700 1 $aLEMOS, E. G. de M.
700 1 $aABDELNOOR, R. V.
700 1 $aBENEVENTI, M. A.
700 1 $aROLLA, A. A. P.
700 1 $aPEREIRA, S. dos S.
700 1 $aOLIVEIRA, M. C. N. de
700 1 $aNEPOMUCENO, A. L.
700 1 $aMARCELINO-GUIMARÃES, F. C.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 46, n. 1, p. 58-65, jan. 2011.
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Registro original: |
Embrapa Soja (CNPSO) |
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| 1. |  | STOLF-MOREIRA, R.; LEMOS, E. G. de M.; ABDELNOOR, R. V.; BENEVENTI, M. A.; ROLLA, A. A. P.; PEREIRA, S. dos S.; OLIVEIRA, M. C. N. de; NEPOMUCENO, A. L.; MARCELINO-GUIMARÃES, F. C. Identification of reference genes for expression analysis by real-time quantitative PCR in drought-stressed soybean. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 46, n. 1, p. 58-65, jan. 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Soja; Embrapa Unidades Centrais. |
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| Registros recuperados : 1 | |
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