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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
22/03/2011 |
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Data da última atualização: |
05/06/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
COSTA, F. M.; BASTIANEL, M.; FREITAS-ASTUA, J.; KUBO, K. S.; MACHADO, M. A.; GONDIM JUNIOR, M. G. C. |
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Afiliação: |
F. M. Costa, UFRPE; Marinês Bastianel, APTA; JULIANA DE FREITAS ASTUA, CNPMF; Karen Sumire Kubo, IAC; Marcos Antonio Machado, APTA; Manoel Guedes Corrêa Gondim Júnior, APTA. |
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Título: |
Detection of coffee ringspot virus (CoRSV) in the mite vector by RT-qPCR. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In:INTERNATIONAL CONGRESS OF ACAROLOGY, 13., 2010, Recife. Abstracts book... Recife: CNPq: FACEPE: Banco do Nordeste: TWAS, 2010. p. 61-62. |
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Idioma: |
Inglês |
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Notas: |
Abstract 109 |
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Conteúdo: |
The mite Brevipalpus phoenicis (Geijskes, 1939) (Acari: Tenuipalpidae) is responsible for the transmission of coffee ringspot. There is a great interest in the study of Brevipalpus transmitted viruses (BrTVs), and the interaction between them, their vectors and hosts. Electron microscopy analyses suggest that CoRSV replicates in the mite tissue, what would characterize a persistent propagative virus-vector relationship. This study aimed to establish a transcriptase quantitative polymerase chain reaction (RT-qPCR) method to evaluate and compare the replication capacity of the virus in the vector. Total RNA was extracted from a sample of 100 mites reared in symptomatic plants for CoRSV using two different methods: CTAB and Nucleo Spin RNA XS Kit (Macherey-Nagel) and quantified in a NanoDrop 8000 (Thermo Scientific). |
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Thesagro: |
Brevipalpus Phoenicis; Vírus. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/31193/1/13CongASTUAID27574.pdf
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Marc: |
LEADER 01521nam a2200205 a 4500
001 1881980
005 2023-06-05
008 2010 bl uuuu u00u1 u #d
100 1 $aCOSTA, F. M.
245 $aDetection of coffee ringspot virus (CoRSV) in the mite vector by RT-qPCR.$h[electronic resource]
260 $aIn:INTERNATIONAL CONGRESS OF ACAROLOGY, 13., 2010, Recife. Abstracts book... Recife: CNPq: FACEPE: Banco do Nordeste: TWAS, 2010. p. 61-62.$c2010
500 $aAbstract 109
520 $aThe mite Brevipalpus phoenicis (Geijskes, 1939) (Acari: Tenuipalpidae) is responsible for the transmission of coffee ringspot. There is a great interest in the study of Brevipalpus transmitted viruses (BrTVs), and the interaction between them, their vectors and hosts. Electron microscopy analyses suggest that CoRSV replicates in the mite tissue, what would characterize a persistent propagative virus-vector relationship. This study aimed to establish a transcriptase quantitative polymerase chain reaction (RT-qPCR) method to evaluate and compare the replication capacity of the virus in the vector. Total RNA was extracted from a sample of 100 mites reared in symptomatic plants for CoRSV using two different methods: CTAB and Nucleo Spin RNA XS Kit (Macherey-Nagel) and quantified in a NanoDrop 8000 (Thermo Scientific).
650 $aBrevipalpus Phoenicis
650 $aVírus
700 1 $aBASTIANEL, M.
700 1 $aFREITAS-ASTUA, J.
700 1 $aKUBO, K. S.
700 1 $aMACHADO, M. A.
700 1 $aGONDIM JUNIOR, M. G. C.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Amapá; Embrapa Amazônia Ocidental. |
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Data corrente: |
16/05/2013 |
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Data da última atualização: |
26/01/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
DUTRA, V. S.; RONCHI-TELES, B.; GARCIA, M. V. B.; ADAIME, R.; SILVA, J. G. |
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Afiliação: |
Vivian Siqueira Dutra, Graduate Program in Entomology, INPA; Beatriz Ronchi-Teles, INPA; MARCOS VINICIUS BASTOS GARCIA, CPAA; RICARDO ADAIME DA SILVA, CPAF-AP; Janisete Gomes Silva, Universidade Estadual de Santa Cruz. |
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Título: |
Native hosts and parasitoids associated with Anastrepha fractura and other Anastrepha Species (Diptera: Tephritidae) in the Brazilian Amazon. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
The Florida Entomologist, v. 96, n. 1, p. 270-273, 2013. |
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DOI: |
http://dx.doi.org/10.1653/024.096.0144 |
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Idioma: |
Inglês |
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Conteúdo: |
The Brazilian Amazon region comprises an exceptionally high diversity of angiosperms, among which approximately 180 described species, both native and exotic that can be potential fruit fly hosts (Silva & Ronchi-Teles 2000). Currently, 60 Anastrepha species have been recorded in the Brazilian Amazon region, of which about 30 species are endemic (Trindade & Uchôa 2011; Zucchi et al. 2011). Here we report on new host/fruit fly/parasitoid associations for some Anastrepha species for both Brazil and the Amazon region. A total of 4,137 fruit (73.9 kg) from 40 different native and introduced plant species in 19 families were collected from 2008 through 2011 in Manaus (S 03° 06' 07" W 60° 01' 30"), Maués (S 03° 23' 01" W 57° 43' 07"), Presidente Figueiredo (S 02° 02' 04" W 60° 01' 30"), and São Gabriel da Cachoeira (S 00° 07' 49" W 7° 05' 21") in the state of Amazonas; in Porto Velho (S 08° 45' 43" W 63° 54' 14") in the state of Rondônia, and in Boa Vista (S 02° 49' 11" W 60° 40' 24") in the state of Roraima. Fallen fruit, both ripe and ripening, were collected randomly from the ground under tree canopies within the forest. Cassava fruits were collected in an area adjacent to the forest in Manaus (Amazonas) and Porto Velho (Rondônia). Adult flies and parasitoids were reared from collected fruits following methods described in Ronchi-Teles et al. (2011). Voucher specimens were deposited at the Coleção de Invertebrados of the Instituto Nacional de Pesquisas da Amazônia. A total of 3,470 fruit (63.3 kg) were infested yielding 7,662 puparia from which 3,073 Anastrepha adults (1,469 males and 1,604 females), 669 braconid parasitoids, and 17 figitid parasitoids emerged. We report Anastrepha fractura Stone infesting fruit of Salacia sp. (Celastrales: Celastraceae) in association with Asobara anastrephae (Muesebeck), Doryctobracon brasiliensis (Szépligeti), and Opius bellus Gahan parasitoids for the first time (Table 1). Previously, A. fractura had been reported solely from Guyana (Stone 1942) and Amazonas, and the only known host and associated parasitoid were Maquira sclerophylla (Ducke) C.C. Berg (Rosales: Moraceae) and Doryctobracon areolatus (Szépligeti) (Costa et al. 2009), respectively. We also report Anastrepha distincta Greene infesting Inga cinnamomea Spruce ex Benth (Fabales: Fabaceae: Mimosoideae) in Brazil for the first time (Table 1), but which was not attacked by any of the parasitoid species herein reported. The remaining 13 Anastrepha species recovered during sampling (Table 1) had previously been reported infesting the hosts from which they were recovered here (Zucchi et al. 2011). MenosThe Brazilian Amazon region comprises an exceptionally high diversity of angiosperms, among which approximately 180 described species, both native and exotic that can be potential fruit fly hosts (Silva & Ronchi-Teles 2000). Currently, 60 Anastrepha species have been recorded in the Brazilian Amazon region, of which about 30 species are endemic (Trindade & Uchôa 2011; Zucchi et al. 2011). Here we report on new host/fruit fly/parasitoid associations for some Anastrepha species for both Brazil and the Amazon region. A total of 4,137 fruit (73.9 kg) from 40 different native and introduced plant species in 19 families were collected from 2008 through 2011 in Manaus (S 03° 06' 07" W 60° 01' 30"), Maués (S 03° 23' 01" W 57° 43' 07"), Presidente Figueiredo (S 02° 02' 04" W 60° 01' 30"), and São Gabriel da Cachoeira (S 00° 07' 49" W 7° 05' 21") in the state of Amazonas; in Porto Velho (S 08° 45' 43" W 63° 54' 14") in the state of Rondônia, and in Boa Vista (S 02° 49' 11" W 60° 40' 24") in the state of Roraima. Fallen fruit, both ripe and ripening, were collected randomly from the ground under tree canopies within the forest. Cassava fruits were collected in an area adjacent to the forest in Manaus (Amazonas) and Porto Velho (Rondônia). Adult flies and parasitoids were reared from collected fruits following methods described in Ronchi-Teles et al. (2011). Voucher specimens were deposited at the Coleção de Invertebrados of the Instituto Nacional de Pesquisas da Amazônia. A total of 3,... Mostrar Tudo |
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Palavras-Chave: |
Parasitoides. |
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Thesagro: |
Hospedeiro. |
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Categoria do assunto: |
--
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/82873/1/Dutra-et-al.-2013.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/82980/1/AP-2013-Native-hosts-and-parasitoids.pdf
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Marc: |
LEADER 03334naa a2200205 a 4500
001 1958061
005 2018-01-26
008 2013 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1653/024.096.0144$2DOI
100 1 $aDUTRA, V. S.
245 $aNative hosts and parasitoids associated with Anastrepha fractura and other Anastrepha Species (Diptera$bTephritidae) in the Brazilian Amazon.$h[electronic resource]
260 $c2013
520 $aThe Brazilian Amazon region comprises an exceptionally high diversity of angiosperms, among which approximately 180 described species, both native and exotic that can be potential fruit fly hosts (Silva & Ronchi-Teles 2000). Currently, 60 Anastrepha species have been recorded in the Brazilian Amazon region, of which about 30 species are endemic (Trindade & Uchôa 2011; Zucchi et al. 2011). Here we report on new host/fruit fly/parasitoid associations for some Anastrepha species for both Brazil and the Amazon region. A total of 4,137 fruit (73.9 kg) from 40 different native and introduced plant species in 19 families were collected from 2008 through 2011 in Manaus (S 03° 06' 07" W 60° 01' 30"), Maués (S 03° 23' 01" W 57° 43' 07"), Presidente Figueiredo (S 02° 02' 04" W 60° 01' 30"), and São Gabriel da Cachoeira (S 00° 07' 49" W 7° 05' 21") in the state of Amazonas; in Porto Velho (S 08° 45' 43" W 63° 54' 14") in the state of Rondônia, and in Boa Vista (S 02° 49' 11" W 60° 40' 24") in the state of Roraima. Fallen fruit, both ripe and ripening, were collected randomly from the ground under tree canopies within the forest. Cassava fruits were collected in an area adjacent to the forest in Manaus (Amazonas) and Porto Velho (Rondônia). Adult flies and parasitoids were reared from collected fruits following methods described in Ronchi-Teles et al. (2011). Voucher specimens were deposited at the Coleção de Invertebrados of the Instituto Nacional de Pesquisas da Amazônia. A total of 3,470 fruit (63.3 kg) were infested yielding 7,662 puparia from which 3,073 Anastrepha adults (1,469 males and 1,604 females), 669 braconid parasitoids, and 17 figitid parasitoids emerged. We report Anastrepha fractura Stone infesting fruit of Salacia sp. (Celastrales: Celastraceae) in association with Asobara anastrephae (Muesebeck), Doryctobracon brasiliensis (Szépligeti), and Opius bellus Gahan parasitoids for the first time (Table 1). Previously, A. fractura had been reported solely from Guyana (Stone 1942) and Amazonas, and the only known host and associated parasitoid were Maquira sclerophylla (Ducke) C.C. Berg (Rosales: Moraceae) and Doryctobracon areolatus (Szépligeti) (Costa et al. 2009), respectively. We also report Anastrepha distincta Greene infesting Inga cinnamomea Spruce ex Benth (Fabales: Fabaceae: Mimosoideae) in Brazil for the first time (Table 1), but which was not attacked by any of the parasitoid species herein reported. The remaining 13 Anastrepha species recovered during sampling (Table 1) had previously been reported infesting the hosts from which they were recovered here (Zucchi et al. 2011).
650 $aHospedeiro
653 $aParasitoides
700 1 $aRONCHI-TELES, B.
700 1 $aGARCIA, M. V. B.
700 1 $aADAIME, R.
700 1 $aSILVA, J. G.
773 $tThe Florida Entomologist$gv. 96, n. 1, p. 270-273, 2013.
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Registro original: |
Embrapa Amazônia Ocidental (CPAA) |
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