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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
22/03/2011 |
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Data da última atualização: |
05/06/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
COSTA, F. M.; BASTIANEL, M.; FREITAS-ASTUA, J.; KUBO, K. S.; MACHADO, M. A.; GONDIM JUNIOR, M. G. C. |
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Afiliação: |
F. M. Costa, UFRPE; Marinês Bastianel, APTA; JULIANA DE FREITAS ASTUA, CNPMF; Karen Sumire Kubo, IAC; Marcos Antonio Machado, APTA; Manoel Guedes Corrêa Gondim Júnior, APTA. |
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Título: |
Detection of coffee ringspot virus (CoRSV) in the mite vector by RT-qPCR. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In:INTERNATIONAL CONGRESS OF ACAROLOGY, 13., 2010, Recife. Abstracts book... Recife: CNPq: FACEPE: Banco do Nordeste: TWAS, 2010. p. 61-62. |
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Idioma: |
Inglês |
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Notas: |
Abstract 109 |
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Conteúdo: |
The mite Brevipalpus phoenicis (Geijskes, 1939) (Acari: Tenuipalpidae) is responsible for the transmission of coffee ringspot. There is a great interest in the study of Brevipalpus transmitted viruses (BrTVs), and the interaction between them, their vectors and hosts. Electron microscopy analyses suggest that CoRSV replicates in the mite tissue, what would characterize a persistent propagative virus-vector relationship. This study aimed to establish a transcriptase quantitative polymerase chain reaction (RT-qPCR) method to evaluate and compare the replication capacity of the virus in the vector. Total RNA was extracted from a sample of 100 mites reared in symptomatic plants for CoRSV using two different methods: CTAB and Nucleo Spin RNA XS Kit (Macherey-Nagel) and quantified in a NanoDrop 8000 (Thermo Scientific). |
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Thesagro: |
Brevipalpus Phoenicis; Vírus. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/31193/1/13CongASTUAID27574.pdf
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Marc: |
LEADER 01521nam a2200205 a 4500
001 1881980
005 2023-06-05
008 2010 bl uuuu u00u1 u #d
100 1 $aCOSTA, F. M.
245 $aDetection of coffee ringspot virus (CoRSV) in the mite vector by RT-qPCR.$h[electronic resource]
260 $aIn:INTERNATIONAL CONGRESS OF ACAROLOGY, 13., 2010, Recife. Abstracts book... Recife: CNPq: FACEPE: Banco do Nordeste: TWAS, 2010. p. 61-62.$c2010
500 $aAbstract 109
520 $aThe mite Brevipalpus phoenicis (Geijskes, 1939) (Acari: Tenuipalpidae) is responsible for the transmission of coffee ringspot. There is a great interest in the study of Brevipalpus transmitted viruses (BrTVs), and the interaction between them, their vectors and hosts. Electron microscopy analyses suggest that CoRSV replicates in the mite tissue, what would characterize a persistent propagative virus-vector relationship. This study aimed to establish a transcriptase quantitative polymerase chain reaction (RT-qPCR) method to evaluate and compare the replication capacity of the virus in the vector. Total RNA was extracted from a sample of 100 mites reared in symptomatic plants for CoRSV using two different methods: CTAB and Nucleo Spin RNA XS Kit (Macherey-Nagel) and quantified in a NanoDrop 8000 (Thermo Scientific).
650 $aBrevipalpus Phoenicis
650 $aVírus
700 1 $aBASTIANEL, M.
700 1 $aFREITAS-ASTUA, J.
700 1 $aKUBO, K. S.
700 1 $aMACHADO, M. A.
700 1 $aGONDIM JUNIOR, M. G. C.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| 1. |  | COSTA, F. M.; BASTIANEL, M.; FREITAS-ASTUA, J.; KUBO, K. S.; MACHADO, M. A.; GONDIM JUNIOR, M. G. C. Detection of coffee ringspot virus (CoRSV) in the mite vector by RT-qPCR. In:INTERNATIONAL CONGRESS OF ACAROLOGY, 13., 2010, Recife. Abstracts book... Recife: CNPq: FACEPE: Banco do Nordeste: TWAS, 2010. p. 61-62. Abstract 109| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 1 | |
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