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Registro Completo |
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Biblioteca(s): |
Embrapa Café; Embrapa Unidades Centrais. |
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Data corrente: |
22/02/2011 |
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Data da última atualização: |
09/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FRANCO, O. L.; PELEGRINI, P. B.; GOMES, C. P. C.; SOUZA, A.; COSTA, F. T.; DUMONT, G.; QUIRINO, B. F.; EIRA, M. T. S. da; MEHAT, A. |
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Afiliação: |
OCTAVIO L. FRANCO, UNIVERSIDADE CATÓLICA DE BRASÍLIA; PATRÍCIA B. PELEGRINI, UNIVERSIDADE CATÓLICA DE BRASÍLIA; CLARISSA, P. C. GOMES, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ANDRÉA SOUZA, UNIVERSIDADE CATÓLICA DE BRASÍLIA; FABIO T. COSTA, UNIVERSIDADE CATÓLICA DE BRASÍLIA; GILBERTO DUMONT, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; BETANIA FERRAZ QUIRINO, CNPAE; MIRIAN THEREZINHA SOUZA DA EIRA, SAPC. |
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Título: |
Proteomic evaluation of coffee zygotic embryos in two different stages of seed development. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Plant Physiology and Biochemistry, v. 47, p. 1046-1050, 2009. |
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Idioma: |
Inglês |
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Conteúdo: |
Coffee seed development is accompanied by severe modifications in water soluble proteins, several of these being associated to a specific developmental stage. For this reason, a proteomic approach has been used to describe spatial-temporal proteome modifications in zygotic embryos at different stages of seed development. Embryos from Coffea arabica seeds were harvested in two different developmental stages: stage 1 at 210 days after anthesis and stage 2 at 255 days. Total proteins were extracted and submitted to 2-DE. From these gels, several spots were identified by mass spectrometry including kinases, MYB transcription factor and enzymes involved in metabolic pathways. All proteins identified seem to affect coffee development in different ways, being directly involved in plant growth or used as an intermediate in some metabolic pathway that, indirectly, will influence coffee development. This is the first work using two-dimensional electrophoresis followed by mass spectrometry analyses that evaluates the expression of proteins during coffee zygotic embryos development. Data here reported supply some light over coffee development and could be used in a near future to improve coffee plants? growth and development by molecular strategies. |
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Palavras-Chave: |
Coffee. |
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Thesagro: |
Coffea Arábica; Coffea Canephora. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/29277/1/Proteomic-evaluation.pdf
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Marc: |
LEADER 01982naa a2200253 a 4500
001 1880392
005 2024-01-09
008 2009 bl uuuu u00u1 u #d
100 1 $aFRANCO, O. L.
245 $aProteomic evaluation of coffee zygotic embryos in two different stages of seed development.$h[electronic resource]
260 $c2009
520 $aCoffee seed development is accompanied by severe modifications in water soluble proteins, several of these being associated to a specific developmental stage. For this reason, a proteomic approach has been used to describe spatial-temporal proteome modifications in zygotic embryos at different stages of seed development. Embryos from Coffea arabica seeds were harvested in two different developmental stages: stage 1 at 210 days after anthesis and stage 2 at 255 days. Total proteins were extracted and submitted to 2-DE. From these gels, several spots were identified by mass spectrometry including kinases, MYB transcription factor and enzymes involved in metabolic pathways. All proteins identified seem to affect coffee development in different ways, being directly involved in plant growth or used as an intermediate in some metabolic pathway that, indirectly, will influence coffee development. This is the first work using two-dimensional electrophoresis followed by mass spectrometry analyses that evaluates the expression of proteins during coffee zygotic embryos development. Data here reported supply some light over coffee development and could be used in a near future to improve coffee plants? growth and development by molecular strategies.
650 $aCoffea Arábica
650 $aCoffea Canephora
653 $aCoffee
700 1 $aPELEGRINI, P. B.
700 1 $aGOMES, C. P. C.
700 1 $aSOUZA, A.
700 1 $aCOSTA, F. T.
700 1 $aDUMONT, G.
700 1 $aQUIRINO, B. F.
700 1 $aEIRA, M. T. S. da
700 1 $aMEHAT, A.
773 $tPlant Physiology and Biochemistry$gv. 47, p. 1046-1050, 2009.
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Registro original: |
Embrapa Café (CNPCa) |
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| 1. |  | FRANCO, O. L.; PELEGRINI, P. B.; GOMES, C. P. C.; SOUZA, A.; COSTA, F. T.; DUMONT, G.; QUIRINO, B. F.; EIRA, M. T. S. da; MEHAT, A. Proteomic evaluation of coffee zygotic embryos in two different stages of seed development. Plant Physiology and Biochemistry, v. 47, p. 1046-1050, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Café. |
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