| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
20/12/2010 |
|
Data da última atualização: |
20/01/2011 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
KUBO, K. S.; ARENA, G. D.; KITAJIMA, E. W; MACHADO, M. A.; FREITAS-ASTUA, J. de. |
|
Afiliação: |
Karen Sumire Kubo, APTA; APTA; Elliot Watanabe Kitajima, ESALQ; Marcos A. Machado, APTA; JULIANA DE FREITAS ASTUA, CNPMF. |
|
Título: |
Transmission of citrus leprosis virus c (CiLV-C) to Arabidopsis thaliana by Brevipalpus phoenicis mites. |
|
Ano de publicação: |
2010 |
|
Fonte/Imprenta: |
In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. |
|
Idioma: |
Inglês |
|
Notas: |
085 PS1
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010 |
|
Conteúdo: |
For many years, CiLV-C was classified as an unassigned rhabdovirus. After the complete sequencing of its genome, CiLV-C was proposed as the type member of a new genus of plant viruses, Cilevirus. This virus was believed to have a very narrow host range, but recent studies have shown that several plant species outside the genus Citrus are hosts of CiLV-C. Viral transmission to citrus plants is time consuming and the way plants defend themselves against CiLV-C is still largely unknown, despite recent efforts to evaluate the response of citrus to this pathogen. With the objective to use a model plant to evaluate the response to this disease, we tested whether or not Arabidopsis thaliana could be a host for the virus. We infested the Columbia ecotype with one, two, three, five or ten viruliferous mites for CiLV-C, with six biological repetitions of each treatment. This experiment was evaluated daily in order to verify the development of symptoms. Necrotic localized lesions started to appear in plants eight days after infestation with two mites. By the tenth day, 16% of the plants infested with ten mites, 32% of the plants with five mites and 50% of the plants with three, two and one mites developed necrotic lesions. Samples were processed for transmission electron microcopy (TEM) analyses and RT-PCR with primers designed to amplify a fragment within the movement protein gene of the virus was performed to assure that the lesions were caused by CiLV-C. It was possible to detect the virus in all symptomatic plants, both by RT-PCR and TEM. MenosFor many years, CiLV-C was classified as an unassigned rhabdovirus. After the complete sequencing of its genome, CiLV-C was proposed as the type member of a new genus of plant viruses, Cilevirus. This virus was believed to have a very narrow host range, but recent studies have shown that several plant species outside the genus Citrus are hosts of CiLV-C. Viral transmission to citrus plants is time consuming and the way plants defend themselves against CiLV-C is still largely unknown, despite recent efforts to evaluate the response of citrus to this pathogen. With the objective to use a model plant to evaluate the response to this disease, we tested whether or not Arabidopsis thaliana could be a host for the virus. We infested the Columbia ecotype with one, two, three, five or ten viruliferous mites for CiLV-C, with six biological repetitions of each treatment. This experiment was evaluated daily in order to verify the development of symptoms. Necrotic localized lesions started to appear in plants eight days after infestation with two mites. By the tenth day, 16% of the plants infested with ten mites, 32% of the plants with five mites and 50% of the plants with three, two and one mites developed necrotic lesions. Samples were processed for transmission electron microcopy (TEM) analyses and RT-PCR with primers designed to amplify a fragment within the movement protein gene of the virus was performed to assure that the lesions were caused by CiLV-C. It was possible to detect t... Mostrar Tudo |
|
Palavras-Chave: |
Plant disease. |
|
Thesagro: |
Doença de Planta; Fruta Cítrica; Vírus. |
|
Thesaurus Nal: |
Arabidopsis thaliana; Brevipalpus. |
|
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
|
Marc: |
LEADER 02444naa a2200253 a 4500
001 1870502
005 2011-01-20
008 2010 bl uuuu u00u1 u #d
100 1 $aKUBO, K. S.
245 $aTransmission of citrus leprosis virus c (CiLV-C) to Arabidopsis thaliana by Brevipalpus phoenicis mites.
260 $c2010
500 $a085 PS1 Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010
520 $aFor many years, CiLV-C was classified as an unassigned rhabdovirus. After the complete sequencing of its genome, CiLV-C was proposed as the type member of a new genus of plant viruses, Cilevirus. This virus was believed to have a very narrow host range, but recent studies have shown that several plant species outside the genus Citrus are hosts of CiLV-C. Viral transmission to citrus plants is time consuming and the way plants defend themselves against CiLV-C is still largely unknown, despite recent efforts to evaluate the response of citrus to this pathogen. With the objective to use a model plant to evaluate the response to this disease, we tested whether or not Arabidopsis thaliana could be a host for the virus. We infested the Columbia ecotype with one, two, three, five or ten viruliferous mites for CiLV-C, with six biological repetitions of each treatment. This experiment was evaluated daily in order to verify the development of symptoms. Necrotic localized lesions started to appear in plants eight days after infestation with two mites. By the tenth day, 16% of the plants infested with ten mites, 32% of the plants with five mites and 50% of the plants with three, two and one mites developed necrotic lesions. Samples were processed for transmission electron microcopy (TEM) analyses and RT-PCR with primers designed to amplify a fragment within the movement protein gene of the virus was performed to assure that the lesions were caused by CiLV-C. It was possible to detect the virus in all symptomatic plants, both by RT-PCR and TEM.
650 $aArabidopsis thaliana
650 $aBrevipalpus
650 $aDoença de Planta
650 $aFruta Cítrica
650 $aVírus
653 $aPlant disease
700 1 $aARENA, G. D.
700 1 $aKITAJIMA, E. W
700 1 $aMACHADO, M. A.
700 1 $aFREITAS-ASTUA, J. de
773 $tIn: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 108 | |
| 21. |  | LOCALI-FABRIS, E. C.; MAFRA, V. S.; MARTINS, P. K.; RIBEIRO- ALVES, M.; FRANCISCO, C. S.; FREITAS ASTUA, J. de; KISHI, L. T.; MACHADO, M. A. Gene set enrichment analysis in sweet orange plants infected by Candidatus Liberibacter americanus. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 0058 PSO
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
|    |
| 22. | | BASTIANEL, M.; NOVELLI, V. M.; FREITAS-ASTUA, J. de. Field assessments reveal different levels of susceptibility among mandarins and hybrids to Citrus Leprosis Virus c. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 129.PS2
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 23. | | FREITAS ASTUA, J. de; KUBO, K. S.; KITAJIMA, E. W.; BASTIANEL, M.; COSTA, F. M.; NOVELLI, V. M.; MACHADO, M. A. The leprosis pathosystem: interactions between host, virus and vector. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS,18., CAMPINAS, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 009 PSO
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 24. | | PEREIRA, F. M. V.; MILORI, D. M. B. P.; PEREIRA-FILHO, E. R.; VENÂNCIO, A. L.; RUSSO, M. de S. T.; MARTINS, P. K.; FREITAS-ASTÚA, J. Fluorescence images combined to chemometrics for the investigation of plants inoculated with candidatus liberibacter asiaticus In: CHEMOMETRICS IN ANALYTICAL CHEMISTRY - CAC, 12., 2010, Antwerp, Belgium. Book of abstracts... Antwerp: Universiteit Antwerpen, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
|  |
| 25. | | CÁCERES, S.; AGUIRRE, A.; COSTA, N.; SEGAÑA, L. G.; FARIÑA, N.; FREITAS ASTUA, J. de; MORAES, G. J.; TASSI, A. D.; KITAJIMA, E. W. Present situation of the citrus leprosis in Argentina and Paraguay. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 054 PSO.
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 26. | | NOVELLI, V. M.; FREITAS-ASTUA, J.; RIBEIRO, B. M.; LOCALI-FABRIS, E. C.; NAVIA, D.; KITAJIMA, E. W.; MACHADO, M. A. Prevalence of the Cardinium symbiont and genetic variability of Brevipalpus phoenicis (Acari: Tenuipalpidae) populations from Brazil. In:INTERNATIONAL CONGRESS OF ACAROLOGY, 13., 2010, Recife. Abstracts book... Recife: CNPq: FACEPE: Banco do Nordeste: TWAS, 2010. p. 181-182. Abstract 335| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 28. | | CORREA, P. H.; MARTINS, P. K.; ARENA, G. D.; MIGUEL, M. C.; MUTTI, H. S.; MACHADO, M. A.; FREITAS-ASTUA, J. Diagnóstico molecular de huanglongbing dos citros, via qPCR, em mudas de valência sobre Swingle. In:CONGRESSO BRASILEIRO DE FRUTICULTURA, 21., 2010, Natal. Frutas: saúde, inovação e responsabilidade: anais. Natal: Sociedade Brasileira de Fruticultura, 2010. pdf 1867| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 29. | | CUNHA, B. A.; NUNES, M. A.; FREITAS-ASTUA, J. de; BERGAMINI, M. P.; BASTIANEL, M.; NOVELLI, V. M. Different symptomatic tissues as sources of inoculum to citrus leprosis virus c (CILV-C). In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 128-PS2
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 30. | | RODRIGUES, A. S.; BARBOSA, C. de J.; SOARES FILHO, W. dos S.; FREITAS-ASTUA, J. de. Citrus rootstock hybrids tolerants to citrus tristeza virus and Bahia bark scaling disease. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 106_PS2
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 32. | | KUBO, K. S.; ARENA, G. D.; KITAJIMA, E. W; MACHADO, M. A.; FREITAS-ASTUA, J. de. Transmission of citrus leprosis virus c (CiLV-C) to Arabidopsis thaliana by Brevipalpus phoenicis mites. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 085 PS1
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 33. | | BASTIANEL, M.; PEREIRA, J. A.; NOVELLI, V. M.; ANTONIOLI-LUIZON, R.; AZEVEDO, F. A.; FREITAS-ASTUA, J. Resposta de tangerinas e híbridos ao virus da leprose dos citros. In:CONGRESSO BRASILEIRO DE FRUTICULTURA, 21., 2010, Natal. Frutas: saúde, inovação e responsabilidade: anais. Natal: Sociedade Brasileira de Fruticultura, 2010. pdf 1458| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 34. | | RUSSO, M. S. T.; CARDINALI, M.; MILORI, D. M. B. P.; PEREIRA, F. M. V.; MARTINS, P. K.; FREITAS-ASTÚA, J. Study of laser-induced fluorescence imaging in asymptomatic leaves from citrus plants inoculated with candidatus liberibacter. Citrus research & technology, Cordeirópolis, v. 31, suplemento, p. 122, 2010. Proceedings of the 18 conference international organization citrus virologists, 2010, Campinas.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação; Embrapa Mandioca e Fruticultura. |
| |
| 37. | | MASCARENHAS, G.; BARBOSA, C. de J.; KITAJIMA, E. W.; FREITAS-ÁSTUA, J. Alta eficiência de transmissão mecânica e por vetor do vírus da pinta verde (PFGSV) para maracujazeiro. Tropical Plant Pathology, Brasília, DF, v. 34, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009. Suplemento. Resumo 911.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 39. | | KUBO, K. S.; STUART, R. M.; FREITAS-ASTÚA, J.; ANTONIOLI-LUIZON, R.; LOCALI-FABRIS, E. C.; COLETTA-FILHO, H. D.; MACHADO, M. A.; KITAJIMA, E. W. Evaluation of the genetic variability of orchid fleck virus by single-strand conformational polymorphism analysis and nucleotide sequencing of a fragment from the nucleocapsid. Archives of Virology, v. 154, n. 6, p. 1009-1014, mai. 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 40. | | PEREIRA, J. A.; ANTONIOLO-LUIZON, R.; LOCALI-FABRIS, E. C.; KUBO, K. S.; MACHADO, M. A.; FREITAS-ÁSTUA, J. Estudo da variabilidade genética da possível capa protéica do Citrus leprosis virus virus C (CiLV-C) em espécies de citros de 14 estados brasileiros. Tropical Plant Pathology, Brasília, DF, v. 34, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009. Suplemento. Resumo 928.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| Registros recuperados : 108 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
