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 | Acesso ao texto completo restrito à biblioteca da Embrapa Trigo. Para informações adicionais entre em contato com cnpt.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
13/12/2010 |
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Data da última atualização: |
13/11/2014 |
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Autoria: |
ENDER, A.; SCHWENK, K.; STADLER, T.; STREIT, B.; SCHIERWATER, B. |
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Afiliação: |
ANDREA ENDER, J. W. Goethe Universität; KLAUS SCHWENK, Netherlands Institute of Ecology; THOMAS STÄDLER, J. W. Goethe Universität; BRUNO STREIT, J. W. Goethe Universität; BERND SCHIERWATER, Yale University. |
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Título: |
RAPD identification of microsatellites in Daphnia. |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Molecular Ecology, v. 5, n. 3, p. 437-441, 1996. |
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Idioma: |
Inglês |
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Conteúdo: |
Simple sequence repeats (SSRs, or microsatellites) have been constantly gaining importance as single-locus DNA markers in population genetics and behavioural ecology. We tested a PCR-based strategy for finding microsatellite loci in anonymous genomes, which avoids genomic library construction and screening, and the need for larger amounts of DNA. In the first step, parts of a genome are randomly amplified with arbitrary 10mer primers using RAPD fingerprinting. Labelled SSR-oligonucleotides serve as probes to detect complementary sequences in RAPD products by means of Southern analyses. Subsequently, positive RAPD fragments of suitable size are cloned and sequenced. Using GA and GT probes, we applied this approach to waterfleas (Daphnia) and revealed 37 hybridization signals in 20 RAPD profiles. Thirteen positive RAPD fragments from three Daphnia species and two hybrid 'species' were cloned and sequenced. In all cases simple sequence repeats were detected. We characterized seven perfect repeat loci, which were found to be polymorphic within and between species. |
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Palavras-Chave: |
Microsatellites; PCR; Simple sequence repeats. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01624naa a2200205 a 4500
001 1869478
005 2014-11-13
008 1996 bl --- 0-- u #d
100 1 $aENDER, A.
245 $aRAPD identification of microsatellites in Daphnia.$h[electronic resource]
260 $c1996
520 $aSimple sequence repeats (SSRs, or microsatellites) have been constantly gaining importance as single-locus DNA markers in population genetics and behavioural ecology. We tested a PCR-based strategy for finding microsatellite loci in anonymous genomes, which avoids genomic library construction and screening, and the need for larger amounts of DNA. In the first step, parts of a genome are randomly amplified with arbitrary 10mer primers using RAPD fingerprinting. Labelled SSR-oligonucleotides serve as probes to detect complementary sequences in RAPD products by means of Southern analyses. Subsequently, positive RAPD fragments of suitable size are cloned and sequenced. Using GA and GT probes, we applied this approach to waterfleas (Daphnia) and revealed 37 hybridization signals in 20 RAPD profiles. Thirteen positive RAPD fragments from three Daphnia species and two hybrid 'species' were cloned and sequenced. In all cases simple sequence repeats were detected. We characterized seven perfect repeat loci, which were found to be polymorphic within and between species.
653 $aMicrosatellites
653 $aPCR
653 $aSimple sequence repeats
700 1 $aSCHWENK, K.
700 1 $aSTADLER, T.
700 1 $aSTREIT, B.
700 1 $aSCHIERWATER, B.
773 $tMolecular Ecology$gv. 5, n. 3, p. 437-441, 1996.
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Registro original: |
Embrapa Trigo (CNPT) |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
26/08/2011 |
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Data da última atualização: |
11/11/2022 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
VALENCIA, L. I. O.; MEIRELLES, M. S. P.; MARTORANO, L. G. |
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Afiliação: |
LUIS IVÁN ORTIZ VALENCIA, UFRJ/IESC; MARGARETH SIMOES PENELLO MEIRELLES, EMBRAPA LABEX EUROPA / CIRAD / UERJ; LUCIETA GUERREIRO MARTORANO, CPATU. |
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Título: |
Avaliação do modelo de mudança do uso do solo CLUE-S na presença de dependência espacial. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE SENSORIAMENTO REMOTO, 15., 2011, Curitiba. Anais... São José dos Campos: INPE, 2011. |
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Idioma: |
Português |
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Conteúdo: |
Land use changes impacts global environmental changes worldwide. Land use and cover changes (LUCC) are complex dynamical processes. In general, LUCC depends on geographical, environmental, social, economical and political factors with effects in diverse temporal and spatial scales. Great efforts are being made to create LUCC models, both theoretically and computationality. An important step in LUCC models is to quantify local influence of driving forces over the probability of occurrence of a specific land use type. This probability is usually quantified by a Logistic Regression Model. This paper compares a Logistic approach with a Spatial Lag Regression Model (SLRM). Generally, both approaches select different driving forces but SLRM incorporates spatial neighborhood information which is ignored by a logistic regression. Based on a lineal trend scenario for land use demand, simulations for land use changes for Coxim micro basin, Mato Grosso do Sul, were generated using CLUE-S program under a logistic and spatial regression models for the period 2001-2011. Simulation results for both approaches revealed elevated concordance indexes, high global accuracy and Kappa index, with respect to land use for the reference year of 2004. Differences were observed for spatial distribution for land use simulations for year 2011. The spatial regression approach simulation for 2011 reached less discrepancy to the land use demand for that year. |
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Palavras-Chave: |
Modelo de regressão; Uso do solo. |
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Thesagro: |
Simulação. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/40610/1/AVALIACAO-DO-MODELO-MUDANCA-USO-SOLO-CLUE-S.pdf
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Marc: |
LEADER 02051nam a2200169 a 4500
001 1898949
005 2022-11-11
008 2011 bl uuuu u00u1 u #d
100 1 $aVALENCIA, L. I. O.
245 $aAvaliação do modelo de mudança do uso do solo CLUE-S na presença de dependência espacial.$h[electronic resource]
260 $aIn: SIMPÓSIO BRASILEIRO DE SENSORIAMENTO REMOTO, 15., 2011, Curitiba. Anais... São José dos Campos: INPE$c2011
520 $aLand use changes impacts global environmental changes worldwide. Land use and cover changes (LUCC) are complex dynamical processes. In general, LUCC depends on geographical, environmental, social, economical and political factors with effects in diverse temporal and spatial scales. Great efforts are being made to create LUCC models, both theoretically and computationality. An important step in LUCC models is to quantify local influence of driving forces over the probability of occurrence of a specific land use type. This probability is usually quantified by a Logistic Regression Model. This paper compares a Logistic approach with a Spatial Lag Regression Model (SLRM). Generally, both approaches select different driving forces but SLRM incorporates spatial neighborhood information which is ignored by a logistic regression. Based on a lineal trend scenario for land use demand, simulations for land use changes for Coxim micro basin, Mato Grosso do Sul, were generated using CLUE-S program under a logistic and spatial regression models for the period 2001-2011. Simulation results for both approaches revealed elevated concordance indexes, high global accuracy and Kappa index, with respect to land use for the reference year of 2004. Differences were observed for spatial distribution for land use simulations for year 2011. The spatial regression approach simulation for 2011 reached less discrepancy to the land use demand for that year.
650 $aSimulação
653 $aModelo de regressão
653 $aUso do solo
700 1 $aMEIRELLES, M. S. P.
700 1 $aMARTORANO, L. G.
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Embrapa Amazônia Oriental (CPATU) |
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