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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
07/12/2010 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TIMBÓ, A. L. de O.; DAVIDE, L. C.; PINTO, J. E. B. P.; PEREIRA, A. V. |
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Afiliação: |
ANA LUIZA DE OLIVEIRA TIMBÓ, UFLA; LISETE CHAMA DAVIDE, UFLA; JOSÉ EDUARDO BRASIL PEREIRA PINTO, UFLA; ANTONIO VANDER PEREIRA, CNPGL. |
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Título: |
Protoplast production from napier grass and pearl millet triploid hybrids. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Ciência e Agrotecnologia, v. 34, n. 5, p. 1219-1223, set./out. 2010. |
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DOI: |
https://doi.org/10.1590/S1413-70542010000500020 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this work was to obtain protoplasts from napier grass and pearl millet triploid hybrids as a basis for future studies on chromosomal duplication. Explants were taken from mesophyll of in vitro- and in vivo-cultured plants or from calli of two triploid hybrids (H1 and H2), which were treated with enzymatic solutions containing different concentrations of cellulase R-10 (0.5, 1.0, 1.5 and 2.0%) with an additional 0.2% macerozyme and 0.1% driselase or 1.0% pectolyase Y-23 and 0.5% hemicellulase. Enzymatic digestion was monitored once every hour for five hours. Protoplasts were obtained from in vitro and in vivo leaflets of both triploid hybrids, and in vitro leaflets were the best explant sources. The quantity of produced protoplasts varied according to the hybrid, the enzymatic solution and the treatment time. |
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Palavras-Chave: |
In vitro cultivation; Interspecific hybrids. |
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Thesagro: |
Pennisetum Glaucum; Pennisetum Purpureum. |
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Thesaurus Nal: |
forage. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/23923/1/Vander-2010-Cienc-Agrotec.pdf
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Marc: |
LEADER 01567naa a2200229 a 4500
001 1868965
005 2024-02-06
008 2010 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/S1413-70542010000500020$2DOI
100 1 $aTIMBÓ, A. L. de O.
245 $aProtoplast production from napier grass and pearl millet triploid hybrids.$h[electronic resource]
260 $c2010
520 $aThe objective of this work was to obtain protoplasts from napier grass and pearl millet triploid hybrids as a basis for future studies on chromosomal duplication. Explants were taken from mesophyll of in vitro- and in vivo-cultured plants or from calli of two triploid hybrids (H1 and H2), which were treated with enzymatic solutions containing different concentrations of cellulase R-10 (0.5, 1.0, 1.5 and 2.0%) with an additional 0.2% macerozyme and 0.1% driselase or 1.0% pectolyase Y-23 and 0.5% hemicellulase. Enzymatic digestion was monitored once every hour for five hours. Protoplasts were obtained from in vitro and in vivo leaflets of both triploid hybrids, and in vitro leaflets were the best explant sources. The quantity of produced protoplasts varied according to the hybrid, the enzymatic solution and the treatment time.
650 $aforage
650 $aPennisetum Glaucum
650 $aPennisetum Purpureum
653 $aIn vitro cultivation
653 $aInterspecific hybrids
700 1 $aDAVIDE, L. C.
700 1 $aPINTO, J. E. B. P.
700 1 $aPEREIRA, A. V.
773 $tCiência e Agrotecnologia$gv. 34, n. 5, p. 1219-1223, set./out. 2010.
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Embrapa Gado de Leite (CNPGL) |
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