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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
01/11/2010 |
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Data da última atualização: |
29/06/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
BRITO, L. G.; BARBIERI, F. da S.; OLIVEIRA, M. C. de S.; GUERRERO, F. D. |
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Afiliação: |
LUCIANA GATTO BRITO, CPAF-RO; FABIO DA SILVA BARBIERI, CPAF-RO; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; F. D. GUERRERO, LIVESTOCK INSECTS RESEARCH LABORATORY/KERRVILLE - TX. |
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Título: |
Pasa assay for diagnosing insecticide resistance in the horn fly population in Rondonia. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., Campo Grande. Anais... Campo Grqande: CBPV, 2010. |
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Páginas: |
p. 127 - 128. |
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Idioma: |
Português |
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Conteúdo: |
Knockdown (kdr) resistance is caused by a reduction in the sensitivity of the insectnnervous system to pyrethroids. Kdr resistance in field populations of horn flies can severely limit pyrethroid usefulness in fly control programs. Early detection and characterization of kdr resistance are critical to the development of resistance management strategies. Studies at the Embrapa Rondonia experimental farm, Porto Velho, RO and at Knipling-Bushland U.S. Livestock Insects Research Laboratory, USDA/ARS, Kerrville, TX were conducted to verify the genotypes of the Embrapa Rondonia horn fly population. First, the population was assessed using cypermethrin-impregnated filter papers produced at the Embrapa Rondonia Animal Health Laboratory. Horn flies from an untreated cattle herd were caught with a sweep net and used for bioassays. Flies were exposed for two hours to filter papers treated with technical grade cypermethrin in acetone, using cypermethrin concentrations from 0.01 ?g to 3,200 ?g/cm2. Control flies were exposed to filter papers treated only with acetone. Three groups of ten flies were exposed at each concentration. All flies exposed to cypermethrin concentrations between 800-3,200 ?g/cm2 died. Genomic DNA was isolated from individual adult flies that survived bioassay concentrations of 200-400 ?g/cm2 and 30 flies tested by PASA (PCR amplification of specific alleles) assay for the presence of a specific nucleotide substitution in the sodium channel gene sequence that has been associated with kdr resistance in horn flies. PASA was performed using two parallel PCRs, with each PCR containing three sets of primers, and genomic DNA to detect pyrethroid resistance-associated nucleotide differences in individual flies. Two primers, FG 234 and FG 243, provided a positive control PCR product while the products of primer FG 138 with primer FG 130 (reaction 1) or with primer FG 134 (reaction 2) produced diagnostic products for genotyping the kdr allele. Reaction products were visualized after 4% agarose gel electrophoresis followed by UV illumination after staining with Syber Green. The kdr allele was not detected in flies from the Embrapa Rondonia population, which was considered a pyrethroid susceptible homozygous (SS) population. This result was expected for this horn fly population as there is no report of treatment with pyrethroids in the last six years. MenosKnockdown (kdr) resistance is caused by a reduction in the sensitivity of the insectnnervous system to pyrethroids. Kdr resistance in field populations of horn flies can severely limit pyrethroid usefulness in fly control programs. Early detection and characterization of kdr resistance are critical to the development of resistance management strategies. Studies at the Embrapa Rondonia experimental farm, Porto Velho, RO and at Knipling-Bushland U.S. Livestock Insects Research Laboratory, USDA/ARS, Kerrville, TX were conducted to verify the genotypes of the Embrapa Rondonia horn fly population. First, the population was assessed using cypermethrin-impregnated filter papers produced at the Embrapa Rondonia Animal Health Laboratory. Horn flies from an untreated cattle herd were caught with a sweep net and used for bioassays. Flies were exposed for two hours to filter papers treated with technical grade cypermethrin in acetone, using cypermethrin concentrations from 0.01 ?g to 3,200 ?g/cm2. Control flies were exposed to filter papers treated only with acetone. Three groups of ten flies were exposed at each concentration. All flies exposed to cypermethrin concentrations between 800-3,200 ?g/cm2 died. Genomic DNA was isolated from individual adult flies that survived bioassay concentrations of 200-400 ?g/cm2 and 30 flies tested by PASA (PCR amplification of specific alleles) assay for the presence of a specific nucleotide substitution in the sodium channel gene sequence that has be... Mostrar Tudo |
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Palavras-Chave: |
Allele characterization; Kdr; Pyrethroid resistance; Rondonia. |
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Thesagro: |
Haematobia Irritans. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/865775/1/OK-PasaAssayDiagnosing.pdf
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Marc: |
LEADER 03107nam a2200217 a 4500
001 1865775
005 2023-06-29
008 2010 bl uuuu u00u1 u #d
100 1 $aBRITO, L. G.
245 $aPasa assay for diagnosing insecticide resistance in the horn fly population in Rondonia.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., Campo Grande. Anais... Campo Grqande: CBPV$c2010
300 $ap. 127 - 128.
520 $aKnockdown (kdr) resistance is caused by a reduction in the sensitivity of the insectnnervous system to pyrethroids. Kdr resistance in field populations of horn flies can severely limit pyrethroid usefulness in fly control programs. Early detection and characterization of kdr resistance are critical to the development of resistance management strategies. Studies at the Embrapa Rondonia experimental farm, Porto Velho, RO and at Knipling-Bushland U.S. Livestock Insects Research Laboratory, USDA/ARS, Kerrville, TX were conducted to verify the genotypes of the Embrapa Rondonia horn fly population. First, the population was assessed using cypermethrin-impregnated filter papers produced at the Embrapa Rondonia Animal Health Laboratory. Horn flies from an untreated cattle herd were caught with a sweep net and used for bioassays. Flies were exposed for two hours to filter papers treated with technical grade cypermethrin in acetone, using cypermethrin concentrations from 0.01 ?g to 3,200 ?g/cm2. Control flies were exposed to filter papers treated only with acetone. Three groups of ten flies were exposed at each concentration. All flies exposed to cypermethrin concentrations between 800-3,200 ?g/cm2 died. Genomic DNA was isolated from individual adult flies that survived bioassay concentrations of 200-400 ?g/cm2 and 30 flies tested by PASA (PCR amplification of specific alleles) assay for the presence of a specific nucleotide substitution in the sodium channel gene sequence that has been associated with kdr resistance in horn flies. PASA was performed using two parallel PCRs, with each PCR containing three sets of primers, and genomic DNA to detect pyrethroid resistance-associated nucleotide differences in individual flies. Two primers, FG 234 and FG 243, provided a positive control PCR product while the products of primer FG 138 with primer FG 130 (reaction 1) or with primer FG 134 (reaction 2) produced diagnostic products for genotyping the kdr allele. Reaction products were visualized after 4% agarose gel electrophoresis followed by UV illumination after staining with Syber Green. The kdr allele was not detected in flies from the Embrapa Rondonia population, which was considered a pyrethroid susceptible homozygous (SS) population. This result was expected for this horn fly population as there is no report of treatment with pyrethroids in the last six years.
650 $aHaematobia Irritans
653 $aAllele characterization
653 $aKdr
653 $aPyrethroid resistance
653 $aRondonia
700 1 $aBARBIERI, F. da S.
700 1 $aOLIVEIRA, M. C. de S.
700 1 $aGUERRERO, F. D.
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