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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
28/10/2010 |
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Data da última atualização: |
28/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ABREU, J. T.; MOURÃO, M. M.; SANTOS, C. E.; VELOSO, C. J. M.; RESENDE, J. S.; FLATSCHART, R. B.; FOLGUERAS-FLATSCHAR, A. V.; JUNIOR, S. N.; SANTORO, M. M.; MENDES, A. C. R.; FRANCO, G. R.; SILVA, A.; CAMPOS, A. B.; FERNANDEZ, S. |
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Afiliação: |
AUREA VALADARES FOLGUERAS FLATSCHAR, CNPMS. |
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Título: |
Molecular studies of the brazilian infectious bronchitis virus isolates. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Revista Brasileira de Ciência Avícola, Campinas, v. 12, n. 2, p. 107-110, 2010. |
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DOI: |
10.1590/S1516-635X2010000200005 |
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Idioma: |
Inglês |
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Conteúdo: |
Avian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading to the conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais before the official approval of vaccination (1980). MenosAvian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading to the conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais bef... Mostrar Tudo |
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Thesagro: |
Genética; Virus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32305/1/Molecular-studies.pdf
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Marc: |
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005 2018-05-28
008 2010 bl uuuu u00u1 u #d
024 7 $a10.1590/S1516-635X2010000200005$2DOI
100 1 $aABREU, J. T.
245 $aMolecular studies of the brazilian infectious bronchitis virus isolates.$h[electronic resource]
260 $c2010
520 $aAvian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading to the conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais before the official approval of vaccination (1980).
650 $aGenética
650 $aVirus
700 1 $aMOURÃO, M. M.
700 1 $aSANTOS, C. E.
700 1 $aVELOSO, C. J. M.
700 1 $aRESENDE, J. S.
700 1 $aFLATSCHART, R. B.
700 1 $aFOLGUERAS-FLATSCHAR, A. V.
700 1 $aJUNIOR, S. N.
700 1 $aSANTORO, M. M.
700 1 $aMENDES, A. C. R.
700 1 $aFRANCO, G. R.
700 1 $aSILVA, A.
700 1 $aCAMPOS, A. B.
700 1 $aFERNANDEZ, S.
773 $tRevista Brasileira de Ciência Avícola, Campinas$gv. 12, n. 2, p. 107-110, 2010.
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Embrapa Milho e Sorgo (CNPMS) |
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| 1. |  | ABREU, J. T.; MOURÃO, M. M.; SANTOS, C. E.; VELOSO, C. J. M.; RESENDE, J. S.; FLATSCHART, R. B.; FOLGUERAS-FLATSCHAR, A. V.; JUNIOR, S. N.; SANTORO, M. M.; MENDES, A. C. R.; FRANCO, G. R.; SILVA, A.; CAMPOS, A. B.; FERNANDEZ, S. Molecular studies of the brazilian infectious bronchitis virus isolates. Revista Brasileira de Ciência Avícola, Campinas, v. 12, n. 2, p. 107-110, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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