|
|
Registro Completo |
Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
26/07/2000 |
Data da última atualização: |
26/07/2000 |
Autoria: |
SILVA FILHO, D. F. da; MACHADO, F. M. |
Título: |
Cubiu (Solanum sessiliflorum Dun.). |
Ano de publicação: |
1997 |
Fonte/Imprenta: |
In: CARDOSO, M.O., coord. Hortalicas nao-convencionais da Amazonia. Brasilia: EMBRAPA-SPI / Manaus: EMBRAPA-CPAA, 1997. |
Páginas: |
p.97-104. |
Idioma: |
Português |
Conteúdo: |
Aspectos gerais; Caracteristicas botanicas e variedades; Exigencias de clima e solos; Propagacao e cultivo; Pragas e doencas; Colheita e comercializacao. |
Palavras-Chave: |
Amazonas; Brasil; Chemcial control; Cultivar; Cultivation; Cultivo; Diseases; Fungus; Harvest; Hortalica alternativa; Pest insects; Postharvest; Propagacao; Propagation; Rhyzoctonia solani; Sclerotium solfsii. |
Thesagro: |
Ácaro; Botânica; Clima; Colheita; Comercialização; Controle Químico; Cubiú; Doença; Fungicida; Fungo; Inseticida; Inseto; Pós-Colheita; Praga; Pulgão; Solanum Sessiliflorum; Solo; Vaquinha; Variedade. |
Thesaurus Nal: |
botany; Brazil; climate; fungicides; insecticides; marketing; Pythium; soil; varieties. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01764naa a2200673 a 4500 001 1768731 005 2000-07-26 008 1997 bl uuuu u00u1 u #d 100 1 $aSILVA FILHO, D. F. da 245 $aCubiu (Solanum sessiliflorum Dun.). 260 $c1997 300 $ap.97-104. 520 $aAspectos gerais; Caracteristicas botanicas e variedades; Exigencias de clima e solos; Propagacao e cultivo; Pragas e doencas; Colheita e comercializacao. 650 $abotany 650 $aBrazil 650 $aclimate 650 $afungicides 650 $ainsecticides 650 $amarketing 650 $aPythium 650 $asoil 650 $avarieties 650 $aÁcaro 650 $aBotânica 650 $aClima 650 $aColheita 650 $aComercialização 650 $aControle Químico 650 $aCubiú 650 $aDoença 650 $aFungicida 650 $aFungo 650 $aInseticida 650 $aInseto 650 $aPós-Colheita 650 $aPraga 650 $aPulgão 650 $aSolanum Sessiliflorum 650 $aSolo 650 $aVaquinha 650 $aVariedade 653 $aAmazonas 653 $aBrasil 653 $aChemcial control 653 $aCultivar 653 $aCultivation 653 $aCultivo 653 $aDiseases 653 $aFungus 653 $aHarvest 653 $aHortalica alternativa 653 $aPest insects 653 $aPostharvest 653 $aPropagacao 653 $aPropagation 653 $aRhyzoctonia solani 653 $aSclerotium solfsii 700 1 $aMACHADO, F. M. 773 $tIn: CARDOSO, M.O., coord. Hortalicas nao-convencionais da Amazonia. Brasilia: EMBRAPA-SPI / Manaus: EMBRAPA-CPAA, 1997.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Hortaliças (CNPH) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
28/09/2010 |
Data da última atualização: |
30/06/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; SOUZA, M. M.; LIMA, M. R. de; NICIURA, S. C. M.; GARCIA, J. M. |
Afiliação: |
N. Z. SARAIVA, UNESP/JABOTICABAL; C. S. OLIVEIRA, UNESP/JABOTICABAL; T. A. D. TETZNER, UNESP/JABOTICABAL; M. M. SOUZA, UNESP/JABOTICABAL; M. R. DE LIMA, UNESP/JABOTICABAL; SIMONE CRISTINA MEO NICIURA, CPPSE; J. M. GARCIA, UNESP/JABOTICABAL. |
Título: |
Bovine cytoplasts prepared by demecolcine-induced enucleation of activated oocytes. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
In: ANNUAL CONFERENCE OT THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 36., 2010, Cordoba. Proceedings... Córdoba: ITES, 2010; Reproduction, Fertility and development, v. 22, n. 1, 2010. |
Páginas: |
p. 197 |
Idioma: |
Português |
Conteúdo: |
One of the most critical steps of the standard NT procedure is the removal of oocyte chromosomes for the production of enucleated cytoplasts. This process involves ultraviolet (UV) irradiation, which causes damage to the membrane and intracellular components of bovine oocytes. The objective of the present study was to evaluate the use of demecolcine, a microtubule depolymerizing agent, for chemical-induced enucleation of activated bovine oocytes. In the first experiment, oocytes obtained from cow ovaries collected in a slaughterhouse were IVM in TCM-199 medium supplemented with 10% FCS, FSH, hCG, estradiol, pyruvate, and amikacin for 26 h; then, they were artificially activated with 5 ?M ionomycin for 5 min and 10 ?g mL-1 cycloheximide for 4 h. The following groups were established: control, C (no activation and no demecolcine); activated, A (exposure only to activating agents); and demecolcine-exposed oocytes, DEME (activation for 4 h and 0.05 ?g mL-1 demecolcine for 2 to 4 h after 0, 0.5, 1.0, 1.5, and 2.0 h of activation). The treatment DEME comprised the groups G1 (demecolcine from 0 to 2 h of activation; DEME 0-2 h); G2 (DEME 0-4 h); G3 (DEME 0.5-2.5 h); G4 (DEME 0.5-4 h); G5 (DEME 1-3 h); G6 (DEME 1-4 h); G7 (DEME 1.5-3.5 h); G8 (DEME 1.5-4 h); and G9 (DEME 2-4h). The oocytes were stained with 10 ?g mL-1 Hoechst 33342 for 15 min and enucleation rates (EN) were evaluated under epifluorescence microscope (330-385 nm).After EN evaluation, a second experiment was performed to verify the efficiency of demecolcine-induced enucleation in group G9, which showed the best result in the first experiment. After demecolcine treatment, the oocytes were incubated in HEPES-buffered SOF with 10% FCS and cytochalasin B for removal of the 2PB and minimal adjacent cytoplast under an inverted optical microscope. Traditional enucleation was performed on the control group without exposure of the oocytes to demecolcine. The same conditions were employed except that UV light was used to confirm enucleation. Samples of cytoplasts were stained with Hoechst for 15 min and analyzed for enucleation efficiency. Five and 3 replicates were evaluated, respectively, in experiments 1 and 2, and the results were analyzed by chi-square test in the statistical software Minitab®, release 14.1 (Minitab Inc., State College, PA, USA). A level of 5% significance was used. Regarding enucleation rates, all treated groups were significantly different compared with the C (0/114) and A (0/130) groups. Considering the DEME groups, G8 (46/109; 42.2%) and G9 (61/113; 54.0%) presented superior rates (P < 0.05) to all other groups (26.8 to 36.3%), but they were similar despite the great tendency (P = 0.07) to difference. We observed high efficiency (P < 0.05) of demecolcine-induced enucleation (90/110; 81.8%) compared with a traditional technique (61/95; 64.2%). In conclusion, the present study shows that demecolcine-induced enucleation of activated oocytes enables good rates of enucleation and has greater efficiency than the traditional technique as well as avoiding UV irradiation of the cytoplast. MenosOne of the most critical steps of the standard NT procedure is the removal of oocyte chromosomes for the production of enucleated cytoplasts. This process involves ultraviolet (UV) irradiation, which causes damage to the membrane and intracellular components of bovine oocytes. The objective of the present study was to evaluate the use of demecolcine, a microtubule depolymerizing agent, for chemical-induced enucleation of activated bovine oocytes. In the first experiment, oocytes obtained from cow ovaries collected in a slaughterhouse were IVM in TCM-199 medium supplemented with 10% FCS, FSH, hCG, estradiol, pyruvate, and amikacin for 26 h; then, they were artificially activated with 5 ?M ionomycin for 5 min and 10 ?g mL-1 cycloheximide for 4 h. The following groups were established: control, C (no activation and no demecolcine); activated, A (exposure only to activating agents); and demecolcine-exposed oocytes, DEME (activation for 4 h and 0.05 ?g mL-1 demecolcine for 2 to 4 h after 0, 0.5, 1.0, 1.5, and 2.0 h of activation). The treatment DEME comprised the groups G1 (demecolcine from 0 to 2 h of activation; DEME 0-2 h); G2 (DEME 0-4 h); G3 (DEME 0.5-2.5 h); G4 (DEME 0.5-4 h); G5 (DEME 1-3 h); G6 (DEME 1-4 h); G7 (DEME 1.5-3.5 h); G8 (DEME 1.5-4 h); and G9 (DEME 2-4h). The oocytes were stained with 10 ?g mL-1 Hoechst 33342 for 15 min and enucleation rates (EN) were evaluated under epifluorescence microscope (330-385 nm).After EN evaluation, a second experiment was performed... Mostrar Tudo |
Palavras-Chave: |
Bovine; Cytoplasts. |
Thesaurus NAL: |
oocytes. |
Categoria do assunto: |
W Química e Física |
Marc: |
LEADER 03900nam a2200229 a 4500 001 1863081 005 2023-06-30 008 2010 bl uuuu u00u1 u #d 100 1 $aSARAIVA, N. Z. 245 $aBovine cytoplasts prepared by demecolcine-induced enucleation of activated oocytes.$h[electronic resource] 260 $aIn: ANNUAL CONFERENCE OT THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 36., 2010, Cordoba. Proceedings... Córdoba: ITES, 2010; Reproduction, Fertility and development, v. 22, n. 1, 2010.$c2010 300 $ap. 197 520 $aOne of the most critical steps of the standard NT procedure is the removal of oocyte chromosomes for the production of enucleated cytoplasts. This process involves ultraviolet (UV) irradiation, which causes damage to the membrane and intracellular components of bovine oocytes. The objective of the present study was to evaluate the use of demecolcine, a microtubule depolymerizing agent, for chemical-induced enucleation of activated bovine oocytes. In the first experiment, oocytes obtained from cow ovaries collected in a slaughterhouse were IVM in TCM-199 medium supplemented with 10% FCS, FSH, hCG, estradiol, pyruvate, and amikacin for 26 h; then, they were artificially activated with 5 ?M ionomycin for 5 min and 10 ?g mL-1 cycloheximide for 4 h. The following groups were established: control, C (no activation and no demecolcine); activated, A (exposure only to activating agents); and demecolcine-exposed oocytes, DEME (activation for 4 h and 0.05 ?g mL-1 demecolcine for 2 to 4 h after 0, 0.5, 1.0, 1.5, and 2.0 h of activation). The treatment DEME comprised the groups G1 (demecolcine from 0 to 2 h of activation; DEME 0-2 h); G2 (DEME 0-4 h); G3 (DEME 0.5-2.5 h); G4 (DEME 0.5-4 h); G5 (DEME 1-3 h); G6 (DEME 1-4 h); G7 (DEME 1.5-3.5 h); G8 (DEME 1.5-4 h); and G9 (DEME 2-4h). The oocytes were stained with 10 ?g mL-1 Hoechst 33342 for 15 min and enucleation rates (EN) were evaluated under epifluorescence microscope (330-385 nm).After EN evaluation, a second experiment was performed to verify the efficiency of demecolcine-induced enucleation in group G9, which showed the best result in the first experiment. After demecolcine treatment, the oocytes were incubated in HEPES-buffered SOF with 10% FCS and cytochalasin B for removal of the 2PB and minimal adjacent cytoplast under an inverted optical microscope. Traditional enucleation was performed on the control group without exposure of the oocytes to demecolcine. The same conditions were employed except that UV light was used to confirm enucleation. Samples of cytoplasts were stained with Hoechst for 15 min and analyzed for enucleation efficiency. Five and 3 replicates were evaluated, respectively, in experiments 1 and 2, and the results were analyzed by chi-square test in the statistical software Minitab®, release 14.1 (Minitab Inc., State College, PA, USA). A level of 5% significance was used. Regarding enucleation rates, all treated groups were significantly different compared with the C (0/114) and A (0/130) groups. Considering the DEME groups, G8 (46/109; 42.2%) and G9 (61/113; 54.0%) presented superior rates (P < 0.05) to all other groups (26.8 to 36.3%), but they were similar despite the great tendency (P = 0.07) to difference. We observed high efficiency (P < 0.05) of demecolcine-induced enucleation (90/110; 81.8%) compared with a traditional technique (61/95; 64.2%). In conclusion, the present study shows that demecolcine-induced enucleation of activated oocytes enables good rates of enucleation and has greater efficiency than the traditional technique as well as avoiding UV irradiation of the cytoplast. 650 $aoocytes 653 $aBovine 653 $aCytoplasts 700 1 $aOLIVEIRA, C. S. 700 1 $aTETZNER, T. A. D. 700 1 $aSOUZA, M. M. 700 1 $aLIMA, M. R. de 700 1 $aNICIURA, S. C. M. 700 1 $aGARCIA, J. M.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|