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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
22/04/2010 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FONSECA, I.; SILVA, P. V.; LANGE, C. C.; GUIMARAES, M. F. M.; WELLER, M. M. D. C. A.; SOUSA, K. R. S.; LOPES, P. S.; GUIMARÃES, J. D.; GUIMARÃES, S. E. F. |
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Afiliação: |
ISABELA FONSECA, UFV; PRISCILA VENDRAMINI SILVA, UFV; CARLA CHRISTINE LANGE, CNPGL; MARTA FONSECA MARTINS GUIMARAES, CNPGL; MAYARA MORENA DEL CAMBRE AMARAL WELLER, UFV; KATIENE RÉGIA SILVA SOUSA, UFV; PAULO SÁVIO LOPES, UFV; JOSÉ DOMINGOS GUIMARÃES, UFV; SIMONE E. F. GUIMARÃES, UFV. |
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Título: |
Expression profile of genes associated with mastitis in dairy cattle. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, Ribeirão Preto, v. 32, n. 4, p. 776-781, 2009. |
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DOI: |
https://doi.org/10.1590/S1415-47572009005000074 |
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Idioma: |
Inglês |
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Conteúdo: |
In order to characterize the expression of genes associated with immune response mechanisms to mastitis, we quantified the relative expression of the IL-2, IL-4, IL-6, IL-8, IL-10, IFN-γ and TNF-α genes in milk cells of healthy cows and cows with clinical mastitis. Total RNA was extracted from milk cells of six Black and White Holstein (BW) cows and six Gyr cows, including three animals with and three without mastitis per breed. Gene expression was analyzed by real-time PCR. IL-10 gene expression was higher in the group of BW and Gyr cows with mastitis compared to animals free of infection from both breeds (p < 0.05). It was also higher in BW Holstein animals with clinical mastitis (p < 0.001), but it was not significant when Gyr cows with and without mastitis were compared (0.05 < p < 0.10). Among healthy cows, BW Holstein animals tended to present a higher expression of all genes studied, with a significant difference for the IL-2 and IFN-γ genes (p < 0.001). For animals with mastitis no significant difference in gene expression was observed between the two breeds. These findings suggest that animals with mastitis develop a preferentially cell-mediated immune response. Further studies including larger samples are necessary to better characterize the gene expression profile in cows with mastitis. |
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Palavras-Chave: |
Cytokine; Real-time PCR. |
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Thesaurus Nal: |
gene expression; immune response. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/23937/1/Lange-2009-GenMolBiol.pdf
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Marc: |
LEADER 02177naa a2200277 a 4500
001 1697368
005 2024-02-05
008 2009 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/S1415-47572009005000074$2DOI
100 1 $aFONSECA, I.
245 $aExpression profile of genes associated with mastitis in dairy cattle.$h[electronic resource]
260 $c2009
520 $aIn order to characterize the expression of genes associated with immune response mechanisms to mastitis, we quantified the relative expression of the IL-2, IL-4, IL-6, IL-8, IL-10, IFN-γ and TNF-α genes in milk cells of healthy cows and cows with clinical mastitis. Total RNA was extracted from milk cells of six Black and White Holstein (BW) cows and six Gyr cows, including three animals with and three without mastitis per breed. Gene expression was analyzed by real-time PCR. IL-10 gene expression was higher in the group of BW and Gyr cows with mastitis compared to animals free of infection from both breeds (p < 0.05). It was also higher in BW Holstein animals with clinical mastitis (p < 0.001), but it was not significant when Gyr cows with and without mastitis were compared (0.05 < p < 0.10). Among healthy cows, BW Holstein animals tended to present a higher expression of all genes studied, with a significant difference for the IL-2 and IFN-γ genes (p < 0.001). For animals with mastitis no significant difference in gene expression was observed between the two breeds. These findings suggest that animals with mastitis develop a preferentially cell-mediated immune response. Further studies including larger samples are necessary to better characterize the gene expression profile in cows with mastitis.
650 $agene expression
650 $aimmune response
653 $aCytokine
653 $aReal-time PCR
700 1 $aSILVA, P. V.
700 1 $aLANGE, C. C.
700 1 $aGUIMARAES, M. F. M.
700 1 $aWELLER, M. M. D. C. A.
700 1 $aSOUSA, K. R. S.
700 1 $aLOPES, P. S.
700 1 $aGUIMARÃES, J. D.
700 1 $aGUIMARÃES, S. E. F.
773 $tGenetics and Molecular Biology, Ribeirão Preto$gv. 32, n. 4, p. 776-781, 2009.
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Embrapa Gado de Leite (CNPGL) |
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| 1. |  | FONSECA, I.; SILVA, P. V.; LANGE, C. C.; GUIMARAES, M. F. M.; WELLER, M. M. D. C. A.; SOUSA, K. R. S.; LOPES, P. S.; GUIMARÃES, J. D.; GUIMARÃES, S. E. F. Expression profile of genes associated with mastitis in dairy cattle. Genetics and Molecular Biology, Ribeirão Preto, v. 32, n. 4, p. 776-781, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
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