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Biblioteca(s):  Embrapa Mandioca e Fruticultura.
Data corrente:  20/04/2004
Data da última atualização:  20/04/2004
Autoria:  SANTANA, M. S.; MATHEUS, J.
Título:  In situ inmunofluorescent localization of linamarase in cassava (Manihot esculenta Crantz).
Ano de publicação:  1998
Fonte/Imprenta:  Revista Brasileira de Mandioca, Salvador, v.17, p.76, nov., 1998. Suplemento.
Idioma:  Inglês
Conteúdo:  Abstract: Cassava (Manihot esculenta Crantz) is a cianogenic species consumed as a major staple food in many tropical countries. Cianogenic glucosides are synthesized in leaf tissues tranported to starch roots where they are stored. Mechanical demage of cassava tissues leads to the release of hydrocyanic acid (HCN), a volatile highly toxic-compound. Under normal physiological conditions cassava tissues contains no detectable HCN since the cianogenic glucosides and its degrading enzymes, linamarase and hydroxynitrile lyase, exists in different in compartments. Compartmentation of linamarase has been studied previously by other authors in several species measuring enzyme activity in different tissues. In leaves, linamarase is reported to be entirely apoplastic and associated to the cell walls. However, Pancoro and Hunghes (1992) detected the expression of mRNA and the activity of linamarase only in laticifers of cassava leaves. The present study investigates the location of linamarase in leaves and roots of two varieties of cassava of low and high content of cianoglucosides by inmunoflurescent labelling. Anti-linamarase immure e serum was obtained by inoculating a rabbit with purified linamarase. Hand-cults tissues were incubated with the anti-linamarase serum and then incubated with a commercial FITC-anti-rabbit IgG conjugate. Pre-inmune serum was control. The results shows the inmunoflurescent label distributed mainly in laticifers of leaves and roots in both varieties with ... Mostrar Tudo
Categoria do assunto:  --
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Registro original:  Embrapa Mandioca e Fruticultura (CNPMF)
Biblioteca ID Origem Tipo/Formato Classificação Cutter Registro Volume Status URL
CNPMF20997 - 1ADDAP - --633.68205
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Biblioteca(s):  Embrapa Agrobiologia.
Data corrente:  14/05/2004
Data da última atualização:  14/05/2004
Autoria:  DECLERCK, S.; D'OR, D.; BIVORT, C.; SOUZA, F. A. de.
Título:  Development of extraradical mycelium of Scutellopora reticulata under root-organ culture: spore production and function of auxiliary cells.
Ano de publicação:  2004
Fonte/Imprenta:  Mycological Research, New York, v.108. n. 1, p. 84-92, jan. 2004.
Idioma:  Inglês
Conteúdo:  The development of the extraradical mycelium and auxiliary cells and spore production of Scutellospora reticulata in association with Ri-DNA transformed carrot was followed under root-organ culture conditions. Extraradical mycelium development followed classical lag-exponential-plateau phases, with an additional late decline phase en number of auxiliary cells. Spore production started in parallel with a critical extraradical mycelium biomass produced, continued long after root growth ceased and during the late decline in auxiliary cells were shown to exhibit hyphal re-growth, but not root colonization, either in situ or in vitro. These results showed that root and extraradical mycelium development were intimately associated in a sequence where both grew together during active root growth, followed during root aging by a period in which only the fungus developed. Spore production appeared dependent on a critical extraradical mycelium biomass on the re-allocation of resources from both the intraradical mycelium and the auxiliary cells via the hyphal network.
Palavras-Chave:  Scutellospora reticulata.
Categoria do assunto:  --
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Registro original:  Embrapa Agrobiologia (CNPAB)
Biblioteca ID Origem Tipo/Formato Classificação Cutter Registro Volume Status
CNPAB29872 - 1UMTSP - --22708
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