| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Semiárido; Embrapa Uva e Vinho. |
|
Data corrente: |
03/03/2005 |
|
Data da última atualização: |
02/07/2015 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
DIANESE, E. de C.; RAMALHO, E. D.; CERQUEIRA, D. M.; LOPES, D. B.; FAJARDO, T. V. M.; FERREIRA, M. A. S. V.; MARTINS, C. R. F. |
|
Afiliação: |
Thor Vinicius Martins Fajardo, Embrapa Uva e Vinho; Érico de C. Dianese, Unb; Eduardo D. Ramalho, UnB; Daniela M. Cerqueira, UnB; Daniela B. Lopes, Embrapa Semi-Árido; Marisa A. S. V. Ferreira, UnB; Cláudia R. F. Martins, UnB. |
|
Título: |
Variability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil. |
|
Ano de publicação: |
2005 |
|
Fonte/Imprenta: |
Fitopatologia Brasileira, Brasília, v. 30, n. 2, p. 173-176, mar./abr. 2005. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Many viral diseases, including leafroll, which is of great economic importance, affect grapevines (Vitis spp.). A complex of eight viruses [Grapevine leafroll-associated virus (GLRaV) -1 to 8] is associated with this disease. The objective of this study was to compare the variability of the 3? terminal region of the polymerase gene of three isolates of GLRaV-3 (Grapevine leafroll-associated virus-3), from Submédio do Vale do Rio São Francisco (Petrolina-PE) with that of other isolates available at the GenBank, including an isolate from North America and another from Southern Brazil. The viral RNA was extracted from three infected ELISA reactive plants and a fragment of 340 bp was amplified, by RT-PCR, using primers that recognize that portion of the polymerase gene found between nucleotides 8267 and 8606. The three isolates from Vale do Rio São Francisco named Pet-1, Pet-2 and Pet-3, showed similarities ranging from 98% and 94%, respectively to the isolates from North America (AF037268) and Southern Brazilian (AF438411). Considering the whole genome, the main variation found was one amino acid change at position 2766 (F2766Y). These preliminary data indicate the existence of a natural variation among GLRaV-3 isolates from grapevines. This could be due to the vegetative propagation and long cycle of the plant, associated with the error-prone nature of RNA-dependent RNA polymerase. |
|
Palavras-Chave: |
Brasil; GLRa-3; Polimerase; Vale do São francisco; Videira; Vitis spp. |
|
Thesagro: |
Doença; Doença de Planta; RNA; Uva; Vírus. |
|
Thesaurus Nal: |
Ampelovirus. |
|
Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
|
URL: |
https://www.scielo.br/pdf/fb/v30n2/a12v30n2.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/183986/1/Daniela.pdf
|
|
Marc: |
LEADER 02360naa a2200337 a 4500
001 1543909
005 2015-07-02
008 2005 bl uuuu u00u1 u #d
100 1 $aDIANESE, E. de C.
245 $aVariability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil.
260 $c2005
520 $aMany viral diseases, including leafroll, which is of great economic importance, affect grapevines (Vitis spp.). A complex of eight viruses [Grapevine leafroll-associated virus (GLRaV) -1 to 8] is associated with this disease. The objective of this study was to compare the variability of the 3? terminal region of the polymerase gene of three isolates of GLRaV-3 (Grapevine leafroll-associated virus-3), from Submédio do Vale do Rio São Francisco (Petrolina-PE) with that of other isolates available at the GenBank, including an isolate from North America and another from Southern Brazil. The viral RNA was extracted from three infected ELISA reactive plants and a fragment of 340 bp was amplified, by RT-PCR, using primers that recognize that portion of the polymerase gene found between nucleotides 8267 and 8606. The three isolates from Vale do Rio São Francisco named Pet-1, Pet-2 and Pet-3, showed similarities ranging from 98% and 94%, respectively to the isolates from North America (AF037268) and Southern Brazilian (AF438411). Considering the whole genome, the main variation found was one amino acid change at position 2766 (F2766Y). These preliminary data indicate the existence of a natural variation among GLRaV-3 isolates from grapevines. This could be due to the vegetative propagation and long cycle of the plant, associated with the error-prone nature of RNA-dependent RNA polymerase.
650 $aAmpelovirus
650 $aDoença
650 $aDoença de Planta
650 $aRNA
650 $aUva
650 $aVírus
653 $aBrasil
653 $aGLRa-3
653 $aPolimerase
653 $aVale do São francisco
653 $aVideira
653 $aVitis spp
700 1 $aRAMALHO, E. D.
700 1 $aCERQUEIRA, D. M.
700 1 $aLOPES, D. B.
700 1 $aFAJARDO, T. V. M.
700 1 $aFERREIRA, M. A. S. V.
700 1 $aMARTINS, C. R. F.
773 $tFitopatologia Brasileira, Brasília$gv. 30, n. 2, p. 173-176, mar./abr. 2005.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Uva e Vinho (CNPUV) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 1 | |
| 1. |  | DIANESE, E. de C.; RAMALHO, E. D.; CERQUEIRA, D. M.; LOPES, D. B.; FAJARDO, T. V. M.; FERREIRA, M. A. S. V.; MARTINS, C. R. F. Variability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil. Fitopatologia Brasileira, Brasília, v. 30, n. 2, p. 173-176, mar./abr. 2005.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
| Biblioteca(s): Embrapa Semiárido; Embrapa Uva e Vinho. |
|    |
| Registros recuperados : 1 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
