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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
28/05/2003 |
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Data da última atualização: |
25/08/2023 |
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Autoria: |
ARITA, G. M. M.; GATTI, M. S. V.; DEAK, J. G.; PESTANA DE CASTRO, A. F. |
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Título: |
Bluetongue virus: production and study of viral antigen for serological diagnosis. |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
Journal of Virological Methods, v. 44, n. 2/3, p. 281-286, 1993. |
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DOI: |
https://doi.org/10.1016/0166-0934(93)90063-W |
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Idioma: |
Inglês |
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Conteúdo: |
A soluble antigen, produced from the culture supernatant of VERO cells infected with bluetongue virus serotype 4 (BTV-S4) and concentrated by sequential ultrafiltration with membranes with cut-off values 103 and 25 x 103 NMWP, showed complete identity to standard antigens when compared by agar gel immunodiffusion (AGID) and SDS-PAGE profiles, revealing that the main protein component responsible for the AGID reaction has a molecular weight of about 60 kDa corresponding probably to the NS1 protein. |
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Palavras-Chave: |
Imunodifusão; Soluble antigen; Sorotipo. |
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Thesagro: |
Anticorpo; Doença Animal; Língua Azul; Vírus. |
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Thesaurus Nal: |
Bluetongue virus; Immunodiffusion tests; Orbivirus; Polyacrylamide gel electrophoresis; Reoviridae; Sheep diseases. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 01466naa a2200325 a 4500
001 1529333
005 2023-08-25
008 1993 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/0166-0934(93)90063-W$2DOI
100 1 $aARITA, G. M. M.
245 $aBluetongue virus$bproduction and study of viral antigen for serological diagnosis.$h[electronic resource]
260 $c1993
520 $aA soluble antigen, produced from the culture supernatant of VERO cells infected with bluetongue virus serotype 4 (BTV-S4) and concentrated by sequential ultrafiltration with membranes with cut-off values 103 and 25 x 103 NMWP, showed complete identity to standard antigens when compared by agar gel immunodiffusion (AGID) and SDS-PAGE profiles, revealing that the main protein component responsible for the AGID reaction has a molecular weight of about 60 kDa corresponding probably to the NS1 protein.
650 $aBluetongue virus
650 $aImmunodiffusion tests
650 $aOrbivirus
650 $aPolyacrylamide gel electrophoresis
650 $aReoviridae
650 $aSheep diseases
650 $aAnticorpo
650 $aDoença Animal
650 $aLíngua Azul
650 $aVírus
653 $aImunodifusão
653 $aSoluble antigen
653 $aSorotipo
700 1 $aGATTI, M. S. V.
700 1 $aDEAK, J. G.
700 1 $aPESTANA DE CASTRO, A. F.
773 $tJournal of Virological Methods$gv. 44, n. 2/3, p. 281-286, 1993.
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Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
11/05/2016 |
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Data da última atualização: |
10/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
POMARI-FERNANDES, A.; BUENO, A. de F.; BORTOLI, S. A. de. |
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Afiliação: |
ALINE POMARI-FERNANDES, Universidade Federal da Fronteira Sul; ADENEY DE FREITAS BUENO, CNPSO; SÉRGIO ANTONIO DE BORTOLI, UNESP Jaboticabal. |
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Título: |
Size and flight ability of Telenomus remus parasitoids reared on eggs of the factitious host Corcyra cephalonica. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Revista Brasileira de Entomologia, v. 60, n. 2, p. 177-181, Apr./ Jun. 2016. |
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ISSN: |
1806-9665 |
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DOI: |
10.1016/j.rbe.2016.02.004 |
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Idioma: |
Português |
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Conteúdo: |
In two independent bioassays, size and flight ability of parasitoids reared on eggs of Corcyra cephalonica for 19 generations and parasitoids reared on a natural host (Spodoptera frugiperda eggs) for 250 generations were compared as fast quality control procedures for insect rearing. The size of parasitoids was examined by morphometric analysis using a stereoscope. Length and width of the wings, right hind tibia, and the body of 20 individuals (males and females) were measured. In the analysis of flight ability, parasitoids were divided into three groups: individuals able to fly ("flyers"), individuals that did not fly but had no visible deformation (?walkers?), and individuals with visible deformation ("deformed"). We observed that parasitoids were larger when reared on the natural host than on the factitious host for all evaluated morphological characters. However, there was no significant difference between the treatments regarding the number of "flyers", "walkers" or "deformed" parasitoids. This indicates that even though the rearing of T. remus on a factitious host affects parasitoid size, it does not necessarily affect its flight ability and therefore suggests that C. cephalonica is suitable as a factitious host for mass rearing of T. remus. Other biological parameters still need to be evaluated, such as host finding ability, parasitism capacity, and parasitoid field efficacy in order to provide a more complete picture of the effects caused by a host change. However, because fast laboratory tests are needed in rearing facilities, the one used in this study might be useful to rapidly assess parasitoid quality. MenosIn two independent bioassays, size and flight ability of parasitoids reared on eggs of Corcyra cephalonica for 19 generations and parasitoids reared on a natural host (Spodoptera frugiperda eggs) for 250 generations were compared as fast quality control procedures for insect rearing. The size of parasitoids was examined by morphometric analysis using a stereoscope. Length and width of the wings, right hind tibia, and the body of 20 individuals (males and females) were measured. In the analysis of flight ability, parasitoids were divided into three groups: individuals able to fly ("flyers"), individuals that did not fly but had no visible deformation (?walkers?), and individuals with visible deformation ("deformed"). We observed that parasitoids were larger when reared on the natural host than on the factitious host for all evaluated morphological characters. However, there was no significant difference between the treatments regarding the number of "flyers", "walkers" or "deformed" parasitoids. This indicates that even though the rearing of T. remus on a factitious host affects parasitoid size, it does not necessarily affect its flight ability and therefore suggests that C. cephalonica is suitable as a factitious host for mass rearing of T. remus. Other biological parameters still need to be evaluated, such as host finding ability, parasitism capacity, and parasitoid field efficacy in order to provide a more complete picture of the effects caused by a host change. However, b... Mostrar Tudo |
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Thesagro: |
Criação massal; Inimigo natural. |
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Thesaurus NAL: |
Insect rearing; Natural enemies. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/142882/1/Size-and-flight-ability....pdf
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Marc: |
LEADER 02352naa a2200217 a 4500
001 2044783
005 2017-05-10
008 2016 bl uuuu u00u1 u #d
022 $a1806-9665
024 7 $a10.1016/j.rbe.2016.02.004$2DOI
100 1 $aPOMARI-FERNANDES, A.
245 $aSize and flight ability of Telenomus remus parasitoids reared on eggs of the factitious host Corcyra cephalonica.$h[electronic resource]
260 $c2016
520 $aIn two independent bioassays, size and flight ability of parasitoids reared on eggs of Corcyra cephalonica for 19 generations and parasitoids reared on a natural host (Spodoptera frugiperda eggs) for 250 generations were compared as fast quality control procedures for insect rearing. The size of parasitoids was examined by morphometric analysis using a stereoscope. Length and width of the wings, right hind tibia, and the body of 20 individuals (males and females) were measured. In the analysis of flight ability, parasitoids were divided into three groups: individuals able to fly ("flyers"), individuals that did not fly but had no visible deformation (?walkers?), and individuals with visible deformation ("deformed"). We observed that parasitoids were larger when reared on the natural host than on the factitious host for all evaluated morphological characters. However, there was no significant difference between the treatments regarding the number of "flyers", "walkers" or "deformed" parasitoids. This indicates that even though the rearing of T. remus on a factitious host affects parasitoid size, it does not necessarily affect its flight ability and therefore suggests that C. cephalonica is suitable as a factitious host for mass rearing of T. remus. Other biological parameters still need to be evaluated, such as host finding ability, parasitism capacity, and parasitoid field efficacy in order to provide a more complete picture of the effects caused by a host change. However, because fast laboratory tests are needed in rearing facilities, the one used in this study might be useful to rapidly assess parasitoid quality.
650 $aInsect rearing
650 $aNatural enemies
650 $aCriação massal
650 $aInimigo natural
700 1 $aBUENO, A. de F.
700 1 $aBORTOLI, S. A. de
773 $tRevista Brasileira de Entomologia$gv. 60, n. 2, p. 177-181, Apr./ Jun. 2016.
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