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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
22/11/2002 |
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Data da última atualização: |
22/11/2002 |
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Autoria: |
TURZILLO, A. M.; CLAPPER, J. A.; MOSS, G. E.; NETT, T. M. |
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Título: |
Regulation of ovine GnRH receptor gene expression by progesterone and oestradiol. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Journal of Reproduction and Fertility, v. 113, n. 2, p. 251-256, 1998. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to determine whether progesterone prevents the stimulatory effects of oestradiol on GnRH receptor gene expression. In Expt 1, ewes were treated during the luteal phase (days 10-12 of the oestrous cycle) with either one or five subcutaneous implants containing oestradiol (n = 6 per group). Control ewes received no I treatment (n = 6). Anterior pituitary glands were collected 16 h after treatment with i oestradiol. Steady-state amounts of GnRH receptor mRNA were similar among all three I treatment groups despite increased circulating concentrations of oestradiol implanted ewes at the time of pituitary collection (4.3 +- 0.6 and 24.7 +- 2.6 pg ml-1 in ewes treated , with one or five implants, respectively, compared with 0.5 pg ml-1 in controls). Experiment 2 was designed to determine whether progesterone was the ovarian factor preventing the I stimulatory effects of oestradiol on. expression of the GnRH receptor gene in Expt 1. Twenty-five ewes were ovariectomized on day 6 or day 7 of the oestrous cycle and assigned to one of five treatment groups (n = 5 per group). Control ewes received no further treatment. Endogenous luteal phase concentrations of progesterone were replaced in three groups of ewes at the time of ovariectomy via intravaginal implants. Three days after ovariectomy, one group of progesterone-treated ewes received one oestradiol implant, while another group of progesterone-treated ewes received five oestradiol implants. An additional group was treated with five oestradiol implants only, and anterior pituitary glands were collected from alI ewes 16 h later. Compared with untreated ovariectomized ewes, treatment with progesterone alone did not affect amounts of GnRH receptor mRNA. In ewes treated with progesterone and either one or five oestradiol implants, steady-state amounts of GnRH receptor mRNA were increased twofold (P < 0.01). Treatment with oestradiol in the absence of progesterone increased amounts of GnRH receptor mRNA threefold (P < 0.001). These results provide evidence that the stimulatory effects of oestradiol on the expression of the GnRH receptor gene are prevented during the natural luteal phase in ewes. However, progesterone does not appear to act independently to mediate this effect. MenosThe objective of this study was to determine whether progesterone prevents the stimulatory effects of oestradiol on GnRH receptor gene expression. In Expt 1, ewes were treated during the luteal phase (days 10-12 of the oestrous cycle) with either one or five subcutaneous implants containing oestradiol (n = 6 per group). Control ewes received no I treatment (n = 6). Anterior pituitary glands were collected 16 h after treatment with i oestradiol. Steady-state amounts of GnRH receptor mRNA were similar among all three I treatment groups despite increased circulating concentrations of oestradiol implanted ewes at the time of pituitary collection (4.3 +- 0.6 and 24.7 +- 2.6 pg ml-1 in ewes treated , with one or five implants, respectively, compared with 0.5 pg ml-1 in controls). Experiment 2 was designed to determine whether progesterone was the ovarian factor preventing the I stimulatory effects of oestradiol on. expression of the GnRH receptor gene in Expt 1. Twenty-five ewes were ovariectomized on day 6 or day 7 of the oestrous cycle and assigned to one of five treatment groups (n = 5 per group). Control ewes received no further treatment. Endogenous luteal phase concentrations of progesterone were replaced in three groups of ewes at the time of ovariectomy via intravaginal implants. Three days after ovariectomy, one group of progesterone-treated ewes received one oestradiol implant, while another group of progesterone-treated ewes received five oestradiol implants. An additio... Mostrar Tudo |
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Palavras-Chave: |
GnRH; Receptor. |
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Thesagro: |
Endocrinologia; Estradiol; Hormônio; Ovino; Progesterona. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02932naa a2200241 a 4500
001 1528866
005 2002-11-22
008 1998 bl uuuu u00u1 u #d
100 1 $aTURZILLO, A. M.
245 $aRegulation of ovine GnRH receptor gene expression by progesterone and oestradiol.
260 $c1998
520 $aThe objective of this study was to determine whether progesterone prevents the stimulatory effects of oestradiol on GnRH receptor gene expression. In Expt 1, ewes were treated during the luteal phase (days 10-12 of the oestrous cycle) with either one or five subcutaneous implants containing oestradiol (n = 6 per group). Control ewes received no I treatment (n = 6). Anterior pituitary glands were collected 16 h after treatment with i oestradiol. Steady-state amounts of GnRH receptor mRNA were similar among all three I treatment groups despite increased circulating concentrations of oestradiol implanted ewes at the time of pituitary collection (4.3 +- 0.6 and 24.7 +- 2.6 pg ml-1 in ewes treated , with one or five implants, respectively, compared with 0.5 pg ml-1 in controls). Experiment 2 was designed to determine whether progesterone was the ovarian factor preventing the I stimulatory effects of oestradiol on. expression of the GnRH receptor gene in Expt 1. Twenty-five ewes were ovariectomized on day 6 or day 7 of the oestrous cycle and assigned to one of five treatment groups (n = 5 per group). Control ewes received no further treatment. Endogenous luteal phase concentrations of progesterone were replaced in three groups of ewes at the time of ovariectomy via intravaginal implants. Three days after ovariectomy, one group of progesterone-treated ewes received one oestradiol implant, while another group of progesterone-treated ewes received five oestradiol implants. An additional group was treated with five oestradiol implants only, and anterior pituitary glands were collected from alI ewes 16 h later. Compared with untreated ovariectomized ewes, treatment with progesterone alone did not affect amounts of GnRH receptor mRNA. In ewes treated with progesterone and either one or five oestradiol implants, steady-state amounts of GnRH receptor mRNA were increased twofold (P < 0.01). Treatment with oestradiol in the absence of progesterone increased amounts of GnRH receptor mRNA threefold (P < 0.001). These results provide evidence that the stimulatory effects of oestradiol on the expression of the GnRH receptor gene are prevented during the natural luteal phase in ewes. However, progesterone does not appear to act independently to mediate this effect.
650 $aEndocrinologia
650 $aEstradiol
650 $aHormônio
650 $aOvino
650 $aProgesterona
653 $aGnRH
653 $aReceptor
700 1 $aCLAPPER, J. A.
700 1 $aMOSS, G. E.
700 1 $aNETT, T. M.
773 $tJournal of Reproduction and Fertility$gv. 113, n. 2, p. 251-256, 1998.
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Embrapa Caprinos e Ovinos (CNPC) |
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| 4. |  | CALBO, A. G.; VAZ, C. M. P.; RABELLO, L. M. Controle atmomagnético de irrigação com sensores IG. In: SIMPÓSIO NACIONAL DE INSTRUMENTAÇÃO AGROPECUÁRIA, 2014, São Carlos, SP Anais do SIAGRO: ciência, inovação e mercado 2014. São Carlos, SP: Embrapa Instrumentação, 2014. p. 337-340. Editores: Carlos Manoel Pedro Vaz, Débora Marcondes Bastos Pereira Milori, Silvio Crestana.| Tipo: Artigo em Anais de Congresso |
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| 6. | | PRIMAVESI, A. C. P. de A.; GODOY, R.; PRIMAVESI, O. M. A. S. P. R.; SOUZA, F. H. D. de. Ensaio nacional de aveias forrageiras para cobertura de solos. São Carlos, SP, 2003. In: REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE AVEIA, 24., 2004, Pelotas. Resultados experimentais... Pelotas: UFPelotas, 2004. p.310-311.| Tipo: Resumo em Anais de Congresso |
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| 8. | | IGUMA, L. T.; FERREIRA, A. de M.; SÁ, W. F. de; VIANA, J. H. M.; CAMARGO, L. S. de A. Manejo reproductivo de rebanõs lecheros. In: MARTINS, P. do C.; DINIZ, F. H.; MOREIRA, M. S. de P.; NOGUEIRA NETTO, V.; ARCURI, P. B. (Ed.). Conocimientos y estratégias tecnológicas para la producción de leche en regiones tropicales. Juiz de Fora: Embrapa Gado de Leite, 2007. p. 289-307.| Tipo: Capítulo em Livro Técnico-Científico | Circulação/Nível: -- - -- |
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| 10. | | FIORENTINI, A. M.; FREIRE, V. A. P.; SILVA, W. P.; PINHEIRO, M. S.; GONÇALVES, M. S.; ARNONI, R. K.; POUEY, J. L. O. F.; LEMES, J. S.; ESTEVES, R. M. G. Qualidade microbiológica e sensorial de hambúrgueres elaborados com carne de capivara (Hydrochaeris hydrochaeris) na região de Pelotas/Brasil. In: CONGRESO ARGENTINO DE CIENCIA Y TECNOLOGÍA DE ALIMENTOS, 13., Buenos Aires, 2011. Anais... Buenos Aires: Asociación Argentina de Tecnólogos Alimentarios, 2011. 1 CD ROM.| Tipo: Artigo em Anais de Congresso |
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| 11. | | PESSOA, M. C. P. Y.; FRASCA-SCORVO, C. M. D.; LOSEKANN, M. E.; SILVA, M. S. G. M. e; SILVA, C. A. da; HISANO, H.; SCORVO FILHO, J. D.; INOUE, L. A. K. A. Método para promover a inclusão digital de produtores de tilápia pelo uso do Aquisys v. 1.3. Jaguariúna: Embrapa Meio Ambiente, 2016. 52 p. (Embrapa Meio Ambiente. Documentos, 104).| Biblioteca(s): Embrapa Agropecuária Oeste; Embrapa Meio Ambiente. |
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