BDPA - Bases de Dados da Pesquisa Agropecuária Embrapa
 






Registro Completo
Biblioteca(s):  Embrapa Caprinos e Ovinos.
Data corrente:  31/10/2002
Data da última atualização:  31/10/2002
Autoria:  GENTRY, P. C.; SMITH, G. W.; LEIGHR, D. R.; BAO, B.; SMITH, M. F.
Título:  Ontogeny of stem cell factor receptor (c-kit) messenger ribonucleic acid in the ovine corpus luteum.
Ano de publicação:  1998
Fonte/Imprenta:  Biology of Reproduction, v. 59, n. 4, p. 983-990, 1998.
Idioma:  Inglês
Conteúdo:  Stem cell factor (SCF) is a pleiotropic growth factor that is expressed by the ovine corpus luteum throughout its life span by both small and large steroidogenic cells. Determination of the action of SCF, however, requires localization of its receptor, c-kit; therefore, the objectives of the present study were to identify and localize c-kit within corpora lutea. Two cDNAs encoding different portions of the c-kit molecule were amplified by the polymerase chain reaction. The first was a 558-base pair (bp) cDNA encoding portions of the transmembrane and tyrosine kinase domains; the second was a 632-bp cDNA encoding most of the ligand-binding domain. Expression of c-kit was quantified by RNase protection assay of total cellular RNA collected on Days 3, 7, 10, 13, and 16 (n = 4,4, 5, 4, and 4 per group, respectively) of the estrous cycle (Day O = estrus). The level of c-kit mRNA was low early in the luteal phase, reached (p < 0.05) maximum levels on Day 13, and then decreased (p < 0.01) on Day 16. On Day 3 (n = 4), c-kit was expressed in a cell specific manner throughout the corpus luteum; identity of the specific cell types expressing c-kit could not be determined at this stage. On Day 14 (n = 4), c-kit did not appear to be expressed within large luteal cells but was prominently expressed in cells that surrounded large luteal cells and that possessed the morphological characteristics of small luteal cells and endothelial cells. Given the temporal regulation of c-kit expression w... Mostrar Tudo
Palavras-Chave:  Desenvolvimento biológico; Ontogenia; Receptor.
Thesagro:  Corpo Lúteo; Endocrinologia; Hormônio; Ovino; Reprodução Animal.
Categoria do assunto:  --
Marc:  Mostrar Marc Completo
Registro original:  Embrapa Caprinos e Ovinos (CNPC)
Biblioteca ID Origem Tipo/Formato Classificação Cutter Registro Volume Status URL
CNPC15065 - 1ADCAP - --v. 59(4)
Voltar
Nenhum registro encontrado para a expressão de busca informada.
Nenhum registro encontrado para a expressão de busca informada.
 
 

Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área Restrita

Embrapa Informática Agropecuária
Av. André Tosello, 209 - Barão Geraldo
Caixa Postal 6041- 13083-886 - Campinas, SP
PABX: SAC (19) 3211-5743
SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional