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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
26/06/2000 |
Data da última atualização: |
09/06/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BARDUCHE, D.; PAIVA, R.; LOPES, M. A.; PAIVA, E. |
Afiliação: |
EMBRAPA-CNPMS. |
Título: |
Effect of ABA and GA3 on protein mobilization in embryos and Cotyledons of angico (Anadenanthera peregrina (L.) sepg) seeds during germination. |
Ano de publicação: |
1999 |
Fonte/Imprenta: |
Brazilian Archives of Biology and Technology, Curitiba, v. 42, n. 2, p. 135-144, 1999. |
Idioma: |
Inglês |
Conteúdo: |
In this work, a woody species [A. peregrina (L.) Speg.] was studied in order to observe the effect of ABA and GA3 at the biochemical level during the process of seed germination. Embryos incubated in sucrose solution containing ABA and/or GA3 were analyzed through SDS-PAGE to observe the mobilization pattern of storage proteins during the beginning of germination. Cotyledons isolated from seeds incubated in aqueous solutions containing ABA and/or GA3, were also analyzed through SDS-PAGE and by PAGE/Activity Gels (polyacrylamide gels copolymerized with substrate for enzymes) to observe the mobilization pattern of storage proteins and protease activity after the beginning of the germination. Results of these experiments show that ABA blocks protein mobilization by inhibiting protease activity in cotyledons. This inhibition is not sufficient to prevent germination showing that the effect of ABA on germination is not dependent on protease activity. The blockage of storage protein mobilization was also observed in embryos, but no protease activity inhibition was clearly detected. ABA was able to induce the synthesis of proteins in cotyledons but not in embryos. A polypeptide with an approximate molecular weight of 17 kD, was degraded within 6 hours in control embryos, but this degradation was blocked by ABA and GA3. Using the same concentrations of ABA and GA3 on embryos and cotyledons, the effect of ABA was counteracted by GA3 in embryos, but not in cotyledons. Although the effects of ABA and GA3 were not so different from those shown in the literature, the behavior of 17 kD-polypeptide contradicts these reports suggesting that specific studies should be performed. MenosIn this work, a woody species [A. peregrina (L.) Speg.] was studied in order to observe the effect of ABA and GA3 at the biochemical level during the process of seed germination. Embryos incubated in sucrose solution containing ABA and/or GA3 were analyzed through SDS-PAGE to observe the mobilization pattern of storage proteins during the beginning of germination. Cotyledons isolated from seeds incubated in aqueous solutions containing ABA and/or GA3, were also analyzed through SDS-PAGE and by PAGE/Activity Gels (polyacrylamide gels copolymerized with substrate for enzymes) to observe the mobilization pattern of storage proteins and protease activity after the beginning of the germination. Results of these experiments show that ABA blocks protein mobilization by inhibiting protease activity in cotyledons. This inhibition is not sufficient to prevent germination showing that the effect of ABA on germination is not dependent on protease activity. The blockage of storage protein mobilization was also observed in embryos, but no protease activity inhibition was clearly detected. ABA was able to induce the synthesis of proteins in cotyledons but not in embryos. A polypeptide with an approximate molecular weight of 17 kD, was degraded within 6 hours in control embryos, but this degradation was blocked by ABA and GA3. Using the same concentrations of ABA and GA3 on embryos and cotyledons, the effect of ABA was counteracted by GA3 in embryos, but not in cotyledons. Although the effe... Mostrar Tudo |
Palavras-Chave: |
ABA; GA3; Mobilizacao; Mobilization; Protease activity; Protein; Tropical woody plant. |
Thesagro: |
Germinação; Proteína. |
Thesaurus Nal: |
germination. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/48211/1/Effect-ABA.pdf
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Marc: |
LEADER 02520naa a2200277 a 4500 001 1484074 005 2018-06-09 008 1999 bl uuuu u00u1 u #d 100 1 $aBARDUCHE, D. 245 $aEffect of ABA and GA3 on protein mobilization in embryos and Cotyledons of angico (Anadenanthera peregrina (L.) sepg) seeds during germination.$h[electronic resource] 260 $c1999 520 $aIn this work, a woody species [A. peregrina (L.) Speg.] was studied in order to observe the effect of ABA and GA3 at the biochemical level during the process of seed germination. Embryos incubated in sucrose solution containing ABA and/or GA3 were analyzed through SDS-PAGE to observe the mobilization pattern of storage proteins during the beginning of germination. Cotyledons isolated from seeds incubated in aqueous solutions containing ABA and/or GA3, were also analyzed through SDS-PAGE and by PAGE/Activity Gels (polyacrylamide gels copolymerized with substrate for enzymes) to observe the mobilization pattern of storage proteins and protease activity after the beginning of the germination. Results of these experiments show that ABA blocks protein mobilization by inhibiting protease activity in cotyledons. This inhibition is not sufficient to prevent germination showing that the effect of ABA on germination is not dependent on protease activity. The blockage of storage protein mobilization was also observed in embryos, but no protease activity inhibition was clearly detected. ABA was able to induce the synthesis of proteins in cotyledons but not in embryos. A polypeptide with an approximate molecular weight of 17 kD, was degraded within 6 hours in control embryos, but this degradation was blocked by ABA and GA3. Using the same concentrations of ABA and GA3 on embryos and cotyledons, the effect of ABA was counteracted by GA3 in embryos, but not in cotyledons. Although the effects of ABA and GA3 were not so different from those shown in the literature, the behavior of 17 kD-polypeptide contradicts these reports suggesting that specific studies should be performed. 650 $agermination 650 $aGerminação 650 $aProteína 653 $aABA 653 $aGA3 653 $aMobilizacao 653 $aMobilization 653 $aProtease activity 653 $aProtein 653 $aTropical woody plant 700 1 $aPAIVA, R. 700 1 $aLOPES, M. A. 700 1 $aPAIVA, E. 773 $tBrazilian Archives of Biology and Technology, Curitiba$gv. 42, n. 2, p. 135-144, 1999.
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Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
18/07/2011 |
Data da última atualização: |
26/05/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
REIS, R. C.; MINIM, V. P. R.; BOLINI, H. M. A.; DIAS, B. R. P.; MINIM, L. A.; CERESINO, E. B. |
Afiliação: |
RONIELLI CARDOSO REIS, CNPMF; Valéria Paula Rodrigues Minim, UFV; Helena Maria André Bolini, USP; Beatriz Rodrigues Pimentel Dias, UFV; Luis Antônio Minim, UFV; Elaine Berger Ceresino, UFV. |
Título: |
Sweetness equivalence of different sweeteners in strawberry-flavored yogurt. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Journal of Food Quality, Westport, v. 34, n. 3, p. 163-170, june, 2011. |
ISSN: |
1745-4557 |
DOI: |
10.1111/j.1745-4557.2011.00378.x |
Idioma: |
Inglês |
Conteúdo: |
To successfully substitute sucrose for sweeteners, further studiesmust be carried out based on previous knowledge of sweetener concentration to determine the equivalent sweetness of such compounds. In this work, sweetness equivalence of strawberry-flavored yogurt with different sweeteners and/or their combinations (aspartame, acesulfame-K, cyclamate, saccharin, stevia and sucralose) and yogurt sweetened with 11.5% w/w sucrose was measured using the sensory magnitude estimation method. The sweetness concentrations equivalent to strawberry yogurt sweetened with 11.5% w/w sucrose in the tested sweeteners were 0.072% w/w for aspartame, 0.042% w/w for aspartame/acesulfame-K (2:1), 0.064% w/w for cyclamate/saccharin, 0.043% w/w for cyclamate/saccharin (2:1)/stevia (1.8:1) and 0.30% w/w for sucralose. These results can promote the use of different sweetener combinations in strawberry-flavored yogurt, specially acesulfame-K and stevia, once they produce more pleasing in this product. |
Thesagro: |
Alimento Preparado; Tecnologia de Alimento. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 01693naa a2200229 a 4500 001 1896134 005 2023-05-26 008 2011 bl uuuu u00u1 u #d 022 $a1745-4557 024 7 $a10.1111/j.1745-4557.2011.00378.x$2DOI 100 1 $aREIS, R. C. 245 $aSweetness equivalence of different sweeteners in strawberry-flavored yogurt.$h[electronic resource] 260 $c2011 520 $aTo successfully substitute sucrose for sweeteners, further studiesmust be carried out based on previous knowledge of sweetener concentration to determine the equivalent sweetness of such compounds. In this work, sweetness equivalence of strawberry-flavored yogurt with different sweeteners and/or their combinations (aspartame, acesulfame-K, cyclamate, saccharin, stevia and sucralose) and yogurt sweetened with 11.5% w/w sucrose was measured using the sensory magnitude estimation method. The sweetness concentrations equivalent to strawberry yogurt sweetened with 11.5% w/w sucrose in the tested sweeteners were 0.072% w/w for aspartame, 0.042% w/w for aspartame/acesulfame-K (2:1), 0.064% w/w for cyclamate/saccharin, 0.043% w/w for cyclamate/saccharin (2:1)/stevia (1.8:1) and 0.30% w/w for sucralose. These results can promote the use of different sweetener combinations in strawberry-flavored yogurt, specially acesulfame-K and stevia, once they produce more pleasing in this product. 650 $aAlimento Preparado 650 $aTecnologia de Alimento 700 1 $aMINIM, V. P. R. 700 1 $aBOLINI, H. M. A. 700 1 $aDIAS, B. R. P. 700 1 $aMINIM, L. A. 700 1 $aCERESINO, E. B. 773 $tJournal of Food Quality, Westport$gv. 34, n. 3, p. 163-170, june, 2011.
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