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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
31/10/1994 |
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Data da última atualização: |
17/10/2023 |
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Autoria: |
LOPES, M. A. |
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Afiliação: |
EMBRAPA/CNPMS. |
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Título: |
Genetic and biochemical characterization of the maize mutants floury-2 and modified opaque-2. |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
Arizona: University of Arizona, 1993. |
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Páginas: |
269p. |
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Idioma: |
Inglês |
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Notas: |
Doctor Sc. Thesis. |
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Conteúdo: |
The maize mutants floury-2 (fl2) and opaque-2 (02) reduce endosperm storage protein synthesis and lead to accumulation of protein fractions that are nutritionally more balanced. However, this advantage is offset by the soft and fragile texture of the endosperm, which gives the grain inferior quality for harvesting, storage, and processing. Further development and future usefulness of these materials may depend upon deciphering the genetic., biochemical and molecular mechanisms that affet seed physical structure and nutritional quality. The soft endosperm of the maize floury-2 (fl2) mutant is associated with a reduction in zein mRNA and protein synthesis, and enhanced levels of a 70-kD protein shown to be the maize homolog of the chaperonin BIP. An unusual alpha-zein protein of 24-kD was found to be consistently associated with the storage protein fraction from fl2 mutants. Increases in accumulation of this novel alpha-zein protein is correlated with increases in BIP accumulation and decreases in storage protein deposition. Also, an rflp that is unique to fl2 was detected with a 22-DF alpha-zein probe. the synthesis of an abnormal alpha-zein protein in fl2 could explain many features of the mutant, such as abnormal protein body morphology, BIP induction and hypostasis to 02. These findings indicate that the effects of the fl2 mutation could be a response to accumulation of a defective protein that causes impairment of secretory protein synthesis. Breeding methods based on accumulation of 02 modifier genes led (...) MenosThe maize mutants floury-2 (fl2) and opaque-2 (02) reduce endosperm storage protein synthesis and lead to accumulation of protein fractions that are nutritionally more balanced. However, this advantage is offset by the soft and fragile texture of the endosperm, which gives the grain inferior quality for harvesting, storage, and processing. Further development and future usefulness of these materials may depend upon deciphering the genetic., biochemical and molecular mechanisms that affet seed physical structure and nutritional quality. The soft endosperm of the maize floury-2 (fl2) mutant is associated with a reduction in zein mRNA and protein synthesis, and enhanced levels of a 70-kD protein shown to be the maize homolog of the chaperonin BIP. An unusual alpha-zein protein of 24-kD was found to be consistently associated with the storage protein fraction from fl2 mutants. Increases in accumulation of this novel alpha-zein protein is correlated with increases in BIP accumulation and decreases in storage protein deposition. Also, an rflp that is unique to fl2 was detected with a 22-DF alpha-zein probe. the synthesis of an abnormal alpha-zein protein in fl2 could explain many features of the mutant, such as abnormal protein body morphology, BIP induction and hypostasis to 02. These findings indicate that the effects of the fl2 mutation could be a response to accumulation of a defective protein that causes impairment of secretory protein synthesis. Breeding methods based on acc... Mostrar Tudo |
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Palavras-Chave: |
Caracterizacao; Characterization; Flory-2; Genetic; Maize; Opaco-2; Opaque-2. |
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Thesagro: |
Biologia Molecular; Bioquímica; Biotecnologia; Genética; Melhoramento; Milho; Zea Mays. |
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Thesaurus Nal: |
biochemistry; biotechnology; breeding; corn; genetics; molecular biology. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02469nam a2200373 a 4500
001 1472701
005 2023-10-17
008 1993 bl uuuu m 00u1 u #d
100 1 $aLOPES, M. A.
245 $aGenetic and biochemical characterization of the maize mutants floury-2 and modified opaque-2.
260 $aArizona: University of Arizona$c1993
300 $a269p.
500 $aDoctor Sc. Thesis.
520 $aThe maize mutants floury-2 (fl2) and opaque-2 (02) reduce endosperm storage protein synthesis and lead to accumulation of protein fractions that are nutritionally more balanced. However, this advantage is offset by the soft and fragile texture of the endosperm, which gives the grain inferior quality for harvesting, storage, and processing. Further development and future usefulness of these materials may depend upon deciphering the genetic., biochemical and molecular mechanisms that affet seed physical structure and nutritional quality. The soft endosperm of the maize floury-2 (fl2) mutant is associated with a reduction in zein mRNA and protein synthesis, and enhanced levels of a 70-kD protein shown to be the maize homolog of the chaperonin BIP. An unusual alpha-zein protein of 24-kD was found to be consistently associated with the storage protein fraction from fl2 mutants. Increases in accumulation of this novel alpha-zein protein is correlated with increases in BIP accumulation and decreases in storage protein deposition. Also, an rflp that is unique to fl2 was detected with a 22-DF alpha-zein probe. the synthesis of an abnormal alpha-zein protein in fl2 could explain many features of the mutant, such as abnormal protein body morphology, BIP induction and hypostasis to 02. These findings indicate that the effects of the fl2 mutation could be a response to accumulation of a defective protein that causes impairment of secretory protein synthesis. Breeding methods based on accumulation of 02 modifier genes led (...)
650 $abiochemistry
650 $abiotechnology
650 $abreeding
650 $acorn
650 $agenetics
650 $amolecular biology
650 $aBiologia Molecular
650 $aBioquímica
650 $aBiotecnologia
650 $aGenética
650 $aMelhoramento
650 $aMilho
650 $aZea Mays
653 $aCaracterizacao
653 $aCharacterization
653 $aFlory-2
653 $aGenetic
653 $aMaize
653 $aOpaco-2
653 $aOpaque-2
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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