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Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
14/12/2005 |
Data da última atualização: |
04/09/2015 |
Autoria: |
ALMEIDA, A. M. R.; SAKAI, J.; OLIVEIRA, T. G. O.; BELINTANI, P.; KITAJIMA, E. W.; SOUTO, E. R.; NOVAES, T. G. de; NORA, P. S. |
Título: |
Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil. |
Ano de publicação: |
2005 |
Fonte/Imprenta: |
Fitopatologia Brasileira, Brasília, v. 30, n. 4, p. 366-373, July/Aug. 2005. |
Idioma: |
Inglês |
Conteúdo: |
A virus was isolated from soybean (Glycine max) plants with symptoms of dwarfing and bud blight in Wenceslau Braz County, Paraná, Brazil. The host range and properties resembled those of Tobacco streak virus (TSV). The purified virus showed three peaks in a frozen sucrose gradient. Antiserum was produced and the virus was serologically related to TSV. Electron microscopy detected 28 nm spherical particles. Coat protein (CP) had a Mr of 29.880 Da. A fragment of 1028 nt was amplified, cloned and sequenced. One open reading frame with 717 nt was identified and associated to the CP. The CP gene shared 83% identity with the sequence of TSV CP from white clover (Trifolium repens) (GenBank CAA25133). This is the first report of the biological and molecular characterization of TSV isolated from soybeans. It is proposed that this isolate be considered a strain of TSV named TSV-BR. |
Thesagro: |
Soja. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/129061/1/digitalizar0001.pdf
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Marc: |
LEADER 01537naa a2200217 a 4500 001 1468641 005 2015-09-04 008 2005 bl uuuu u00u1 u #d 100 1 $aALMEIDA, A. M. R. 245 $aBiological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil. 260 $c2005 520 $aA virus was isolated from soybean (Glycine max) plants with symptoms of dwarfing and bud blight in Wenceslau Braz County, Paraná, Brazil. The host range and properties resembled those of Tobacco streak virus (TSV). The purified virus showed three peaks in a frozen sucrose gradient. Antiserum was produced and the virus was serologically related to TSV. Electron microscopy detected 28 nm spherical particles. Coat protein (CP) had a Mr of 29.880 Da. A fragment of 1028 nt was amplified, cloned and sequenced. One open reading frame with 717 nt was identified and associated to the CP. The CP gene shared 83% identity with the sequence of TSV CP from white clover (Trifolium repens) (GenBank CAA25133). This is the first report of the biological and molecular characterization of TSV isolated from soybeans. It is proposed that this isolate be considered a strain of TSV named TSV-BR. 650 $aSoja 700 1 $aSAKAI, J. 700 1 $aOLIVEIRA, T. G. O. 700 1 $aBELINTANI, P. 700 1 $aKITAJIMA, E. W. 700 1 $aSOUTO, E. R. 700 1 $aNOVAES, T. G. de 700 1 $aNORA, P. S. 773 $tFitopatologia Brasileira, Brasília$gv. 30, n. 4, p. 366-373, July/Aug. 2005.
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Embrapa Soja (CNPSO) |
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1. | | MELO NETO, M. L. de; GONZAGA NETO, L.; BEZERRA, J. E. F.; LEDERMAN, I.; ASSIS, J. S. de. Efeito da poda e da cianamida hidrogenada associados à desfolha química e manual na indução floral de pinheiras. In: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 10.; CONGRESSO LATINO AMERICANO DE FISIOLOGIA VEGETAL, 12., 2005, Recife. Anais... Recife: SBFV, 2005. 1 CD-ROM.Tipo: Artigo em Anais de Congresso |
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