| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
21/03/2023 |
|
Data da última atualização: |
01/06/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
DOCEMA, M. L.; MORAES, T. DE S.; ATTÍLIO, L. B.; MARQUES, J. P. R.; STIPP, L. C. L.; HARAKAVA, R.; ASTUA, J. de F.; BELASQUE JUNIOR, J.; AZEVEDO, R. A.; GAZIOLA, S. A.; MOURÃO FILHO, F. DE A. A. |
|
Afiliação: |
MATHEUS LUÍS DOCEMA, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; TATIANA DE SOUZA MORAES, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; LÍSIA BORGES ATTÍLIO, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; JOÃO PAULO RODRIGUES MARQUES, UNIVERSIDADE DE SÃO PAULO; LILIANE CRISTINA LIBORIO STIPP, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; RICARDO HARAKAVA, INSTITUTO BIOLÓGICO; JULIANA DE FREITAS ASTUA, CNPMF; JOSÉ BELASQUE JUNIOR, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; RICARDO ANTUNES AZEVEDO, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; SALETE APARECIDA GAZIOLA, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; FRANCISCO DE ASSIS ALVES MOURÃO FILHO, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ. |
|
Título: |
Transgenic 'Hamlin' sweet orange expressing csd1 or d4e1 genes exhibits decreased susceptibility to citrus canker disease. |
|
Ano de publicação: |
2022 |
|
Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, 2022. |
|
DOI: |
https://doi.org/10.1007/s11240-022-02420-3 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Currently, there are no resistant sweet orange cultivars to the citrus canker (cc) [Xanthomonas citri subsp. citri (Xcc)], and the production of transgenic plants resistant to cc can be an efficient solution in the search of improved cultivars. Several strategies can be applied in this scenario, including the expression of genes that code for antimicrobial peptides and resistance genes from Xcc-tolerant cultivars. We evaluated the reaction of 'Hamlin' sweet orange (Citrus sinensis L. Osbeck) transformed with a gene that codes for the D4E1 synthetic antimicrobial peptide, and ?Hamlin? sweet orange plants transformed with the gene that codes for a copper and zinc superoxide dismutase (csd1), isolated from Poncirus trifoliata, after inoculation with Xcc. Transgenic plants were propagated through grafting in ?Rangpur? lime (Citrus limonia Osbeck), and then inoculated with a Xcc suspension. Hydrogen peroxide production, activity of selected antioxidant enzymes and callose deposition were evaluated in the transgenic plants subjected to Xcc inoculation. The incidence and the severity of citrus canker were significantly reduced in several transgenic events, which also exhibited lower Xcc titers when compared with non-transgenic plants. Transgenic events which exhibited higher superoxide dismutase activities also showed increased accumulation of hydrogen peroxide in foliar tissues after Xcc inoculation. Epifluorescence microscopy analyses revealed a high callose deposition in the sieve tube elements of leaf tissues in some transgenic events. Callose accumulation is an important plant defense response to biotic stresses and, in csd1 transgenic lines, it was associated with lower Xcc populations. MenosCurrently, there are no resistant sweet orange cultivars to the citrus canker (cc) [Xanthomonas citri subsp. citri (Xcc)], and the production of transgenic plants resistant to cc can be an efficient solution in the search of improved cultivars. Several strategies can be applied in this scenario, including the expression of genes that code for antimicrobial peptides and resistance genes from Xcc-tolerant cultivars. We evaluated the reaction of 'Hamlin' sweet orange (Citrus sinensis L. Osbeck) transformed with a gene that codes for the D4E1 synthetic antimicrobial peptide, and ?Hamlin? sweet orange plants transformed with the gene that codes for a copper and zinc superoxide dismutase (csd1), isolated from Poncirus trifoliata, after inoculation with Xcc. Transgenic plants were propagated through grafting in ?Rangpur? lime (Citrus limonia Osbeck), and then inoculated with a Xcc suspension. Hydrogen peroxide production, activity of selected antioxidant enzymes and callose deposition were evaluated in the transgenic plants subjected to Xcc inoculation. The incidence and the severity of citrus canker were significantly reduced in several transgenic events, which also exhibited lower Xcc titers when compared with non-transgenic plants. Transgenic events which exhibited higher superoxide dismutase activities also showed increased accumulation of hydrogen peroxide in foliar tissues after Xcc inoculation. Epifluorescence microscopy analyses revealed a high callose deposition in the sie... Mostrar Tudo |
|
Thesagro: |
Fruta Cítrica; Planta Transgênica. |
|
Thesaurus Nal: |
Antimicrobial peptides; Citrus; Superoxide dismutase; Transgenic plants; Xanthomonas citri subsp. citri. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
Marc: |
LEADER 02770naa a2200337 a 4500
001 2152545
005 2023-06-01
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11240-022-02420-3$2DOI
100 1 $aDOCEMA, M. L.
245 $aTransgenic 'Hamlin' sweet orange expressing csd1 or d4e1 genes exhibits decreased susceptibility to citrus canker disease.$h[electronic resource]
260 $c2022
520 $aCurrently, there are no resistant sweet orange cultivars to the citrus canker (cc) [Xanthomonas citri subsp. citri (Xcc)], and the production of transgenic plants resistant to cc can be an efficient solution in the search of improved cultivars. Several strategies can be applied in this scenario, including the expression of genes that code for antimicrobial peptides and resistance genes from Xcc-tolerant cultivars. We evaluated the reaction of 'Hamlin' sweet orange (Citrus sinensis L. Osbeck) transformed with a gene that codes for the D4E1 synthetic antimicrobial peptide, and ?Hamlin? sweet orange plants transformed with the gene that codes for a copper and zinc superoxide dismutase (csd1), isolated from Poncirus trifoliata, after inoculation with Xcc. Transgenic plants were propagated through grafting in ?Rangpur? lime (Citrus limonia Osbeck), and then inoculated with a Xcc suspension. Hydrogen peroxide production, activity of selected antioxidant enzymes and callose deposition were evaluated in the transgenic plants subjected to Xcc inoculation. The incidence and the severity of citrus canker were significantly reduced in several transgenic events, which also exhibited lower Xcc titers when compared with non-transgenic plants. Transgenic events which exhibited higher superoxide dismutase activities also showed increased accumulation of hydrogen peroxide in foliar tissues after Xcc inoculation. Epifluorescence microscopy analyses revealed a high callose deposition in the sieve tube elements of leaf tissues in some transgenic events. Callose accumulation is an important plant defense response to biotic stresses and, in csd1 transgenic lines, it was associated with lower Xcc populations.
650 $aAntimicrobial peptides
650 $aCitrus
650 $aSuperoxide dismutase
650 $aTransgenic plants
650 $aXanthomonas citri subsp. citri
650 $aFruta Cítrica
650 $aPlanta Transgênica
700 1 $aMORAES, T. DE S.
700 1 $aATTÍLIO, L. B.
700 1 $aMARQUES, J. P. R.
700 1 $aSTIPP, L. C. L.
700 1 $aHARAKAVA, R.
700 1 $aASTUA, J. de F.
700 1 $aBELASQUE JUNIOR, J.
700 1 $aAZEVEDO, R. A.
700 1 $aGAZIOLA, S. A.
700 1 $aMOURÃO FILHO, F. DE A. A.
773 $tPlant Cell, Tissue and Organ Culture, 2022.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Gado de Corte. |
|
Data corrente: |
18/02/2013 |
|
Data da última atualização: |
18/02/2013 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
OLIVEIRA, C. C. de; VILLELA, S. D. J.; ALMEIDA, R. G. de; ALVES, F. V.; BUNGENSTAB, D. J. |
|
Afiliação: |
Caroline Carvalho de Oliveira, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM); Severino Delmar Junqueira Villela; ROBERTO GIOLO DE ALMEIDA, CNPGC; FABIANA VILLA ALVES, CNPGC; DAVI JOSE BUNGENSTAB, CNPGC. |
|
Título: |
Black globe humidity index (BGHI) in integrated crop-livestock-forest systems. |
|
Ano de publicação: |
2012 |
|
Fonte/Imprenta: |
IN: INTERNATIONAL CONGRESS SAVANNAS AND WETLANDS. Rural sustainable development. Aquidauana: UEMS, 2012. |
|
Páginas: |
1 p. |
|
Idioma: |
Português |
|
Notas: |
RSD |
|
Palavras-Chave: |
Integração lavoura-pecuária-floresta. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 00623nam a2200181 a 4500
001 1949804
005 2013-02-18
008 2012 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, C. C. de
245 $aBlack globe humidity index (BGHI) in integrated crop-livestock-forest systems.$h[electronic resource]
260 $aIN: INTERNATIONAL CONGRESS SAVANNAS AND WETLANDS. Rural sustainable development. Aquidauana: UEMS$c2012
300 $a1 p.
500 $aRSD
653 $aIntegração lavoura-pecuária-floresta
700 1 $aVILLELA, S. D. J.
700 1 $aALMEIDA, R. G. de
700 1 $aALVES, F. V.
700 1 $aBUNGENSTAB, D. J.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Corte (CNPGC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
