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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
24/02/2023 |
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Data da última atualização: |
08/03/2023 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
MIZUBUTI, E. S. G.; YAMADA, J. K.; SANTIAGO, T. R.; LOPES, C. A. |
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Afiliação: |
EDUARDO S. G. MIZUBUTI, UNIVERSIDADE FEDERAL DE VIÇOSA; JAQUELINE K. YAMADA, UNIVERSIDADE FEDERAL DE VIÇOSA; THAÍS R. SANTIAGO, UNIVERSIDADE DE BRASÍLIA; CARLOS ALBERTO LOPES, CNPH. |
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Título: |
On the assessment of the sources of inoculum of bacterial wilt in Brazil. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Tropical Plant Pathology, v. 47, p. 685-692, 2022. |
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ISSN: |
1983-2052 |
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DOI: |
https://doi.org/10.1101/2021.12.23.473731 |
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Idioma: |
Inglês |
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Conteúdo: |
Dispersal of Ralstonia spp. cells by water and contaminated plant material and the importance of weeds as inoculum sources have been poorly investigated. Water of rivers, soil from fields of diverse crops and areas of natural vegetation both from the Amazônia, Cerrado and Mata Atlântica biomes, besides soil of the rhizosphere of weeds present in tomato fields with records of bacterial wilt were sampled and analyzed to detect Ralstonia spp. Seeds of tomato plants artificially and naturally infected with Ralstonia spp. were also processed. All samples were enriched a priori in selective medium South Africa (SMSA) and colonies were isolated in plates containing solid SMSA. Detection of Ralstonia spp. was confirmed by polymerase chain reaction with specific primers. The Co ? operational PCR (CO-PCR) was also used to detect Ralstonia spp. Colonies were obtained from soil samples and from a commercial substrate sample. Five soil samples from eggplant fields, one from coffee field, one substrate from potato seed tuber production, two soil samples from the rhizosphere of Amaranthus spp., one from Bidens pilosa and one from Solanum americanum tested positive for Ralstonia spp. Besides these soil samples, five water samples of rivers were positive for CO-PCR detection: two samples from Amazônia, one from Cerrado and two samples from irrigation water collected from tomato fields located in the Mata Atlântica biome. Ralstonia spp. were not detected in tomato seeds. These results revealed potential inoculum sources, especially weeds, in areas with historical records of bacterial wilt. Additionally, rivers may act as dispersal agents of inoculum of Ralstonia spp. MenosDispersal of Ralstonia spp. cells by water and contaminated plant material and the importance of weeds as inoculum sources have been poorly investigated. Water of rivers, soil from fields of diverse crops and areas of natural vegetation both from the Amazônia, Cerrado and Mata Atlântica biomes, besides soil of the rhizosphere of weeds present in tomato fields with records of bacterial wilt were sampled and analyzed to detect Ralstonia spp. Seeds of tomato plants artificially and naturally infected with Ralstonia spp. were also processed. All samples were enriched a priori in selective medium South Africa (SMSA) and colonies were isolated in plates containing solid SMSA. Detection of Ralstonia spp. was confirmed by polymerase chain reaction with specific primers. The Co ? operational PCR (CO-PCR) was also used to detect Ralstonia spp. Colonies were obtained from soil samples and from a commercial substrate sample. Five soil samples from eggplant fields, one from coffee field, one substrate from potato seed tuber production, two soil samples from the rhizosphere of Amaranthus spp., one from Bidens pilosa and one from Solanum americanum tested positive for Ralstonia spp. Besides these soil samples, five water samples of rivers were positive for CO-PCR detection: two samples from Amazônia, one from Cerrado and two samples from irrigation water collected from tomato fields located in the Mata Atlântica biome. Ralstonia spp. were not detected in tomato seeds. These results reveale... Mostrar Tudo |
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Thesagro: |
Batata; Berinjela; Café; Murcha Bacteriana; Ralstonia Solanacearum; Solo; Tomate. |
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Thesaurus Nal: |
Bacterial wilt. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/247541/1/Tropical-plant-Pathology-v.-47-p-685-692-2022.pdf
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Marc: |
LEADER 02455naa a2200277 a 4500
001 2151941
005 2023-03-08
008 2022 bl uuuu u00u1 u #d
022 $a1983-2052
024 7 $ahttps://doi.org/10.1101/2021.12.23.473731$2DOI
100 1 $aMIZUBUTI, E. S. G.
245 $aOn the assessment of the sources of inoculum of bacterial wilt in Brazil.$h[electronic resource]
260 $c2022
520 $aDispersal of Ralstonia spp. cells by water and contaminated plant material and the importance of weeds as inoculum sources have been poorly investigated. Water of rivers, soil from fields of diverse crops and areas of natural vegetation both from the Amazônia, Cerrado and Mata Atlântica biomes, besides soil of the rhizosphere of weeds present in tomato fields with records of bacterial wilt were sampled and analyzed to detect Ralstonia spp. Seeds of tomato plants artificially and naturally infected with Ralstonia spp. were also processed. All samples were enriched a priori in selective medium South Africa (SMSA) and colonies were isolated in plates containing solid SMSA. Detection of Ralstonia spp. was confirmed by polymerase chain reaction with specific primers. The Co ? operational PCR (CO-PCR) was also used to detect Ralstonia spp. Colonies were obtained from soil samples and from a commercial substrate sample. Five soil samples from eggplant fields, one from coffee field, one substrate from potato seed tuber production, two soil samples from the rhizosphere of Amaranthus spp., one from Bidens pilosa and one from Solanum americanum tested positive for Ralstonia spp. Besides these soil samples, five water samples of rivers were positive for CO-PCR detection: two samples from Amazônia, one from Cerrado and two samples from irrigation water collected from tomato fields located in the Mata Atlântica biome. Ralstonia spp. were not detected in tomato seeds. These results revealed potential inoculum sources, especially weeds, in areas with historical records of bacterial wilt. Additionally, rivers may act as dispersal agents of inoculum of Ralstonia spp.
650 $aBacterial wilt
650 $aBatata
650 $aBerinjela
650 $aCafé
650 $aMurcha Bacteriana
650 $aRalstonia Solanacearum
650 $aSolo
650 $aTomate
700 1 $aYAMADA, J. K.
700 1 $aSANTIAGO, T. R.
700 1 $aLOPES, C. A.
773 $tTropical Plant Pathology$gv. 47, p. 685-692, 2022.
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Embrapa Hortaliças (CNPH) |
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