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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
14/02/2023 |
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Data da última atualização: |
26/04/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROGÉRIO, F.; BARONCELLI, R.; CUEVAS-FERNÁNDEZ, F. B.; BECERRA, S.; CROUCH, J.; BETTIOL, W.; AZCÁRATE-PERIL, M. A.; MALAPI-WIGHT, M.; ORTEGA, V.; BETRAN, J.; TENUTA, A.; DAMBOLENA, J. S.; ESKER, P. D.; REVILLA, P.; JACKSON-ZIEMS; HILTBRUNNER, J.; MUNKVOLD, G.; BUHINICEK, I.; VICENTE-VILLARDÓN, J. L.''''; SUKNO, S. A.; THON, M. R. |
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Afiliação: |
FLÁVIA ROGÉRIO, UNIVERSIDAD DE SALAMANCA; RICCARDO BARONCELLI, UNIVERSITÀ DI BOLOGNA; FRANCISCO BORJA CUEVAS-FERNÁNDEZ, UNIVERSIDAD DE SALAMANCA; SIOLY BECERRA, UNIVERSIDAD DE SALAMANCA; JOANNE CROUCH, UNITED STATES DEPARTMENT OF AGRICULTURE; WAGNER BETTIOL, CNPMA; MARIA ANDREA AZCÁRATE-PERIL, UNIVERSITY OF NORTH CAROLINE; MARTHA MALAPI-WIGHT, UNITED STATES DEPARTMENT OF AGRICULTURE; VERONIQUE ORTEGA, SYNGENTA SEEDS; JAVIER BETRAN, BAYER CROP SCIENCE; ALBERT TENUTA, UNIVERSITY OF GUELPH-RIDGETOWN; JOSÉ S DAMBOLENA, UNIVERSIDAD NACIONAL DE CÓRDOBA; PAUL D ESKER, THE PENNSYLVANIA STATE UNIVERSITY; PEDRO REVILLA, CONSEJO SUPERIOR DE INVESTIGACIONES CIENTÍFICAS; TAMRA A JACKSON-ZIEMS, UNIVERSITY OF NEBRASKA-LINCOLN; JÜRG HILTBRUNNER, DÉPARTEMENT FÉDÉRAL DE L'ÉCONOMIE, DE LA FORMATION ET DE LA RECHERCHE; GARY MUNKVOLD, IOWA STATE UNIVERSITY; IVICA BUHINICEK, BC INSTITUTE FOR BREEDING AND PRODUCTION OF FIELD CROPS; JOSÉ LUIS VICENTE-VILLARDÓN, UNIVERSIDAD DE SALAMANCA; SERENELLA A SUKNO, UNIVERSIDAD DE SALAMANCA; MICHAEL R THON, UNIVERSIDAD DE SALAMANCA. |
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Título: |
Population genomics provide insights into the global genetic structure of Colletotrichum graminicola, the causal agent of maize anthracnose. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
MBio, v. 14, n. 1, e0287822, 2022. |
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ISSN: |
2150-7511 |
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DOI: |
https://doi.org/10.1128/mbio.02878-22 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Understanding the genetic diversity and mechanisms underlying genetic variation in pathogen populations is crucial to the development of effective control strategies. We investigated the genetic diversity and reproductive biology of Colletotrichum graminicola isolates which infect maize by sequencing the genomes of 108 isolates collected from 14 countries using restriction site-associated DNA sequencing (RAD-seq) and whole-genome sequencing (WGS). Clustering analyses based on single-nucleotide polymorphisms revealed three genetic groups delimited by continental origin, compatible with short-dispersal of the pathogen and geographic subdivision. Intra- and intercontinental migration was observed between Europe and South America, likely associated with the movement of contaminated germplasm. Low clonality, evidence of genetic recombination, and high phenotypic diversity were detected. We show evidence that, although it is rare (possibly due to losses of sexual reproduction- and meiosis-associated genes) C. graminicola can undergo sexual recombination. Our results support the hypotheses that intra- and intercontinental pathogen migration and genetic recombination have great impacts on the C. graminicola population structure. Importance: Plant pathogens cause significant reductions in yield and crop quality and cause enormous economic losses worldwide. Reducing these losses provides an obvious strategy to increase food production without further degrading natural ecosystems; however, this requires knowledge of the biology and evolution of the pathogens in agroecosystems. We employed a population genomics approach to investigate the genetic diversity and reproductive biology of the maize anthracnose pathogen (Colletotrichum graminicola) in 14 countries. We found that the populations are correlated with their geographical origin and that migration between countries is ongoing, possibly caused by the movement of infected plant material. This result has direct implications for disease management because migration can cause the movement of more virulent and/or fungicide-resistant genotypes. We conclude that genetic recombination is frequent (in contrast to the traditional view of C. graminicola being mainly asexual), which strongly impacts control measures and breeding programs aimed at controlling this disease. MenosAbstract: Understanding the genetic diversity and mechanisms underlying genetic variation in pathogen populations is crucial to the development of effective control strategies. We investigated the genetic diversity and reproductive biology of Colletotrichum graminicola isolates which infect maize by sequencing the genomes of 108 isolates collected from 14 countries using restriction site-associated DNA sequencing (RAD-seq) and whole-genome sequencing (WGS). Clustering analyses based on single-nucleotide polymorphisms revealed three genetic groups delimited by continental origin, compatible with short-dispersal of the pathogen and geographic subdivision. Intra- and intercontinental migration was observed between Europe and South America, likely associated with the movement of contaminated germplasm. Low clonality, evidence of genetic recombination, and high phenotypic diversity were detected. We show evidence that, although it is rare (possibly due to losses of sexual reproduction- and meiosis-associated genes) C. graminicola can undergo sexual recombination. Our results support the hypotheses that intra- and intercontinental pathogen migration and genetic recombination have great impacts on the C. graminicola population structure. Importance: Plant pathogens cause significant reductions in yield and crop quality and cause enormous economic losses worldwide. Reducing these losses provides an obvious strategy to increase food production without further degrading natural ecosys... Mostrar Tudo |
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Thesagro: |
Antracnose; Colletotrichum Graminicola; Milho; Variação Genética. |
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Thesaurus Nal: |
Anthracnose; Corn; Fungal diseases of plants; Genetic recombination; Genomics; Phylogeography; Population genetics. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/249422/1/Bettiol-Population-Genomics-2023.pdf
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Marc: |
LEADER 03830naa a2200517 a 4500
001 2151733
005 2023-04-26
008 2022 bl uuuu u00u1 u #d
022 $a2150-7511
024 7 $ahttps://doi.org/10.1128/mbio.02878-22$2DOI
100 1 $aROGÉRIO, F.
245 $aPopulation genomics provide insights into the global genetic structure of Colletotrichum graminicola, the causal agent of maize anthracnose.$h[electronic resource]
260 $c2022
520 $aAbstract: Understanding the genetic diversity and mechanisms underlying genetic variation in pathogen populations is crucial to the development of effective control strategies. We investigated the genetic diversity and reproductive biology of Colletotrichum graminicola isolates which infect maize by sequencing the genomes of 108 isolates collected from 14 countries using restriction site-associated DNA sequencing (RAD-seq) and whole-genome sequencing (WGS). Clustering analyses based on single-nucleotide polymorphisms revealed three genetic groups delimited by continental origin, compatible with short-dispersal of the pathogen and geographic subdivision. Intra- and intercontinental migration was observed between Europe and South America, likely associated with the movement of contaminated germplasm. Low clonality, evidence of genetic recombination, and high phenotypic diversity were detected. We show evidence that, although it is rare (possibly due to losses of sexual reproduction- and meiosis-associated genes) C. graminicola can undergo sexual recombination. Our results support the hypotheses that intra- and intercontinental pathogen migration and genetic recombination have great impacts on the C. graminicola population structure. Importance: Plant pathogens cause significant reductions in yield and crop quality and cause enormous economic losses worldwide. Reducing these losses provides an obvious strategy to increase food production without further degrading natural ecosystems; however, this requires knowledge of the biology and evolution of the pathogens in agroecosystems. We employed a population genomics approach to investigate the genetic diversity and reproductive biology of the maize anthracnose pathogen (Colletotrichum graminicola) in 14 countries. We found that the populations are correlated with their geographical origin and that migration between countries is ongoing, possibly caused by the movement of infected plant material. This result has direct implications for disease management because migration can cause the movement of more virulent and/or fungicide-resistant genotypes. We conclude that genetic recombination is frequent (in contrast to the traditional view of C. graminicola being mainly asexual), which strongly impacts control measures and breeding programs aimed at controlling this disease.
650 $aAnthracnose
650 $aCorn
650 $aFungal diseases of plants
650 $aGenetic recombination
650 $aGenomics
650 $aPhylogeography
650 $aPopulation genetics
650 $aAntracnose
650 $aColletotrichum Graminicola
650 $aMilho
650 $aVariação Genética
700 1 $aBARONCELLI, R.
700 1 $aCUEVAS-FERNÁNDEZ, F. B.
700 1 $aBECERRA, S.
700 1 $aCROUCH, J.
700 1 $aBETTIOL, W.
700 1 $aAZCÁRATE-PERIL, M. A.
700 1 $aMALAPI-WIGHT, M.
700 1 $aORTEGA, V.
700 1 $aBETRAN, J.
700 1 $aTENUTA, A.
700 1 $aDAMBOLENA, J. S.
700 1 $aESKER, P. D.
700 1 $aREVILLA, P.
700 1 $aJACKSON-ZIEMS
700 1 $aHILTBRUNNER, J.
700 1 $aMUNKVOLD, G.
700 1 $aBUHINICEK, I.
700 1 $aVICENTE-VILLARDÓN, J. L.''''
700 1 $aSUKNO, S. A.
700 1 $aTHON, M. R.
773 $tMBio$gv. 14, n. 1, e0287822, 2022.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/02/2023 |
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Data da última atualização: |
01/02/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
RODRIGUES, J. N. D.; GUIMARÃES, J. D.; RANGEL, P. S. C.; OLIVEIRA, M. E. F.; BRANDÃO, F. Z.; BARTLEWSKI, P. M.; FONSECA, J. F. da. |
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Afiliação: |
JULIANA NASCIMENTO DUARTE RODRIGUES; JOSÉ DOMINGOS GUIMARÃES; PAULO SÉRGIO CERQUEIRA RANGEL; MARIA EMILIA FRANCO OLIVEIRA; FELIPE ZANDONADI BRANDÃO; PAWEL MIECZYSLAW BARTLEWSKI; JEFERSON FERREIRA DA FONSECA, CNPC. |
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Título: |
Luteal function in cyclic goats treated with human chorionic gonadotropin administered by intramuscular or intravaginal routes at the time of artificial insemination. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Reproduction in Domestic Animal, p. 1-9, Nov. 2022. |
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DOI: |
https://doi.org/10.1111/rda.14298 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Human chorionic gonadotropin (hCG) has been used to improve goats reproductive efficiency. This study aimed to (i) evaluate if hCG administered by the intramuscular (i.m.) or intravaginal (i.vag.) route can be detected by a rapid ?-hCG test in blood plasma samples and (ii) document ovarian effects of hCG administered by both routes at the time of artificial insemination (AI) performed 60?h after oestrus synchronization in goats. Twenty-two Alpine goats received two i.m. injections of 30??g of d-cloprostenol (Prolise®, Tecnopec, São Paulo, Brazil) 7.5?days apart. One day after the onset of oestrus (at the time of AI), the goats were randomly allocated to one of the three groups that received: control (n = 7): 0.3 ml of saline solution intravaginally; hCGi.m. (n = 7): 300?IU of hCG (Vetecor®; Hertape-Calier, São Paulo, Brazil) i.m. and hCGi.vag. (n = 8): 300?IU of hCG deposited intravaginally. Blood samples were drawn at ?1, 3, 6, 9 and 24?h after as well as on days 3, 7, 10, 13, 17 and 21 after hCG treatment/AI. All animals tested negative for hCG (ECO Diagnóstica, Corinto, Brazil) at ?1 h, and all control animals tested negative throughout the entire blood collection period. All hCGi.m. animals tested positive from 3 h until D3 post-AI but only 50% of hCGi.vag. goats tested positive during the present study. In all animals studied, mean circulating P4 concentrations increased (p?MenosAbstract: Human chorionic gonadotropin (hCG) has been used to improve goats reproductive efficiency. This study aimed to (i) evaluate if hCG administered by the intramuscular (i.m.) or intravaginal (i.vag.) route can be detected by a rapid ?-hCG test in blood plasma samples and (ii) document ovarian effects of hCG administered by both routes at the time of artificial insemination (AI) performed 60?h after oestrus synchronization in goats. Twenty-two Alpine goats received two i.m. injections of 30??g of d-cloprostenol (Prolise®, Tecnopec, São Paulo, Brazil) 7.5?days apart. One day after the onset of oestrus (at the time of AI), the goats were randomly allocated to one of the three groups that received: control (n = 7): 0.3 ml of saline solution intravaginally; hCGi.m. (n = 7): 300?IU of hCG (Vetecor®; Hertape-Calier, São Paulo, Brazil) i.m. and hCGi.vag. (n = 8): 300?IU of hCG deposited intravaginally. Blood samples were drawn at ?1, 3, 6, 9 and 24?h after as well as on days 3, 7, 10, 13, 17 and 21 after hCG treatment/AI. All animals tested negative for hCG (ECO Diagnóstica, Corinto, Brazil) at ?1 h, and all control animals tested negative throughout the entire blood collection period. All hCGi.m. animals tested positive from 3 h until D3 post-AI but only 50% of hCGi.vag. goats tested positive during the present study. In all animals studied, mean circulating P4 concentrations increased (p?Mostrar Tudo |
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Palavras-Chave: |
HCG; Luteal function; Pregnancy rates. |
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Thesaurus NAL: |
Corpus luteum; Goats; Human chorionic gonadotropin; Reproduction; Ultrasonography. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03055naa a2200301 a 4500
001 2151432
005 2023-02-01
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1111/rda.14298$2DOI
100 1 $aRODRIGUES, J. N. D.
245 $aLuteal function in cyclic goats treated with human chorionic gonadotropin administered by intramuscular or intravaginal routes at the time of artificial insemination.$h[electronic resource]
260 $c2022
520 $aAbstract: Human chorionic gonadotropin (hCG) has been used to improve goats reproductive efficiency. This study aimed to (i) evaluate if hCG administered by the intramuscular (i.m.) or intravaginal (i.vag.) route can be detected by a rapid ?-hCG test in blood plasma samples and (ii) document ovarian effects of hCG administered by both routes at the time of artificial insemination (AI) performed 60?h after oestrus synchronization in goats. Twenty-two Alpine goats received two i.m. injections of 30??g of d-cloprostenol (Prolise®, Tecnopec, São Paulo, Brazil) 7.5?days apart. One day after the onset of oestrus (at the time of AI), the goats were randomly allocated to one of the three groups that received: control (n = 7): 0.3 ml of saline solution intravaginally; hCGi.m. (n = 7): 300?IU of hCG (Vetecor®; Hertape-Calier, São Paulo, Brazil) i.m. and hCGi.vag. (n = 8): 300?IU of hCG deposited intravaginally. Blood samples were drawn at ?1, 3, 6, 9 and 24?h after as well as on days 3, 7, 10, 13, 17 and 21 after hCG treatment/AI. All animals tested negative for hCG (ECO Diagnóstica, Corinto, Brazil) at ?1 h, and all control animals tested negative throughout the entire blood collection period. All hCGi.m. animals tested positive from 3 h until D3 post-AI but only 50% of hCGi.vag. goats tested positive during the present study. In all animals studied, mean circulating P4 concentrations increased (p?<?.05) from D3 to D7 after AI and then declined (p?<?.05) from D10 to D17 in control and hCGi.m. groups and from D17 to D21 in the hCGi.vag. group. Total cross-sectional luteal area (CLA), mean colour Doppler area (DA), DA/CLA, mean high-velocity Doppler area and HVDA/CLA all declined (p?<?.05) by D17?D21 in all animals studied. In summary: (i) human chorionic gonadotropin could consistently be detected in blood samples using the rapid ?-hCG test only in the hCGi.m. group; and (ii) there were no significant differences in the mean pregnancy rate, circulating P4 concentrations and various luteal parameters studied among Control, hCGi.m. and hCGi.vag. dose.
650 $aCorpus luteum
650 $aGoats
650 $aHuman chorionic gonadotropin
650 $aReproduction
650 $aUltrasonography
653 $aHCG
653 $aLuteal function
653 $aPregnancy rates
700 1 $aGUIMARÃES, J. D.
700 1 $aRANGEL, P. S. C.
700 1 $aOLIVEIRA, M. E. F.
700 1 $aBRANDÃO, F. Z.
700 1 $aBARTLEWSKI, P. M.
700 1 $aFONSECA, J. F. da
773 $tReproduction in Domestic Animal, p. 1-9, Nov. 2022.
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