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Registro Completo |
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Biblioteca(s): |
Embrapa Algodão; Embrapa Territorial. |
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Data corrente: |
20/07/2022 |
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Data da última atualização: |
04/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
HOFFMANN, L. V.; BRANQUINHO, A. A.; BARROSO, P. A. V.; VASLIN, M. F. S. |
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Afiliação: |
LUCIA VIEIRA HOFFMANN, CNPA; AMANDA ALVES BRANQUINHO, UNIVERSIDADE FEDERAL DE GOIÁS; PAULO AUGUSTO VIANNA BARROSO, CNPM; MAITE F. S. VASLIN, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO. |
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Título: |
Antibodies for the coat protein of cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Frontiers in Plant Science, v. 13, article 814119, 5 p. 2022. |
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ISBN: |
1664-462X |
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DOI: |
10.3389/fpls.2022.814119 |
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Idioma: |
Inglês |
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Conteúdo: |
The cotton blue disease, caused by the cotton leafroll dwarf virus (CLRDV), leads to dwarfism, leaf rolling, and production loss in susceptible cotton varieties. To develop an enzyme-linked immunosorbent assay (ELISA) test to detect the virus in cotton and weeds, peptides based on the coat protein were used to produce polyclonal (a-GQE, a-PRN, and a-INK) and monoclonal (a-GQE, a-PRN, and a-NKF) antibodies. All six were tested as capture antibodies, and polyclonal a-GQE and the monocle onal a-NKF were labeled with the enzyme alkaline phosphatase and used as detection antibodies for a double antibody sandwich (DAS) ELISA method, in which p-nitrophenyl phosphate was added and measured by absorbance at 405 nm. The DAS-ELISA sandwich was efficient in discriminating between healthy and diseased plant extracts. The ELISA methodology detected the virus in the weeds Commelina sp., which was confirmed by RT-PCR. The monoclonal antibodies may be used to develop other diagnostic procedures. |
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Palavras-Chave: |
CLRDV; Enzyme-linked immunosorbent assay (ELISA); Intermediary host. |
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Thesagro: |
Vírus. |
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Thesaurus Nal: |
Serology. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1144832/1/6046.pdf
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Marc: |
LEADER 01770naa a2200241 a 4500
001 2151489
005 2023-05-04
008 2022 bl uuuu u00u1 u #d
022 $a1664-462X
024 7 $a10.3389/fpls.2022.814119$2DOI
100 1 $aHOFFMANN, L. V.
245 $aAntibodies for the coat protein of cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease.$h[electronic resource]
260 $c2022
520 $aThe cotton blue disease, caused by the cotton leafroll dwarf virus (CLRDV), leads to dwarfism, leaf rolling, and production loss in susceptible cotton varieties. To develop an enzyme-linked immunosorbent assay (ELISA) test to detect the virus in cotton and weeds, peptides based on the coat protein were used to produce polyclonal (a-GQE, a-PRN, and a-INK) and monoclonal (a-GQE, a-PRN, and a-NKF) antibodies. All six were tested as capture antibodies, and polyclonal a-GQE and the monocle onal a-NKF were labeled with the enzyme alkaline phosphatase and used as detection antibodies for a double antibody sandwich (DAS) ELISA method, in which p-nitrophenyl phosphate was added and measured by absorbance at 405 nm. The DAS-ELISA sandwich was efficient in discriminating between healthy and diseased plant extracts. The ELISA methodology detected the virus in the weeds Commelina sp., which was confirmed by RT-PCR. The monoclonal antibodies may be used to develop other diagnostic procedures.
650 $aSerology
650 $aVírus
653 $aCLRDV
653 $aEnzyme-linked immunosorbent assay (ELISA)
653 $aIntermediary host
700 1 $aBRANQUINHO, A. A.
700 1 $aBARROSO, P. A. V.
700 1 $aVASLIN, M. F. S.
773 $tFrontiers in Plant Science$gv. 13, article 814119, 5 p. 2022.
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Embrapa Algodão (CNPA) |
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