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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
29/12/2022 |
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Data da última atualização: |
13/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
KATAYAMA, E.; RODRIGUES, N. A.; BILATTO, S.; CASCIATORI, F. P.; FARINAS, C. S. |
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Afiliação: |
CRISTIANE SANCHEZ FARINAS, CNPDIA. |
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Título: |
Nanocellulose isolation using a thermostable endoglucanase-rich cocktail from Myceliophthora thermophila cultivated in a multilayer packed-bed bioreactor. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Biomass Conversion and Biorefnery, 2022. |
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DOI: |
https://doi.org/10.1007/s13399-022-02977-1 |
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Idioma: |
Inglês |
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Notas: |
First on line. |
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Conteúdo: |
The isolation of nanocellulose materials by means of enzymatic hydrolysis ofers advantages in terms of their properties and operational conditions, but there is still a lack of enzymatic cocktails specifcally designed for this application. The present work investigates the use of a thermostable endoglucanase-rich enzymatic cocktail from Myceliophthora thermophila for nanocellulose production. An initial set of experiments was conducted to determine the optimal conditions for enzyme production under solid-state cultivation in small-scale polypropylene plastic bags. A full factorial experimental design was used as a statistical tool to evaluate the efects of the solid substrate and moisture content on the production of endoglucanase and ?-glucosidase. Solid-state cultivations in a multilayer packed-bed bioreactor were then carried out under the selected conditions to obtain a larger volume of an enzymatic extract with high endoglucanase selectivity, given by the ratio of endoglucanase (203±6 U/g-substrate) to ?-glucosidase activity (1.6±0.1 U/g-substrate). The enzymatic hydrolysis of eucalyptus cellulose pulp at diferent temperatures, with this endoglucanase-rich cocktail at a loading of 10 mg-protein/g-pulp, resulted in higher glucose release (11±2 g-glucose/L) at 60 °C, with 61±9% cellulose conversion and 16.2% nanocellulose yield. The isolated nanomaterial with average diameter of 36.65 nm and length of 156.63 nm, presented a crystallinity index of 77.4% and thermal stability temperature of 316 °C. These results show the potential to isolate nanocellulose using a thermostable endoglucanase-rich enzymatic cocktail produced locally by solid-state cultivation in a packed-bed bioreactor, in accordance to the biorefnery concept. MenosThe isolation of nanocellulose materials by means of enzymatic hydrolysis ofers advantages in terms of their properties and operational conditions, but there is still a lack of enzymatic cocktails specifcally designed for this application. The present work investigates the use of a thermostable endoglucanase-rich enzymatic cocktail from Myceliophthora thermophila for nanocellulose production. An initial set of experiments was conducted to determine the optimal conditions for enzyme production under solid-state cultivation in small-scale polypropylene plastic bags. A full factorial experimental design was used as a statistical tool to evaluate the efects of the solid substrate and moisture content on the production of endoglucanase and ?-glucosidase. Solid-state cultivations in a multilayer packed-bed bioreactor were then carried out under the selected conditions to obtain a larger volume of an enzymatic extract with high endoglucanase selectivity, given by the ratio of endoglucanase (203±6 U/g-substrate) to ?-glucosidase activity (1.6±0.1 U/g-substrate). The enzymatic hydrolysis of eucalyptus cellulose pulp at diferent temperatures, with this endoglucanase-rich cocktail at a loading of 10 mg-protein/g-pulp, resulted in higher glucose release (11±2 g-glucose/L) at 60 °C, with 61±9% cellulose conversion and 16.2% nanocellulose yield. The isolated nanomaterial with average diameter of 36.65 nm and length of 156.63 nm, presented a crystallinity index of 77.4% and thermal stabili... Mostrar Tudo |
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Palavras-Chave: |
Endoglucanase; Packed-bed bioreactor; Solid-state cultivation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02529naa a2200229 a 4500
001 2150493
005 2023-01-13
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s13399-022-02977-1$2DOI
100 1 $aKATAYAMA, E.
245 $aNanocellulose isolation using a thermostable endoglucanase-rich cocktail from Myceliophthora thermophila cultivated in a multilayer packed-bed bioreactor.$h[electronic resource]
260 $c2022
500 $aFirst on line.
520 $aThe isolation of nanocellulose materials by means of enzymatic hydrolysis ofers advantages in terms of their properties and operational conditions, but there is still a lack of enzymatic cocktails specifcally designed for this application. The present work investigates the use of a thermostable endoglucanase-rich enzymatic cocktail from Myceliophthora thermophila for nanocellulose production. An initial set of experiments was conducted to determine the optimal conditions for enzyme production under solid-state cultivation in small-scale polypropylene plastic bags. A full factorial experimental design was used as a statistical tool to evaluate the efects of the solid substrate and moisture content on the production of endoglucanase and ?-glucosidase. Solid-state cultivations in a multilayer packed-bed bioreactor were then carried out under the selected conditions to obtain a larger volume of an enzymatic extract with high endoglucanase selectivity, given by the ratio of endoglucanase (203±6 U/g-substrate) to ?-glucosidase activity (1.6±0.1 U/g-substrate). The enzymatic hydrolysis of eucalyptus cellulose pulp at diferent temperatures, with this endoglucanase-rich cocktail at a loading of 10 mg-protein/g-pulp, resulted in higher glucose release (11±2 g-glucose/L) at 60 °C, with 61±9% cellulose conversion and 16.2% nanocellulose yield. The isolated nanomaterial with average diameter of 36.65 nm and length of 156.63 nm, presented a crystallinity index of 77.4% and thermal stability temperature of 316 °C. These results show the potential to isolate nanocellulose using a thermostable endoglucanase-rich enzymatic cocktail produced locally by solid-state cultivation in a packed-bed bioreactor, in accordance to the biorefnery concept.
653 $aEndoglucanase
653 $aPacked-bed bioreactor
653 $aSolid-state cultivation
700 1 $aRODRIGUES, N. A.
700 1 $aBILATTO, S.
700 1 $aCASCIATORI, F. P.
700 1 $aFARINAS, C. S.
773 $tBiomass Conversion and Biorefnery, 2022.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
01/12/2011 |
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Data da última atualização: |
01/12/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
TAVARES, P.; BERGMANN, J.; KRUGER, R. H.; NORONHA, E.; QUIRINO, B. F. |
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Afiliação: |
P. Tavares, UNB; J. Bergmann, UCB; R. H. Kruger, UNB; E. Noronha, UNB; BETANIA FERRAZ QUIRINO, CNPAE. |
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Título: |
Accessing lipases from soil metagenome as an aid for biofuel production. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: BRAZILIAN BIOENERGY SCIENCE AND TECHNOLOGY CONFERENCE - BBEST, 1., 2011, Campos do Jordão, SP. [Anais...]. São Paulo: FAPESP, 2011. |
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Páginas: |
Não paginado. |
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Idioma: |
Inglês |
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Palavras-Chave: |
Biofuel production; Lipases; Soil metagenome. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/48656/1/Betania-BBEST-2011-4.pdf
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Marc: |
LEADER 00655nam a2200193 a 4500
001 1908059
005 2011-12-01
008 2011 bl uuuu u00u1 u #d
100 1 $aTAVARES, P.
245 $aAccessing lipases from soil metagenome as an aid for biofuel production.$h[electronic resource]
260 $aIn: BRAZILIAN BIOENERGY SCIENCE AND TECHNOLOGY CONFERENCE - BBEST, 1., 2011, Campos do Jordão, SP. [Anais...]. São Paulo: FAPESP$c2011
300 $aNão paginado.
653 $aBiofuel production
653 $aLipases
653 $aSoil metagenome
700 1 $aBERGMANN, J.
700 1 $aKRUGER, R. H.
700 1 $aNORONHA, E.
700 1 $aQUIRINO, B. F.
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