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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
21/12/2022 |
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Data da última atualização: |
21/12/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, A. N. da; ALVES, L.; OSOWSKI, G. V.; SABEI, L.; FERRAZ, P. A.; PUGLIESI, G.; MARQUES, M. G.; ZANELLA, R.; ZANELLA, A. J. |
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Afiliação: |
ARTHUR NERY DA SILVA, Universidade de São Paulo; LUANA ALVES, Universidade de São Paulo; GERMANA VIZZOTTO OSOWSKI, Universidade de São Paulo; LEANDRO SABEI, Universidade de São Paulo; PRISCILA ASSIS FERRAZ, Universidade de São Paulo; GUILHERME PUGLIESI, Universidade de São Paulo; MARIANA GROKE MARQUES, CNPSA; RICARDO ZANELLA, Universidade de Passo Fundo; ADROALDO JOSÉ ZANELLA, Universidade de São Paulo. |
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Título: |
Housing Conditions and a Challenge with Lipopolysaccharide on the Day of Estrus Can Influence Gene Expression of the Corpus Luteum in Gilts. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Genes, v. 13, n. 5, p. 1-13, 2022. |
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DOI: |
https://doi.org/10.3390/genes13050769 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The corpus luteum (CL) is a temporary endocrine gland that plays a decisive role in the reproductive physiology of gilts. Recently, it has been suggested that exogenous factors may compromise the normal functioning of the CL. In the present study, we aimed to understand to what extent an acute and systemic challenge with lipopolysaccharide (LPS) on the day of estrus could compromise gene expression of gilts' CLs housed in different welfare conditions. For this, we housed 42 gilts in three different housing systems: crates, indoor group pens, and outdoor housing. Then, we challenged six females from each group with LPS and eight with saline (SAL) on the day of estrus. After slaughtering the gilts on the fifth day after the challenge, ovaries were collected for gene expression analysis by RT-qPCR. Housing system and LPS challenge did not have a significant interaction for any genes evaluated; thus, their effects were studied separately. We identified significant (p < 0.05) downregulation of the angiogenic genes VEGF and FTL1 among LPS-challenged animals. Meanwhile, we also observed upregulation of HSD3B1 gene among LPS-challenged animals. We found that STAR and LHCGR genes were differentially expressed depending on the housing system, which indicates that the environment may affect adaptation capabilities. Our results indicate that an acute health challenge on the estrus day alters CL gene expression; however, the role of the housing system remains uncertain. |
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Palavras-Chave: |
Acute stress; Angiogênese; Estresse agudo; Expressão gênica; LPS; Marrãs; RT-qPCR. |
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Thesagro: |
Marra; Reprodução Animal. |
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Thesaurus Nal: |
Angiogenesis; Animal development; Gilts; Swine. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1150143/1/10086.pdf
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Marc: |
LEADER 02566naa a2200385 a 4500
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024 7 $ahttps://doi.org/10.3390/genes13050769$2DOI
100 1 $aSILVA, A. N. da
245 $aHousing Conditions and a Challenge with Lipopolysaccharide on the Day of Estrus Can Influence Gene Expression of the Corpus Luteum in Gilts.$h[electronic resource]
260 $c2022
520 $aAbstract: The corpus luteum (CL) is a temporary endocrine gland that plays a decisive role in the reproductive physiology of gilts. Recently, it has been suggested that exogenous factors may compromise the normal functioning of the CL. In the present study, we aimed to understand to what extent an acute and systemic challenge with lipopolysaccharide (LPS) on the day of estrus could compromise gene expression of gilts' CLs housed in different welfare conditions. For this, we housed 42 gilts in three different housing systems: crates, indoor group pens, and outdoor housing. Then, we challenged six females from each group with LPS and eight with saline (SAL) on the day of estrus. After slaughtering the gilts on the fifth day after the challenge, ovaries were collected for gene expression analysis by RT-qPCR. Housing system and LPS challenge did not have a significant interaction for any genes evaluated; thus, their effects were studied separately. We identified significant (p < 0.05) downregulation of the angiogenic genes VEGF and FTL1 among LPS-challenged animals. Meanwhile, we also observed upregulation of HSD3B1 gene among LPS-challenged animals. We found that STAR and LHCGR genes were differentially expressed depending on the housing system, which indicates that the environment may affect adaptation capabilities. Our results indicate that an acute health challenge on the estrus day alters CL gene expression; however, the role of the housing system remains uncertain.
650 $aAngiogenesis
650 $aAnimal development
650 $aGilts
650 $aSwine
650 $aMarra
650 $aReprodução Animal
653 $aAcute stress
653 $aAngiogênese
653 $aEstresse agudo
653 $aExpressão gênica
653 $aLPS
653 $aMarrãs
653 $aRT-qPCR
700 1 $aALVES, L.
700 1 $aOSOWSKI, G. V.
700 1 $aSABEI, L.
700 1 $aFERRAZ, P. A.
700 1 $aPUGLIESI, G.
700 1 $aMARQUES, M. G.
700 1 $aZANELLA, R.
700 1 $aZANELLA, A. J.
773 $tGenes$gv. 13, n. 5, p. 1-13, 2022.
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Embrapa Suínos e Aves (CNPSA) |
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| 1. |  | SILVA, A. N. da; ALVES, L.; OSOWSKI, G. V.; SABEI, L.; FERRAZ, P. A.; PUGLIESI, G.; MARQUES, M. G.; ZANELLA, R.; ZANELLA, A. J. Housing Conditions and a Challenge with Lipopolysaccharide on the Day of Estrus Can Influence Gene Expression of the Corpus Luteum in Gilts. Genes, v. 13, n. 5, p. 1-13, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
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