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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
05/12/2022 |
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Data da última atualização: |
13/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, E. D. de; SOUZA, J. F. da S. e; OLIVEIRA NETTO, P. M. de; CARVALHEIRA, L. de R.; BATISTA, R. I. T. P.; QUINTAO, C. C. R.; LOURO, I. D.; CAMARGO, L. S. de A. |
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Afiliação: |
ELIZA DINIZ DE SOUZA, Universidade Federal do Espírito Santo; JESSICA FERNANDA DA SILVA E SOUZA, Universidade Federal de Juiz de Fora; PEDRO MANOEL DE OLIVEIRA NETTO; LUCIANO DE REZENDE CARVALHEIRA, Universidade Federal de Minas Gerais; RIBRIO IVAN TAVARES PEREIRA BATISTA, Universidade Federal Fluminense; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; IURI DRUMOND LOURO, Universidade Federal do Espírito Santo; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Inhibition of Hsp90 during in vitro maturation under thermoneutral or heat shock conditions compromises the developmental competence of bovine oocytes. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Zygote, v. 30, n. 6, p. 854-862, 2022. |
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DOI: |
https://doi.org/10.1017/S0967199422000387 |
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Idioma: |
Inglês |
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Conteúdo: |
Heat shock protein 90 (Hsp90) is critical for cell homeostasis but its role on bovine oocyte maturation is not well known. We investigated the importance of Hsp90 for competence of bovine oocyte using 17-(allylamino)-17-demethoxygeldanamycin (17AAG), an inhibitor of Hsp90, during in vitro maturation (IVM). Three experiments evaluated the effect of 17AAG on developmental competence of oocytes matured in vitro under thermoneutral (38.5ºC) or heat shock (HS; 41.5ºC) temperatures. The first experiment found that the blastocyst rates were lower (P < 0.05) with 2 uM 17AAG compared with the untreated control (0 uM). The abundance of HSF1 transcripts was higher in oocytes matured with 2 ?M than with 0 and 1 uM 17AAG, whereas the abundance of HSP90AA1 and HSPA1A transcripts was lower (P < 0.05) with 1 and 2 uM than with 0 uM. The second experiment found that 2 ?M 17AAG for 12 or 24 h during IVM decreased (P < 0.05) the blastocysts rates. In the third experiment, the association of 2 uM 17AAG with HS for 12 h during IVM resulted in lower (P < 0.05) blastocysts rates than 17AAG, HS or untreated control. In conclusion, inhibition of Hsp90 during in vitro maturation compromises further embryo development; the association of Hsp90 inhibition with HS aggravates the deleterious effect of both on oocyte developmental competence. |
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Palavras-Chave: |
Choque térmico; Estresse por calor; Expressão genética; Geldanamicina. |
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Thesagro: |
Bovino; Ovulo. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02276naa a2200289 a 4500
001 2149206
005 2023-01-13
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/S0967199422000387$2DOI
100 1 $aSOUZA, E. D. de
245 $aInhibition of Hsp90 during in vitro maturation under thermoneutral or heat shock conditions compromises the developmental competence of bovine oocytes.$h[electronic resource]
260 $c2022
520 $aHeat shock protein 90 (Hsp90) is critical for cell homeostasis but its role on bovine oocyte maturation is not well known. We investigated the importance of Hsp90 for competence of bovine oocyte using 17-(allylamino)-17-demethoxygeldanamycin (17AAG), an inhibitor of Hsp90, during in vitro maturation (IVM). Three experiments evaluated the effect of 17AAG on developmental competence of oocytes matured in vitro under thermoneutral (38.5ºC) or heat shock (HS; 41.5ºC) temperatures. The first experiment found that the blastocyst rates were lower (P < 0.05) with 2 uM 17AAG compared with the untreated control (0 uM). The abundance of HSF1 transcripts was higher in oocytes matured with 2 ?M than with 0 and 1 uM 17AAG, whereas the abundance of HSP90AA1 and HSPA1A transcripts was lower (P < 0.05) with 1 and 2 uM than with 0 uM. The second experiment found that 2 ?M 17AAG for 12 or 24 h during IVM decreased (P < 0.05) the blastocysts rates. In the third experiment, the association of 2 uM 17AAG with HS for 12 h during IVM resulted in lower (P < 0.05) blastocysts rates than 17AAG, HS or untreated control. In conclusion, inhibition of Hsp90 during in vitro maturation compromises further embryo development; the association of Hsp90 inhibition with HS aggravates the deleterious effect of both on oocyte developmental competence.
650 $aBovino
650 $aOvulo
653 $aChoque térmico
653 $aEstresse por calor
653 $aExpressão genética
653 $aGeldanamicina
700 1 $aSOUZA, J. F. da S. e
700 1 $aOLIVEIRA NETTO, P. M. de
700 1 $aCARVALHEIRA, L. de R.
700 1 $aBATISTA, R. I. T. P.
700 1 $aQUINTAO, C. C. R.
700 1 $aLOURO, I. D.
700 1 $aCAMARGO, L. S. de A.
773 $tZygote$gv. 30, n. 6, p. 854-862, 2022.
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Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
25/03/2024 |
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Data da última atualização: |
25/03/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SOARES, F. M.; PIRES, L. F.; GARCIA, M. C.; BOUZEMBRAK, Y.; CORADIN, L.; GHILARDI-LOPES, N. P.; SILVA, R. R.; CARVALHO, A. M. de; MACULAN, B. C. M. dos S.; KOFFLER, S.; MONTEDO, U. B.; DRUCKER, D. P.; SANTIAGO, R.; GAVAI, A.; CARVALHO, M. C. P. de; LIMA, A. C. da S.; GABRIEL, H. D. E.; FRANÇA, S. G. M. de; ALMEIDA, K. R. de; SANTOS, B. J. dos; SARAIVA, A. M. |
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Afiliação: |
FILIPI MIRANDA SOARES, UNIVERSIDADE DE SÃO PAULO, UNIVERSITY OF TWENTE; LUÍS FERREIRA PIRES, UNIVERSITY OF TWENTE; MARIA CAROLINA GARCIA, CENTRO UNIVERSITÁRIO BELAS ARTES DE SÃO PAULO; YAMINE BOUZEMBRAK, WAGENINGEN UNIVERSITY AND RESEARCH; LIDIO CORADIN, PLANTS FOR THE FUTURE PROJECT; NATALIA PIRANI GHILARDI-LOPES, UNIVERSIDADE FEDERAL DO ABC; RUBENS RANGEL SILVA, CENTRO UNIVERSITÁRIO UNA; ALINE MARTINS DE CARVALHO, UNIVERSIDADE DE SÃO PAULO; BENILDES COURA MOREIRA DOS SANTOS MACULAN, UNIVERSIDADE FEDERAL DE MINAS GERAIS; SHEINA KOFFLER, UNIVERSIDADE DE SÃO PAULO; UIARA BANDINELI MONTEDO, UNIVERSIDADE DE SÃO PAULO; DEBORA PIGNATARI DRUCKER, CNPTIA; RAQUEL SANTIAGO, UNIVERSIDADE FEDERAL DE GOIÁS; ANAND GAVAI, INDUSTRIAL ENGINEERING AND BUSINESS INFORMATION SYSTEMS; MARIA CLARA PERES DE CARVALHO, UNIVERSIDADE DE SÃO PAULO; ANA CAROLINA DA SILVA LIMA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; HILLARY DANDARA ELIAS GABRIEL, UNIVERSIDADE DE SÃO PAULO; STEPHANIE GABRIELE MENDONÇA DE FRANÇA, UNIVERSIDADE DE SÃO PAULO; KAROLINE REIS DE ALMEIDA, UNIVERSIDADE DE SÃO PAULO; BÁRBARA JUNQUEIRA DOS SANTOS, UNIVERSIDADE DE SÃO PAULO; ANTONIO MAURO SARAIVA, UNIVERSIDADE DE SÃO PAULO. |
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Título: |
Leveraging citizen science for monitoring urban forageable plants. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
GigaScience, v. 13, n. 1, giae007, 2024 |
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DOI: |
10.1093/gigascience/giae007 |
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Idioma: |
Inglês |
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Conteúdo: |
Urbanization brings forth social challenges in emerging countries such as Brazil, encompassing food scarcity, health deterioration, air pollution, and biodi v ersity loss. Despite this, urban areas like the city of São Paulo still boast ample green spaces, offering oppor- tunities for natur e appr eciation and conservation, enhancing city resilience and livability. Citizen science is a collaborative endeavor between professional scientists and nonprofessional scientists in scientific research that may help to understand the dynamics of urban ecosystems. We believe citizen science has the potential to promote human and nature connection in urban areas and provide useful data on urban biodiversity. |
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Palavras-Chave: |
Alimentos silvestres; Árvores alimentares urbanas; Ciência cidadã; Citizen science; Floresta alimentar; Food forest; Forrageamento urbano; Fruit tree; Fruit-bearing plants; Plantas forrageiras urbanas; Plantas frutíferas; Urban food trees; Urban forageable plants; Urban foraging; Wild food. |
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Thesagro: |
Árvore Frutífera. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1163123/1/AP-Leveraging-citizen-science-2024.pdf
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Marc: |
LEADER 02311naa a2200565 a 4500
001 2163123
005 2024-03-25
008 2024 bl uuuu u00u1 u #d
024 7 $a10.1093/gigascience/giae007$2DOI
100 1 $aSOARES, F. M.
245 $aLeveraging citizen science for monitoring urban forageable plants.$h[electronic resource]
260 $c2024
520 $aUrbanization brings forth social challenges in emerging countries such as Brazil, encompassing food scarcity, health deterioration, air pollution, and biodi v ersity loss. Despite this, urban areas like the city of São Paulo still boast ample green spaces, offering oppor- tunities for natur e appr eciation and conservation, enhancing city resilience and livability. Citizen science is a collaborative endeavor between professional scientists and nonprofessional scientists in scientific research that may help to understand the dynamics of urban ecosystems. We believe citizen science has the potential to promote human and nature connection in urban areas and provide useful data on urban biodiversity.
650 $aÁrvore Frutífera
653 $aAlimentos silvestres
653 $aÁrvores alimentares urbanas
653 $aCiência cidadã
653 $aCitizen science
653 $aFloresta alimentar
653 $aFood forest
653 $aForrageamento urbano
653 $aFruit tree
653 $aFruit-bearing plants
653 $aPlantas forrageiras urbanas
653 $aPlantas frutíferas
653 $aUrban food trees
653 $aUrban forageable plants
653 $aUrban foraging
653 $aWild food
700 1 $aPIRES, L. F.
700 1 $aGARCIA, M. C.
700 1 $aBOUZEMBRAK, Y.
700 1 $aCORADIN, L.
700 1 $aGHILARDI-LOPES, N. P.
700 1 $aSILVA, R. R.
700 1 $aCARVALHO, A. M. de
700 1 $aMACULAN, B. C. M. dos S.
700 1 $aKOFFLER, S.
700 1 $aMONTEDO, U. B.
700 1 $aDRUCKER, D. P.
700 1 $aSANTIAGO, R.
700 1 $aGAVAI, A.
700 1 $aCARVALHO, M. C. P. de
700 1 $aLIMA, A. C. da S.
700 1 $aGABRIEL, H. D. E.
700 1 $aFRANÇA, S. G. M. de
700 1 $aALMEIDA, K. R. de
700 1 $aSANTOS, B. J. dos
700 1 $aSARAIVA, A. M.
773 $tGigaScience$gv. 13, n. 1, giae007, 2024
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Embrapa Agricultura Digital (CNPTIA) |
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