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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
23/11/2022 |
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Data da última atualização: |
13/02/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TEIXEIRA, T. S.; RODRIGUES NETO, J. C.; SILVA, E. A.; CONCEIÇÃO, A. A.; SIQUEIRA, F. G. de; ABDELNUR, P. V. |
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Afiliação: |
TALLYTA SANTOS TEIXEIRA, UNIVERSIDADE FEDERAL DE GOIÁS; JORGE CANDIDO RODRIGUES NETO, UNIVERSIDADE FEDERAL DE GOIÁS; ELIAS ALVES SILVA, CNPAE; APARECIDO ALMEIDA CONCEIÇÃO, CNPAE; FELIX GONCALVES DE SIQUEIRA, CNPAE; PATRICIA VERARDI ABDELNUR, CNPAE. |
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Título: |
Mass spectrometry imaging for fungal interaction analysis: Classic versus imprinting methods. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Brazilian Journal of Analytical Chemistry, v. 10, n. 38, p. 71-78, 2023. |
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DOI: |
10.30744/brjac.2179-3425.AR-44-2022 |
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Idioma: |
Inglês |
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Conteúdo: |
Fungi can produce many bioactive metabolites, which are enhanced when challenged in co-culture competition. For a better evaluation of these metabolites, Mass Spectrometry Imaging (MSI) can be used to provide complementary information about the metabolite spatial localization. However, some adaptations are required on available methodologies in MSI for applications in microorganisms, particularly on sample preparation, due to the characteristics of each type of cell that has to be analyzed. The imprinting method has been shown to be a robust method when applied to sample preparation, but to our knowledge it has never been tested for microbial MALDI-MSI. Herein we applied both Classic and Imprinting MALDIMSI to compare and analyze metabolites produced by Aspergillus terreus (ATCC® 20542TM) and Pleurotus pulmonarius fungi. For the classic method, the fungi were inoculated for 8 days with PDA medium in a MALDI glass slide. For the imprinting method, fungi were also inoculated for 8 days in a MALDI glass slide and then transferred to a filter paper by manual pressure using a homemade apparatus. Samples were then dehydrated and submitted to HCCA matrix application by sublimation. |
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Palavras-Chave: |
MALDI-MSI. |
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Thesagro: |
Biotecnologia. |
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Thesaurus Nal: |
Aspergillus terreus; Biotechnology; Metabolomics; Pleurotus pulmonarius. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/246602/1/Mass-spectrometry.pdf
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Marc: |
LEADER 02023naa a2200265 a 4500
001 2148633
005 2023-02-13
008 2023 bl uuuu u00u1 u #d
024 7 $a10.30744/brjac.2179-3425.AR-44-2022$2DOI
100 1 $aTEIXEIRA, T. S.
245 $aMass spectrometry imaging for fungal interaction analysis$bClassic versus imprinting methods.$h[electronic resource]
260 $c2023
520 $aFungi can produce many bioactive metabolites, which are enhanced when challenged in co-culture competition. For a better evaluation of these metabolites, Mass Spectrometry Imaging (MSI) can be used to provide complementary information about the metabolite spatial localization. However, some adaptations are required on available methodologies in MSI for applications in microorganisms, particularly on sample preparation, due to the characteristics of each type of cell that has to be analyzed. The imprinting method has been shown to be a robust method when applied to sample preparation, but to our knowledge it has never been tested for microbial MALDI-MSI. Herein we applied both Classic and Imprinting MALDIMSI to compare and analyze metabolites produced by Aspergillus terreus (ATCC® 20542TM) and Pleurotus pulmonarius fungi. For the classic method, the fungi were inoculated for 8 days with PDA medium in a MALDI glass slide. For the imprinting method, fungi were also inoculated for 8 days in a MALDI glass slide and then transferred to a filter paper by manual pressure using a homemade apparatus. Samples were then dehydrated and submitted to HCCA matrix application by sublimation.
650 $aAspergillus terreus
650 $aBiotechnology
650 $aMetabolomics
650 $aPleurotus pulmonarius
650 $aBiotecnologia
653 $aMALDI-MSI
700 1 $aRODRIGUES NETO, J. C.
700 1 $aSILVA, E. A.
700 1 $aCONCEIÇÃO, A. A.
700 1 $aSIQUEIRA, F. G. de
700 1 $aABDELNUR, P. V.
773 $tBrazilian Journal of Analytical Chemistry$gv. 10, n. 38, p. 71-78, 2023.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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| 1. |  | TEIXEIRA, T. S.; RODRIGUES NETO, J. C.; SILVA, E. A.; CONCEIÇÃO, A. A.; SIQUEIRA, F. G. de; ABDELNUR, P. V. Mass spectrometry imaging for fungal interaction analysis: Classic versus imprinting methods. Brazilian Journal of Analytical Chemistry, v. 10, n. 38, p. 71-78, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
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