| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Café. |
|
Data corrente: |
16/11/2022 |
|
Data da última atualização: |
16/11/2022 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
CASARIN, T.; FREITAS, N. C.; PINTO, R. T.; BREITLER, J. C.; RODRIGUES, L. A. Z.; MARRACCINI, P.; ETIENNE, H.; DINIZ, L. E. C.; ANDRADE, A. C.; PAIVA, L. V. |
|
Afiliação: |
TATIANE CASARIN, UNIVERSIDADE FEDERAL DE LAVRAS; NATÁLIA CHAGAS FREITAS, UNIVERSIDADE FEDERAL DE LAVRAS; RENAN TERASSI PINTO, UNIVERSIDADE FEDERAL DE LAVRAS; JEAN‐CHRISTOPHE BREITLER, CENTRO DE COOPERAÇÃO INTERNACIONAL EM PESQUISA AGRÍCOLA PARA O DESENVOLVIMENTO; LEONARDO AUGUSTO ZEBRAL RODRIGUES, UNIVERSIDADE FEDERAL DE LAVRAS; PIERRE MARRACCINI, CENTRO DE COOPERAÇÃO INTERNACIONAL EM PESQUISA AGRÍCOLA PARA O DESENVOLVIMENTO; HERVÉ ETIENNE, CENTRO DE COOPERAÇÃO INTERNACIONAL EM PESQUISA AGRÍCOLA PARA O DESENVOLVIMENTO; LEANDRO EUGENIO CARDAMONE DINIZ, CNPSO; ALAN CARVALHO ANDRADE, CNPCa; LUCIANO VILELA PAIVA, UNIVERSIDADE FEDERAL DE LAVRAS. |
|
Título: |
Multiplex CRISPR/Cas9-mediated knockout of the phytoene desaturase gene in Coffea canéfora. |
|
Ano de publicação: |
2022 |
|
Fonte/Imprenta: |
Scientific Reports, v. 12, 17270, 2022. 10 p. |
|
DOI: |
https://doi.org/10.1038/s41598-022-21566-w |
|
Idioma: |
Inglês |
|
Conteúdo: |
Coffea canephora (2n=2x22 chromosomes) is a species with extensive genetic diversity and desirable agronomic traits for coffee breeding programs. However, obtaining a new coffee cultivar through conventional breeding techniques may require more than 30 years of crossing cycles and selection, which hampers the effort of keeping up with market demands and rapidly proposing more resilient to climate change varieties. Although, the application of modern biotechnology tools such as precision genetic engineering technologies may enable a faster cultivar development process. Therefore, we aimed to validate the CRISPR/Cas9 system to generate mutations on a selected genotype of C. canephora, the clone 14. Embryogenic calli and a multiplex binary vector containing two sgRNAs targeting different exons of the CcPDS gene were used. The sgRNAs were under the C. canephora U6 promoter regulation. The target gene encodes phytoene desaturase, an enzyme essential for photosynthesis involved in B-carotene biosynthesis. Somatic seedlings and embryos with albino, variegated and green phenotypes regenerated after Agrobacterium tumefaciens-mediated genetic transformation were analyzed by verifying the insertion of the Cas9 gene and later by sequencing the sgRNAs target regions in the genome of Robusta modified seedlings. Among them, 77% had the expected mutations, and of which, 50% of them had at least one target with a homozygous mutation. The genotype, temperature of co-cultivation with the bacteria, and light intensity used for subsequent embryo regeneration appeared to strongly influence the successful regeneration of plants with a mutated CcPDS gene in the Coffea genus. MenosCoffea canephora (2n=2x22 chromosomes) is a species with extensive genetic diversity and desirable agronomic traits for coffee breeding programs. However, obtaining a new coffee cultivar through conventional breeding techniques may require more than 30 years of crossing cycles and selection, which hampers the effort of keeping up with market demands and rapidly proposing more resilient to climate change varieties. Although, the application of modern biotechnology tools such as precision genetic engineering technologies may enable a faster cultivar development process. Therefore, we aimed to validate the CRISPR/Cas9 system to generate mutations on a selected genotype of C. canephora, the clone 14. Embryogenic calli and a multiplex binary vector containing two sgRNAs targeting different exons of the CcPDS gene were used. The sgRNAs were under the C. canephora U6 promoter regulation. The target gene encodes phytoene desaturase, an enzyme essential for photosynthesis involved in B-carotene biosynthesis. Somatic seedlings and embryos with albino, variegated and green phenotypes regenerated after Agrobacterium tumefaciens-mediated genetic transformation were analyzed by verifying the insertion of the Cas9 gene and later by sequencing the sgRNAs target regions in the genome of Robusta modified seedlings. Among them, 77% had the expected mutations, and of which, 50% of them had at least one target with a homozygous mutation. The genotype, temperature of co-cultivation with the bacte... Mostrar Tudo |
|
Thesagro: |
Biodiversidade; Café Robusta; Coffea Canephora; Genótipo; Melhoramento Genético Vegetal; Mutação. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148303/1/Multiplex-CRISPRCas9-mediated.pdf
|
|
Marc: |
LEADER 02597naa a2200313 a 4500
001 2148303
005 2022-11-16
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1038/s41598-022-21566-w$2DOI
100 1 $aCASARIN, T.
245 $aMultiplex CRISPR/Cas9-mediated knockout of the phytoene desaturase gene in Coffea canéfora.$h[electronic resource]
260 $c2022
520 $aCoffea canephora (2n=2x22 chromosomes) is a species with extensive genetic diversity and desirable agronomic traits for coffee breeding programs. However, obtaining a new coffee cultivar through conventional breeding techniques may require more than 30 years of crossing cycles and selection, which hampers the effort of keeping up with market demands and rapidly proposing more resilient to climate change varieties. Although, the application of modern biotechnology tools such as precision genetic engineering technologies may enable a faster cultivar development process. Therefore, we aimed to validate the CRISPR/Cas9 system to generate mutations on a selected genotype of C. canephora, the clone 14. Embryogenic calli and a multiplex binary vector containing two sgRNAs targeting different exons of the CcPDS gene were used. The sgRNAs were under the C. canephora U6 promoter regulation. The target gene encodes phytoene desaturase, an enzyme essential for photosynthesis involved in B-carotene biosynthesis. Somatic seedlings and embryos with albino, variegated and green phenotypes regenerated after Agrobacterium tumefaciens-mediated genetic transformation were analyzed by verifying the insertion of the Cas9 gene and later by sequencing the sgRNAs target regions in the genome of Robusta modified seedlings. Among them, 77% had the expected mutations, and of which, 50% of them had at least one target with a homozygous mutation. The genotype, temperature of co-cultivation with the bacteria, and light intensity used for subsequent embryo regeneration appeared to strongly influence the successful regeneration of plants with a mutated CcPDS gene in the Coffea genus.
650 $aBiodiversidade
650 $aCafé Robusta
650 $aCoffea Canephora
650 $aGenótipo
650 $aMelhoramento Genético Vegetal
650 $aMutação
700 1 $aFREITAS, N. C.
700 1 $aPINTO, R. T.
700 1 $aBREITLER, J. C.
700 1 $aRODRIGUES, L. A. Z.
700 1 $aMARRACCINI, P.
700 1 $aETIENNE, H.
700 1 $aDINIZ, L. E. C.
700 1 $aANDRADE, A. C.
700 1 $aPAIVA, L. V.
773 $tScientific Reports$gv. 12, 17270, 2022. 10 p.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Café (CNPCa) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Solos. |
|
Data corrente: |
03/08/2020 |
|
Data da última atualização: |
03/08/2020 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
VIEIRA, I. F. B.; ROLIM NETO, F. C.; CARVALHO, M. N.; CALDAS, A. M.; COSTA, R. C. A.; SILVA, K. S. da; PARAHYBA, R. da B. V.; PACHECO, F. A. L.; FERNANDES, L. F. S.; PISSARRA, T. C. T. |
|
Afiliação: |
IGOR FELLIPE BATISTA VIEIRA, UFRPE; FERNANDO CARTAXO ROLIM NETO, UFRPE; MARILDA NASCIMENTO CARVALHO, UFPE; ANILDO MONTEIRO CALDAS, UFRPE; RENATA CRISTINA ARAÚJO COSTA, UNESP/FCAV; KAROLYNE SANTOS DA SILVA, UFPE; ROBERTO DA BOA VIAGEM PARAHYBA, CNPS; FERNANDO ANTONIO LEAL PACHECO, University of Trás-os-Montes and Alto Douro; LUÍS FILIPE SANCHES FERNANDES, University of Trás-os-Montes and Alto Douro; TERESA CRISTINA TARLÉ PISSARRA, UNESP/FCAV. |
|
Título: |
Water security assessment of groundwater quality in an anthropized rural area from the Atlantic Forest Biome in Brazil. |
|
Ano de publicação: |
2020 |
|
Fonte/Imprenta: |
Water, v. 12, n. 3, 623, 2020. |
|
DOI: |
https://doi.org/10.3390/w12030623 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The exploitation of natural resources has grown mainly due to the high rate of population growth that changed over time around the planet. Water is one of the most needed resources essential for survival. Despite all the efforts made to improve water security, an environmental impact related to anthropogenic influence remains of great concern, which is the alteration of surface and groundwater quality. In many regions around the world, there is limited or no access to rural and urban water supply while there is a need to improve sanitation facilities. This work evaluated the spatial distribution of groundwater and surface water quality as well as their changes in wet and dry seasons of the tropical climate in the Atlantic Forest Biome. The study area is under anthropogenic influence, which is in the municipality of Igarassú, Pernambuco State, Brazil. The analysis of the raw water was based on Standard Methods for Examination of Water and Wastewater, as referenced in the Brazilian Ministry of Health Consolidation Ordinance that sets standards for drinking water. The temporal analyses indicated a variation on water quality from the wet to the dry seasons, whereas the spatial results revealed deviations from the Brazilian's Water Supply Standards for some physicochemical parameters. There was an increase in the values of some parameters during the wet season in some hydrological compartments. The anthropized rural area from the Atlantic Forest Biome is affecting the water quality. It is, therefore, necessary to develop environmental policies and put them into practice by implementing engineering projects that guarantee proper treatment for raw water in order to bring the water quality back to a good status in this region. MenosThe exploitation of natural resources has grown mainly due to the high rate of population growth that changed over time around the planet. Water is one of the most needed resources essential for survival. Despite all the efforts made to improve water security, an environmental impact related to anthropogenic influence remains of great concern, which is the alteration of surface and groundwater quality. In many regions around the world, there is limited or no access to rural and urban water supply while there is a need to improve sanitation facilities. This work evaluated the spatial distribution of groundwater and surface water quality as well as their changes in wet and dry seasons of the tropical climate in the Atlantic Forest Biome. The study area is under anthropogenic influence, which is in the municipality of Igarassú, Pernambuco State, Brazil. The analysis of the raw water was based on Standard Methods for Examination of Water and Wastewater, as referenced in the Brazilian Ministry of Health Consolidation Ordinance that sets standards for drinking water. The temporal analyses indicated a variation on water quality from the wet to the dry seasons, whereas the spatial results revealed deviations from the Brazilian's Water Supply Standards for some physicochemical parameters. There was an increase in the values of some parameters during the wet season in some hydrological compartments. The anthropized rural area from the Atlantic Forest Biome is affecting the water quali... Mostrar Tudo |
|
Thesagro: |
Água Potável; Lençol Freático; Qualidade da Água. |
|
Thesaurus NAL: |
Drinking water; Environmental monitoring; Groundwater; Surface water; Water quality. |
|
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/215082/1/Water-security-assessment-of-groundwater-quality-in-an-anthropized-rural-area-from-the-Atlantic-Forest-Biome-in-Brazil-2020.pdf
|
|
Marc: |
LEADER 02765naa a2200337 a 4500
001 2124163
005 2020-08-03
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/w12030623$2DOI
100 1 $aVIEIRA, I. F. B.
245 $aWater security assessment of groundwater quality in an anthropized rural area from the Atlantic Forest Biome in Brazil.$h[electronic resource]
260 $c2020
520 $aThe exploitation of natural resources has grown mainly due to the high rate of population growth that changed over time around the planet. Water is one of the most needed resources essential for survival. Despite all the efforts made to improve water security, an environmental impact related to anthropogenic influence remains of great concern, which is the alteration of surface and groundwater quality. In many regions around the world, there is limited or no access to rural and urban water supply while there is a need to improve sanitation facilities. This work evaluated the spatial distribution of groundwater and surface water quality as well as their changes in wet and dry seasons of the tropical climate in the Atlantic Forest Biome. The study area is under anthropogenic influence, which is in the municipality of Igarassú, Pernambuco State, Brazil. The analysis of the raw water was based on Standard Methods for Examination of Water and Wastewater, as referenced in the Brazilian Ministry of Health Consolidation Ordinance that sets standards for drinking water. The temporal analyses indicated a variation on water quality from the wet to the dry seasons, whereas the spatial results revealed deviations from the Brazilian's Water Supply Standards for some physicochemical parameters. There was an increase in the values of some parameters during the wet season in some hydrological compartments. The anthropized rural area from the Atlantic Forest Biome is affecting the water quality. It is, therefore, necessary to develop environmental policies and put them into practice by implementing engineering projects that guarantee proper treatment for raw water in order to bring the water quality back to a good status in this region.
650 $aDrinking water
650 $aEnvironmental monitoring
650 $aGroundwater
650 $aSurface water
650 $aWater quality
650 $aÁgua Potável
650 $aLençol Freático
650 $aQualidade da Água
700 1 $aROLIM NETO, F. C.
700 1 $aCARVALHO, M. N.
700 1 $aCALDAS, A. M.
700 1 $aCOSTA, R. C. A.
700 1 $aSILVA, K. S. da
700 1 $aPARAHYBA, R. da B. V.
700 1 $aPACHECO, F. A. L.
700 1 $aFERNANDES, L. F. S.
700 1 $aPISSARRA, T. C. T
773 $tWater$gv. 12, n. 3, 623, 2020.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Solos (CNPS) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
