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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
21/10/2022 |
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Data da última atualização: |
30/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARVALHO, T. G.; ROSMAN, A. C.; GRAVITOL, C.; NOGUEIRA, E. de M.; BALDANI, J. I.; HEMERLY, A. S. |
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Afiliação: |
THAIS LOUISE G. CARVALHO, UFRJ; ALINE CARDOZO ROSMAN, UFRJ; CLICIA GRATIVOL, UENF; EDUARDO DE M. NOGUEIRA, UFRJ; JOSE IVO BALDANI, CNPAB; ADRIANA S. HEMERLY, UFRJ. |
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Título: |
genotypes with contrasting biological nitrogen fixation efficiencies differentially modulate nitrogen metabolism, auxin signaling, and microorganism perception pathways. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Plants, v. 11, n. 15, 1971, 2022. |
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ISSN: |
2223-7747 |
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Idioma: |
Inglês |
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Conteúdo: |
Sugarcane is an economically important crop that is used for the production of fuel ethanol. Diazotrophic bacteria have been isolated from sugarcane tissues, without causing visible plant anatomical changes or disease symptoms. These bacteria can be beneficial to the plant by promoting root growth and an increase in plant yield. Different rates of Biological Nitrogen Fixation (BNF) were observed in different genotypes. The aim of this work was to conduct a comprehensive molecular and physiological analysis of two model genotypes for contrasting BNF efficiency in order to unravel plant genes that are differentially regulated during a natural association with diazotrophic bacteria. A next-generation sequencing of RNA samples from the genotypes SP70-1143 (high-BNF) and Chunee (low-BNF) was performed. A differential transcriptome analysis showed that several pathways were differentially regulated among the two BNF-contrasting genotypes, including nitrogen metabolism, hormone regulation and bacteria recognition. Physiological analyses, such as nitrogenase and GS activity quantification, bacterial colonization, auxin response and root architecture evaluation, supported the transcriptome expression analyses. The differences observed between the genotypes may explain, at least in part, the differences in BNF contributions. Some of the identified genes might be involved in key regulatory processes for a beneficial association and could be further used as tools for obtaining more efficient BNF genotypes. MenosSugarcane is an economically important crop that is used for the production of fuel ethanol. Diazotrophic bacteria have been isolated from sugarcane tissues, without causing visible plant anatomical changes or disease symptoms. These bacteria can be beneficial to the plant by promoting root growth and an increase in plant yield. Different rates of Biological Nitrogen Fixation (BNF) were observed in different genotypes. The aim of this work was to conduct a comprehensive molecular and physiological analysis of two model genotypes for contrasting BNF efficiency in order to unravel plant genes that are differentially regulated during a natural association with diazotrophic bacteria. A next-generation sequencing of RNA samples from the genotypes SP70-1143 (high-BNF) and Chunee (low-BNF) was performed. A differential transcriptome analysis showed that several pathways were differentially regulated among the two BNF-contrasting genotypes, including nitrogen metabolism, hormone regulation and bacteria recognition. Physiological analyses, such as nitrogenase and GS activity quantification, bacterial colonization, auxin response and root architecture evaluation, supported the transcriptome expression analyses. The differences observed between the genotypes may explain, at least in part, the differences in BNF contributions. Some of the identified genes might be involved in key regulatory processes for a beneficial association and could be further used as tools for obtaining more effi... Mostrar Tudo |
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Palavras-Chave: |
Differential RNA seq; Endophytic diazotrophic bacteria; Nitrogen fixing bacteria. |
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Thesaurus Nal: |
Nitrogen metabolism; Transcriptome. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1147564/1/Sugarcane-Genotypes-with-Contrasting-Biological.pdf
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Marc: |
LEADER 02345naa a2200253 a 4500
001 2147564
005 2022-11-30
008 2022 bl uuuu u00u1 u #d
022 $a2223-7747
100 1 $aCARVALHO, T. G.
245 $agenotypes with contrasting biological nitrogen fixation efficiencies differentially modulate nitrogen metabolism, auxin signaling, and microorganism perception pathways.$h[electronic resource]
260 $c2022
520 $aSugarcane is an economically important crop that is used for the production of fuel ethanol. Diazotrophic bacteria have been isolated from sugarcane tissues, without causing visible plant anatomical changes or disease symptoms. These bacteria can be beneficial to the plant by promoting root growth and an increase in plant yield. Different rates of Biological Nitrogen Fixation (BNF) were observed in different genotypes. The aim of this work was to conduct a comprehensive molecular and physiological analysis of two model genotypes for contrasting BNF efficiency in order to unravel plant genes that are differentially regulated during a natural association with diazotrophic bacteria. A next-generation sequencing of RNA samples from the genotypes SP70-1143 (high-BNF) and Chunee (low-BNF) was performed. A differential transcriptome analysis showed that several pathways were differentially regulated among the two BNF-contrasting genotypes, including nitrogen metabolism, hormone regulation and bacteria recognition. Physiological analyses, such as nitrogenase and GS activity quantification, bacterial colonization, auxin response and root architecture evaluation, supported the transcriptome expression analyses. The differences observed between the genotypes may explain, at least in part, the differences in BNF contributions. Some of the identified genes might be involved in key regulatory processes for a beneficial association and could be further used as tools for obtaining more efficient BNF genotypes.
650 $aNitrogen metabolism
650 $aTranscriptome
653 $aDifferential RNA seq
653 $aEndophytic diazotrophic bacteria
653 $aNitrogen fixing bacteria
700 1 $aROSMAN, A. C.
700 1 $aGRAVITOL, C.
700 1 $aNOGUEIRA, E. de M.
700 1 $aBALDANI, J. I.
700 1 $aHEMERLY, A. S.
773 $tPlants$gv. 11, n. 15, 1971, 2022.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| 1. |  | CARVALHO, T. G.; ROSMAN, A. C.; GRAVITOL, C.; NOGUEIRA, E. de M.; BALDANI, J. I.; HEMERLY, A. S. genotypes with contrasting biological nitrogen fixation efficiencies differentially modulate nitrogen metabolism, auxin signaling, and microorganism perception pathways. Plants, v. 11, n. 15, 1971, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Agrobiologia. |
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