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Registro Completo |
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
20/09/2022 |
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Data da última atualização: |
05/02/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SYBUIA, P. A.; CASTRO-PRADO, G. de; NUNES, W. M. de C.; ZANUTTO, C. A.; KANESHIMA, E. N.; SOARES, D. J.; FRANCO, C. C. da S.; MATHIAS, P. C. de F.; CASTRO-PRADO, M. A. A. de. |
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Afiliação: |
PRISCILA AYUMI SYBUIA, UEM; GABRIELA DE CASTRO-PRADO, UEM; WILLIAM MÁRIO DE CARVALHO NUNES, UEM; CARLOS ALEXANDRE ZANUTTO, UEM; EDILSON NOBUYOSHI KANESHIMA, UEM; DARTANHÃ JOSÉ SOARES, CNPA; CLAUDINÉIA CONATIONI DA SILVA FRANCO, UEM; PAULO CEZAR DE FREITAS MATHIAS, UEM; MARIALBA AVEZUM ALVES DE CASTRO-PRADO, UEM. |
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Título: |
Parasexual recombination in Macrophomina pseudophaseolina and vegetative compatibility reactions in M. euphorbiicola. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
European Journal of Plant Pathology, v. 163, p. 937-950, 2022. |
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ISSN: |
0929-1873 |
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DOI: |
https://doi.org/10.1007/s10658-022-02530-w |
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Idioma: |
Português |
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Conteúdo: |
Charcoal rot is an economically important fungal disease of economically important crops. Several species of the Macrophomina genus, such as M. pseudophaseolina and M. euphorbiicola, may act as the causal agents of charcoal rot. The current study evaluates: a) the diversity of vegetative compatibility reactions in Brazilian isolates of M. pseudophaseolina (totalling 27) and M. euphorbiicola (totalling 3), b) the genetic variability of Brazilian isolates, and c) the occurrence of parasexual recombination in M. pseudophaseolina isolates. In complementation tests, 2 and 18 Vegetative Compatibility Groups (VCGs) were identified for the M. euphorbiicola and M. pseudophaseolina isolates, respectively. Correlations between VCG and the isolates´geographic origin were not found since most isolates derived from the same localities were grouped in distinct VCGs, demonstrating their genetic variability. Further, RAPD analysis of the isolates did not reveal significant correlations between genotype, geographic location and hosts, since isolates derived from distinct localities or hosts formed the same RAPD group. Heterozygous diploids were produced as fast-growing sectors by heterokaryons formed with M. pseudophaseolina isolates, demonstrating, for the first time, the occurrence of the parasexual cycle in the species, based on molecular and phenotypic evidence. Diploids spontaneously produced paternal segregants and parasexual recombinants, demonstrating that parasexuality is an important mechanism for transferring genetic material in filamentous fungi. Results suggest that parasexual recombination may play an important role in the genetic variability of M. pseudophaseolina and may be the cause for the origin of new pathotypes, which could compromise disease control strategies in crops. MenosCharcoal rot is an economically important fungal disease of economically important crops. Several species of the Macrophomina genus, such as M. pseudophaseolina and M. euphorbiicola, may act as the causal agents of charcoal rot. The current study evaluates: a) the diversity of vegetative compatibility reactions in Brazilian isolates of M. pseudophaseolina (totalling 27) and M. euphorbiicola (totalling 3), b) the genetic variability of Brazilian isolates, and c) the occurrence of parasexual recombination in M. pseudophaseolina isolates. In complementation tests, 2 and 18 Vegetative Compatibility Groups (VCGs) were identified for the M. euphorbiicola and M. pseudophaseolina isolates, respectively. Correlations between VCG and the isolates´geographic origin were not found since most isolates derived from the same localities were grouped in distinct VCGs, demonstrating their genetic variability. Further, RAPD analysis of the isolates did not reveal significant correlations between genotype, geographic location and hosts, since isolates derived from distinct localities or hosts formed the same RAPD group. Heterozygous diploids were produced as fast-growing sectors by heterokaryons formed with M. pseudophaseolina isolates, demonstrating, for the first time, the occurrence of the parasexual cycle in the species, based on molecular and phenotypic evidence. Diploids spontaneously produced paternal segregants and parasexual recombinants, demonstrating that parasexuality is an importan... Mostrar Tudo |
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Palavras-Chave: |
HSI phenotype; Parasexual recombination; RAPD/PCR. |
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Thesagro: |
Doença Fúngica. |
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Thesaurus Nal: |
Charcoal rot; Fungal diseases of plants; Parasexual cycle. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02834naa a2200325 a 4500
001 2146645
005 2023-02-05
008 2022 bl uuuu u00u1 u #d
022 $a0929-1873
024 7 $ahttps://doi.org/10.1007/s10658-022-02530-w$2DOI
100 1 $aSYBUIA, P. A.
245 $aParasexual recombination in Macrophomina pseudophaseolina and vegetative compatibility reactions in M. euphorbiicola.$h[electronic resource]
260 $c2022
520 $aCharcoal rot is an economically important fungal disease of economically important crops. Several species of the Macrophomina genus, such as M. pseudophaseolina and M. euphorbiicola, may act as the causal agents of charcoal rot. The current study evaluates: a) the diversity of vegetative compatibility reactions in Brazilian isolates of M. pseudophaseolina (totalling 27) and M. euphorbiicola (totalling 3), b) the genetic variability of Brazilian isolates, and c) the occurrence of parasexual recombination in M. pseudophaseolina isolates. In complementation tests, 2 and 18 Vegetative Compatibility Groups (VCGs) were identified for the M. euphorbiicola and M. pseudophaseolina isolates, respectively. Correlations between VCG and the isolates´geographic origin were not found since most isolates derived from the same localities were grouped in distinct VCGs, demonstrating their genetic variability. Further, RAPD analysis of the isolates did not reveal significant correlations between genotype, geographic location and hosts, since isolates derived from distinct localities or hosts formed the same RAPD group. Heterozygous diploids were produced as fast-growing sectors by heterokaryons formed with M. pseudophaseolina isolates, demonstrating, for the first time, the occurrence of the parasexual cycle in the species, based on molecular and phenotypic evidence. Diploids spontaneously produced paternal segregants and parasexual recombinants, demonstrating that parasexuality is an important mechanism for transferring genetic material in filamentous fungi. Results suggest that parasexual recombination may play an important role in the genetic variability of M. pseudophaseolina and may be the cause for the origin of new pathotypes, which could compromise disease control strategies in crops.
650 $aCharcoal rot
650 $aFungal diseases of plants
650 $aParasexual cycle
650 $aDoença Fúngica
653 $aHSI phenotype
653 $aParasexual recombination
653 $aRAPD/PCR
700 1 $aCASTRO-PRADO, G. de
700 1 $aNUNES, W. M. de C.
700 1 $aZANUTTO, C. A.
700 1 $aKANESHIMA, E. N.
700 1 $aSOARES, D. J.
700 1 $aFRANCO, C. C. da S.
700 1 $aMATHIAS, P. C. de F.
700 1 $aCASTRO-PRADO, M. A. A. de
773 $tEuropean Journal of Plant Pathology$gv. 163, p. 937-950, 2022.
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| 1. |  | SYBUIA, P. A.; CASTRO-PRADO, G. de; NUNES, W. M. de C.; ZANUTTO, C. A.; KANESHIMA, E. N.; SOARES, D. J.; FRANCO, C. C. da S.; MATHIAS, P. C. de F.; CASTRO-PRADO, M. A. A. de. Parasexual recombination in Macrophomina pseudophaseolina and vegetative compatibility reactions in M. euphorbiicola. European Journal of Plant Pathology, v. 163, p. 937-950, 2022.| Tipo: Artigo em Periódico Indexado |
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