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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
25/07/2022 |
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Data da última atualização: |
25/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OKINO, C. H.; MALAGO JUNIOR, W.; MARCONDES, C. R.; GIGLIOTI, R.; MONTASSIER, H. J.; OLIVEIRA, H. N. DE; OLIVEIRA, M. C. de S. |
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Afiliação: |
CINTIA HIROMI OKINO, CPPSE; WILSON MALAGO JUNIOR, CPPSE; CINTIA RIGHETTI MARCONDES, CPPSE; RODRIGO GIGLIOTI, Centro de Pesquisa e Desenvolvimento de Genética e Biotecnologia, Instituto de Zootecnia (IZ), Nova Odessa; HÉLIO JOSÉ MONTASSIER, UNESP; HENRIQUE NUNES DE OLIVEIRA, UNESP; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE. |
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Título: |
CD4 bovine gene: differential polymorphisms among cattle breeds and a new tool for rapid identification. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Veterinary Immunology and Immunopathology, v. 251, sep. 2022, 110462. |
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Páginas: |
18 p. |
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DOI: |
ttps://doi.org/10.1016/j.vetimm.2022.110462 |
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Idioma: |
Inglês |
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Conteúdo: |
Two mutations in the CD4 bovine gene (G>T/Q306H; A>C/K310N) were identified as causative for altered staining with anti-CD4 mAb #CC8. We developed a HRM qPCR for genotyping these mutations and compare with immunophenotyping in different cattle breeds. The assay distinguished five genotypes, B (homozygous, G/A) and C (heterozygous, G/A and T/C), found in taurine, A (homozygous, G/C) and D (heterozygous, T/C and G/C), found in zebu. The E genotype (homozygous, T/C) was not observed in tested animals. As expected, B and C presented high/very high and intermediate CD4 staining, respectively. The lack/low CD4 staining was mainly related to the A, while the intermediate staining was mainly related to D genotype. The developed HRM qPCR assay accurately identified the altered phenotypes associated with CC8 staining in taurine. However, the assay cannot be applicable in zebu or hybrid breeds, probably due to additional mutations in the CD4 gene from zebu descendant animals. |
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Palavras-Chave: |
High Resolution Melt; Immunophenotyping; QPCR; T helper cells. |
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Thesaurus Nal: |
Genotype. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/237927/1/CD4-Bovine-Gene.pdf
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Marc: |
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024 7 $attps://doi.org/10.1016/j.vetimm.2022.110462$2DOI
100 1 $aOKINO, C. H.
245 $aCD4 bovine gene$bdifferential polymorphisms among cattle breeds and a new tool for rapid identification.$h[electronic resource]
260 $c2022
300 $a18 p.
520 $aTwo mutations in the CD4 bovine gene (G>T/Q306H; A>C/K310N) were identified as causative for altered staining with anti-CD4 mAb #CC8. We developed a HRM qPCR for genotyping these mutations and compare with immunophenotyping in different cattle breeds. The assay distinguished five genotypes, B (homozygous, G/A) and C (heterozygous, G/A and T/C), found in taurine, A (homozygous, G/C) and D (heterozygous, T/C and G/C), found in zebu. The E genotype (homozygous, T/C) was not observed in tested animals. As expected, B and C presented high/very high and intermediate CD4 staining, respectively. The lack/low CD4 staining was mainly related to the A, while the intermediate staining was mainly related to D genotype. The developed HRM qPCR assay accurately identified the altered phenotypes associated with CC8 staining in taurine. However, the assay cannot be applicable in zebu or hybrid breeds, probably due to additional mutations in the CD4 gene from zebu descendant animals.
650 $aGenotype
653 $aHigh Resolution Melt
653 $aImmunophenotyping
653 $aQPCR
653 $aT helper cells
700 1 $aMALAGO JUNIOR, W.
700 1 $aMARCONDES, C. R.
700 1 $aGIGLIOTI, R.
700 1 $aMONTASSIER, H. J.
700 1 $aOLIVEIRA, H. N. DE
700 1 $aOLIVEIRA, M. C. de S.
773 $tVeterinary Immunology and Immunopathology$gv. 251, sep. 2022, 110462.
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Embrapa Pecuária Sudeste (CPPSE) |
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| 1. |  | OKINO, C. H.; MALAGO JUNIOR, W.; MARCONDES, C. R.; GIGLIOTI, R.; MONTASSIER, H. J.; OLIVEIRA, H. N. DE; OLIVEIRA, M. C. de S. CD4 bovine gene: differential polymorphisms among cattle breeds and a new tool for rapid identification. Veterinary Immunology and Immunopathology, v. 251, sep. 2022, 110462. 18 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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