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Registro Completo |
Biblioteca(s): |
Embrapa Pantanal. |
Data corrente: |
12/07/2022 |
Data da última atualização: |
03/01/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
BRANDÃO, M. da S.; MUNIZ, F.; HERNÁNDEZ-RANGEL, S.; CAMPOS, Z.; FARIAS, I.; HRBEK, T. |
Afiliação: |
MAYNARA DA SILVA BRANDÃO, Federal University of Amazonas, UFAM, Manaus; FÁBIO MUNIZ, Federal University of Amazonas, UFAM, Manaus; SANDRA HERNÁNDEZ-RANGEL, Federal University of Amazonas, UFAM, Manaus; ZILCA MARIA DA SILVA CAMPOS, CPAP; IZENI FARIAS, Federal University of Amazonas, UFAM, Manaus; TOMAS HRBEK, Federal University of Amazonas, UFAM, Manaus. |
Título: |
SNPs marker toolbox for molecular identification of nominal and candidate species of the genus Paleosuchus. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
In: WORKING MEETING OF THE CROCODILE SPECIALIST GROUP OF THE INTERNATIONAL UNION FOR CONSERVATION NATURE, 26., 2022, Chetumal. Abstracts... [Chetumal]: CSG, 2022. |
Páginas: |
p. 123. |
Idioma: |
Inglês |
Notas: |
Evento Online. |
Conteúdo: |
Recent studies have identified the existence of species complexes in crocodilians, including in the New World Caimaninae. Both species of the genus Paleosuchus are species complexes with three or four species subsumed under the P. palpebrosus epithet and two under the P. trigonatus epithet. From the conservation point of view, it is important to recognize the evolutionary and ecological uniqueness of these lineages, regardless of the taxonomic status they may receive. Candidate species of the genus Paleosuchus have not yet been formally described and therefore do not have diagnostic morphological characters that would allow their identification in nature. This problem motivated us to develop a SNP (Single Nucleotide Polymorphisms) toolkit that enables molecular identification of the nominal and candidate species of Paleosuchus. For this, a ddRADseq (Double Digest RAD-sequencing) protocol was used to obtain reduced representation genome of 20 P. palpebrosus samples, five from each of four lineages ('Amazonia', 'Bolivia', 'Madeira' and 'Pantanal'), and five samples of P. trigonatus, three from the 'Amazon' and two from the 'Guyana' lineage. We selected from between 11 and 15 private SNPs for each lineage that unambiguously assign individuals to one of these lineages. Additionally, we selected 11 mutually exclusive private SNPs that unambiguously assign individuals to one of the two species complexes. The use of the toolkit is affordable and fast-to-apply in comparison to other molecular identification methods, and so it will enable a rapid molecular assignment of individuals to the newly identified lineages of the genus, which may be suffering different degrees of threat. Additional applications of this marker set are: the assignment of zoo specimens, pet trade individuals, museum samples, or degraded samples to source lineages; molecular identification seized animal parts (eg. bush meat); and identification of the origin of illegally traded animals or their by-products (forensic genetics). MenosRecent studies have identified the existence of species complexes in crocodilians, including in the New World Caimaninae. Both species of the genus Paleosuchus are species complexes with three or four species subsumed under the P. palpebrosus epithet and two under the P. trigonatus epithet. From the conservation point of view, it is important to recognize the evolutionary and ecological uniqueness of these lineages, regardless of the taxonomic status they may receive. Candidate species of the genus Paleosuchus have not yet been formally described and therefore do not have diagnostic morphological characters that would allow their identification in nature. This problem motivated us to develop a SNP (Single Nucleotide Polymorphisms) toolkit that enables molecular identification of the nominal and candidate species of Paleosuchus. For this, a ddRADseq (Double Digest RAD-sequencing) protocol was used to obtain reduced representation genome of 20 P. palpebrosus samples, five from each of four lineages ('Amazonia', 'Bolivia', 'Madeira' and 'Pantanal'), and five samples of P. trigonatus, three from the 'Amazon' and two from the 'Guyana' lineage. We selected from between 11 and 15 private SNPs for each lineage that unambiguously assign individuals to one of these lineages. Additionally, we selected 11 mutually exclusive private SNPs that unambiguously assign individuals to one of the two species complexes. The use of the toolkit is affordable and fast-to-apply in comparison to other... Mostrar Tudo |
Thesagro: |
Jacaré. |
Thesaurus Nal: |
Caiman. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1144666/1/CSG26-Abstracts-Program-220707-151117-123.pdf
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Marc: |
LEADER 02768nam a2200217 a 4500 001 2144666 005 2023-01-03 008 2022 bl uuuu u00u1 u #d 100 1 $aBRANDÃO, M. da S. 245 $aSNPs marker toolbox for molecular identification of nominal and candidate species of the genus Paleosuchus.$h[electronic resource] 260 $aIn: WORKING MEETING OF THE CROCODILE SPECIALIST GROUP OF THE INTERNATIONAL UNION FOR CONSERVATION NATURE, 26., 2022, Chetumal. Abstracts... [Chetumal]: CSG$c2022 300 $ap. 123. 500 $aEvento Online. 520 $aRecent studies have identified the existence of species complexes in crocodilians, including in the New World Caimaninae. Both species of the genus Paleosuchus are species complexes with three or four species subsumed under the P. palpebrosus epithet and two under the P. trigonatus epithet. From the conservation point of view, it is important to recognize the evolutionary and ecological uniqueness of these lineages, regardless of the taxonomic status they may receive. Candidate species of the genus Paleosuchus have not yet been formally described and therefore do not have diagnostic morphological characters that would allow their identification in nature. This problem motivated us to develop a SNP (Single Nucleotide Polymorphisms) toolkit that enables molecular identification of the nominal and candidate species of Paleosuchus. For this, a ddRADseq (Double Digest RAD-sequencing) protocol was used to obtain reduced representation genome of 20 P. palpebrosus samples, five from each of four lineages ('Amazonia', 'Bolivia', 'Madeira' and 'Pantanal'), and five samples of P. trigonatus, three from the 'Amazon' and two from the 'Guyana' lineage. We selected from between 11 and 15 private SNPs for each lineage that unambiguously assign individuals to one of these lineages. Additionally, we selected 11 mutually exclusive private SNPs that unambiguously assign individuals to one of the two species complexes. The use of the toolkit is affordable and fast-to-apply in comparison to other molecular identification methods, and so it will enable a rapid molecular assignment of individuals to the newly identified lineages of the genus, which may be suffering different degrees of threat. Additional applications of this marker set are: the assignment of zoo specimens, pet trade individuals, museum samples, or degraded samples to source lineages; molecular identification seized animal parts (eg. bush meat); and identification of the origin of illegally traded animals or their by-products (forensic genetics). 650 $aCaiman 650 $aJacaré 700 1 $aMUNIZ, F. 700 1 $aHERNÁNDEZ-RANGEL, S. 700 1 $aCAMPOS, Z. 700 1 $aFARIAS, I. 700 1 $aHRBEK, T.
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Embrapa Pantanal (CPAP) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Cerrados; Embrapa Soja. |
Data corrente: |
10/04/2000 |
Data da última atualização: |
14/08/2006 |
Autoria: |
SOUZA, P. I. de M.; ARANTES, N. E.; FARIA, L. C. de. |
Título: |
Parceria tecnica entre os convenios da Embrapa em Minas Gerais, Goias e Embrapa Cerrados. |
Ano de publicação: |
1999 |
Fonte/Imprenta: |
In: REUNIAO DE PESQUISA DE SOJA DA REGIAO CENTRAL DO BRASIL, 21., 1999, Dourados. Resumos... Dourados: Embrapa Agropecuaria Oeste / Londrina: Embrapa Soja, 1999. |
Páginas: |
p.177. |
Série: |
(Embrapa Agropecuaria Oeste. Documentos, 7; Embrapa Soja. Documentos, 134). |
Idioma: |
Português |
Conteúdo: |
Os Convenios da Embrapa com Epamig e produtores de soja de MG, com a Agrosem em Goias e com a Fundacao Cerrados sediada em Brasilia, criaram uma parceria tecnica desde 1997, visando tornar mais eficientes e amplos os testes de genotipos dos ensaios finais para lancamento e indicacao de cultivares de soja. Atualmente os ensaios em rede, resultantes dessa parceria, apresentam os seguuintes numeros de pontos de testes na regiao dos Cerrados: sete em MG, dois no DF, tres na BA, sete em GO e seis em MT. Nesse periodo de dois anos de parceria foram lancadas doze cultivares e indicadas em media, para quatro a cinco estados da regiao do Cerrado. Pontos de teste deverao ser ampliados nos proximos anos. Foram realizados 93 Dias de Campo para lancamento das cultivares, com um alcance direto de 25 mil pessoas. |
Palavras-Chave: |
Brasil; Brasilia; Goias; Minas Gerais; Sacannas; Soybean. |
Thesagro: |
Cerrado; Glycine Max; Soja. |
Thesaurus NAL: |
Brazil; soybeans. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01736naa a2200301 a 4500 001 1456634 005 2006-08-14 008 1999 bl uuuu u00u1 u #d 100 1 $aSOUZA, P. I. de M. 245 $aParceria tecnica entre os convenios da Embrapa em Minas Gerais, Goias e Embrapa Cerrados. 260 $c1999 300 $ap.177. 490 $a(Embrapa Agropecuaria Oeste. Documentos, 7; Embrapa Soja. Documentos, 134). 520 $aOs Convenios da Embrapa com Epamig e produtores de soja de MG, com a Agrosem em Goias e com a Fundacao Cerrados sediada em Brasilia, criaram uma parceria tecnica desde 1997, visando tornar mais eficientes e amplos os testes de genotipos dos ensaios finais para lancamento e indicacao de cultivares de soja. Atualmente os ensaios em rede, resultantes dessa parceria, apresentam os seguuintes numeros de pontos de testes na regiao dos Cerrados: sete em MG, dois no DF, tres na BA, sete em GO e seis em MT. Nesse periodo de dois anos de parceria foram lancadas doze cultivares e indicadas em media, para quatro a cinco estados da regiao do Cerrado. Pontos de teste deverao ser ampliados nos proximos anos. Foram realizados 93 Dias de Campo para lancamento das cultivares, com um alcance direto de 25 mil pessoas. 650 $aBrazil 650 $asoybeans 650 $aCerrado 650 $aGlycine Max 650 $aSoja 653 $aBrasil 653 $aBrasilia 653 $aGoias 653 $aMinas Gerais 653 $aSacannas 653 $aSoybean 700 1 $aARANTES, N. E. 700 1 $aFARIA, L. C. de 773 $tIn: REUNIAO DE PESQUISA DE SOJA DA REGIAO CENTRAL DO BRASIL, 21., 1999, Dourados. Resumos... Dourados: Embrapa Agropecuaria Oeste / Londrina: Embrapa Soja, 1999.
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