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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Meio-Norte. |
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Data corrente: |
10/05/2022 |
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Data da última atualização: |
11/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SALGADO, F. F.; VIEIRA, L. R.; SILVA, V. N. B.; LEAO, A. P.; GRYNBERG, P.; COSTA, M. M. do C.; TOGAWA, R. C.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. |
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Afiliação: |
FERNANDA FERREIRA SALGADO, Universidade Federal de Lavras - UFLA; LETÍCIA RIOS VIEIRA, Universidade Federal de Lavras - UFLA; VIVIANNY NAYSE BELO SILVA, Universidade Federal de Lavras - UFLA; ANDRE PEREIRA LEAO, CNPAE; PRISCILA GRYNBERG, Cenargen; MARCOS MOTA DO CARMO COSTA, Cenargen; ROBERTO COITI TOGAWA, Cenargen; CARLOS ANTONIO FERREIRA DE SOUSA, CPAMN; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE. |
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Título: |
Expression analysis of miRNAs and their putative target genes confirm a preponderant role of transcription factors in the early response of oil palm plants to salinity stress. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
BMC Plant Biology, v. 21, n. 518, 2021. |
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Idioma: |
Inglês |
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Conteúdo: |
Several mechanisms regulating gene expression contribute to restore and reestablish cellular homeostasis so that plants can adapt and survive in adverse situations. MicroRNAs (miRNAs) play roles important in the transcriptional and post-transcriptional regulation of gene expression, emerging as a regulatory molecule key in the responses to plant stress, such as cold, heat, drought, and salt. This work is a comprehensive and large-scale miRNA analysis performed to characterize the miRNA population present in oil palm (Elaeis guineensis Jacq.) exposed to a high level of salt stress, to identify miRNA-putative target genes in the oil palm genome, and to perform an in silico comparison of the expression profile of the miRNAs and their putative target genes. Several mechanisms regulating gene expression contribute to restore and reestablish cellular homeostasis so that plants can adapt and survive in adverse situations. MicroRNAs (miRNAs) play roles important in the transcriptional and post-transcriptional regulation of gene expression, emerging as a regulatory molecule key in the responses to plant stress, such as cold, heat, drought, and salt. This work is a comprehensive and large-scale miRNA analysis performed to characterize the miRNA population present in oil palm (Elaeis guineensis Jacq.) exposed to a high level of salt stress, to identify miRNA-putative target genes in the oil palm genome, and to perform an in silico comparison of the expression profile of the miRNAs and their putative target genes. Our findings provide new insights into the early response of young oil palm plants to salinity stress and confirm an expected preponderant role of transcription factors - such as NF-YA3, HOX32, and GRF1 - in this response. Besides, it points out potential salt-responsive miRNAs and miRNA-putative target genes that one can utilize to develop oil palm plants tolerant to salinity stress. MenosSeveral mechanisms regulating gene expression contribute to restore and reestablish cellular homeostasis so that plants can adapt and survive in adverse situations. MicroRNAs (miRNAs) play roles important in the transcriptional and post-transcriptional regulation of gene expression, emerging as a regulatory molecule key in the responses to plant stress, such as cold, heat, drought, and salt. This work is a comprehensive and large-scale miRNA analysis performed to characterize the miRNA population present in oil palm (Elaeis guineensis Jacq.) exposed to a high level of salt stress, to identify miRNA-putative target genes in the oil palm genome, and to perform an in silico comparison of the expression profile of the miRNAs and their putative target genes. Several mechanisms regulating gene expression contribute to restore and reestablish cellular homeostasis so that plants can adapt and survive in adverse situations. MicroRNAs (miRNAs) play roles important in the transcriptional and post-transcriptional regulation of gene expression, emerging as a regulatory molecule key in the responses to plant stress, such as cold, heat, drought, and salt. This work is a comprehensive and large-scale miRNA analysis performed to characterize the miRNA population present in oil palm (Elaeis guineensis Jacq.) exposed to a high level of salt stress, to identify miRNA-putative target genes in the oil palm genome, and to perform an in silico comparison of the expression profile of the miRNAs and ... Mostrar Tudo |
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Palavras-Chave: |
Estresse abiótico; Fator de transcrição; RNA não-codificante; Transcription factor; Transcriptoma. |
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Thesagro: |
Elaeis Guineensis; RNA. |
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Thesaurus Nal: |
Abiotic stress; Non-coding RNA; Transcriptome. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1142816/1/Salgado-et-al-2021.pdf
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Marc: |
LEADER 02956naa a2200337 a 4500
001 2142816
005 2022-05-11
008 2021 bl uuuu u00u1 u #d
100 1 $aSALGADO, F. F.
245 $aExpression analysis of miRNAs and their putative target genes confirm a preponderant role of transcription factors in the early response of oil palm plants to salinity stress.$h[electronic resource]
260 $c2021
520 $aSeveral mechanisms regulating gene expression contribute to restore and reestablish cellular homeostasis so that plants can adapt and survive in adverse situations. MicroRNAs (miRNAs) play roles important in the transcriptional and post-transcriptional regulation of gene expression, emerging as a regulatory molecule key in the responses to plant stress, such as cold, heat, drought, and salt. This work is a comprehensive and large-scale miRNA analysis performed to characterize the miRNA population present in oil palm (Elaeis guineensis Jacq.) exposed to a high level of salt stress, to identify miRNA-putative target genes in the oil palm genome, and to perform an in silico comparison of the expression profile of the miRNAs and their putative target genes. Several mechanisms regulating gene expression contribute to restore and reestablish cellular homeostasis so that plants can adapt and survive in adverse situations. MicroRNAs (miRNAs) play roles important in the transcriptional and post-transcriptional regulation of gene expression, emerging as a regulatory molecule key in the responses to plant stress, such as cold, heat, drought, and salt. This work is a comprehensive and large-scale miRNA analysis performed to characterize the miRNA population present in oil palm (Elaeis guineensis Jacq.) exposed to a high level of salt stress, to identify miRNA-putative target genes in the oil palm genome, and to perform an in silico comparison of the expression profile of the miRNAs and their putative target genes. Our findings provide new insights into the early response of young oil palm plants to salinity stress and confirm an expected preponderant role of transcription factors - such as NF-YA3, HOX32, and GRF1 - in this response. Besides, it points out potential salt-responsive miRNAs and miRNA-putative target genes that one can utilize to develop oil palm plants tolerant to salinity stress.
650 $aAbiotic stress
650 $aNon-coding RNA
650 $aTranscriptome
650 $aElaeis Guineensis
650 $aRNA
653 $aEstresse abiótico
653 $aFator de transcrição
653 $aRNA não-codificante
653 $aTranscription factor
653 $aTranscriptoma
700 1 $aVIEIRA, L. R.
700 1 $aSILVA, V. N. B.
700 1 $aLEAO, A. P.
700 1 $aGRYNBERG, P.
700 1 $aCOSTA, M. M. do C.
700 1 $aTOGAWA, R. C.
700 1 $aSOUSA, C. A. F. de
700 1 $aSOUZA JUNIOR, M. T.
773 $tBMC Plant Biology$gv. 21, n. 518, 2021.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
09/09/2021 |
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Data da última atualização: |
10/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
SOUZA-FABJAN, J. M. G.; BATISTA, R. I. T. P.; MELO, L. M.; OLIVEIRA, M. A.; CHAVES, M. S.; FONSECA, J. F. da; FREITAS, V. J. F. |
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Afiliação: |
JOANNA M. G. SOUZA-FABJAN, Universidade Federal Fluminense (UFF) -Niterói, RJ, Brazil.; RIBRIO I. T. P. BATISTA, Universidade Federal Fluminense (UFF) -Niterói, RJ, Brazil.; LUCIANA M. MELO, Molecular Genetics Research Unit, University Center Fametro - Fortaleza, CE, Brazil.; MARCOS A. L. OLIVEIRA, Universidade Federal Rural de Pernambuco (UFRPE) - Recife, PE, Brazil.; MAIANA S. CHAVES; JEFERSON FERREIRA DA FONSECA, CNPC; VICENTE J. F. FREITAS, State University of Ceará (UFC) - Fortaleza, CE, Brazil. |
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Título: |
Transcervical versus laparotomy embryo recovery: what strategy is best for embryo bank formation in the Caninde goat conservation program?. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Biopreservation and Biobanking, v. 20, n. 2, p. 204-207, Sept. 2021. |
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DOI: |
http://doi.org/10.1089/bio.2021.0041 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: By allowing for the creation of embryo banks, reproductive biotechnologies play an essential role in the preservation of endangered goat breeds' genetic diversity. This study focused on comparing both available embryo collection methods [laparotomy (LAP) vs. nonsurgical embryo recovery (NSER)] in Canindé goats to create an embryo bank for later use in a breed conservation program. Twelve females were superovulated and subjected to either the LAP or NSER technique for embryo recovery. The recovery rate was similar (p>0.05) between NSER (86.8% ± 5.6%) and LAP (92.8% ± 4.0%). Moreover, there were no differences (p > 0.05) in the number of structures recovered, the viable embryos, and the freezable embryos per goat, respectively, for NSER (11.7 ± 1.3, 11.2 ± 1.5, and 10.2 ± 1.1) and LAP (10.3 ± 1.0, 8.7 ± 0.7, and 8.0 ± 0.8). Overall, 132 structures were collected out of 151 ovulations ( 12.6 ± 1.2 corpora lutea per goat). Finally, the procedure duration time was also similar (p > 0.05) for NSER versus LAP, respectively: 32.3 ± 3.3 versus 30.8 ± 3.9 minutes. In conclusion, the NSER method results proved to be similar to the LAP technique in small-sized Canindé goats. It was noticeable, however, that the NSER technique is simpler and provides the possibility for successive procedures with few health risks and sequels for females. This study may hopefully boost in vivo embryo production programs in the Canindé breed, facilitating the formation of embryo banks and so assuring the availability of genetic diversity before any decline becomes irreversible. MenosAbstract: By allowing for the creation of embryo banks, reproductive biotechnologies play an essential role in the preservation of endangered goat breeds' genetic diversity. This study focused on comparing both available embryo collection methods [laparotomy (LAP) vs. nonsurgical embryo recovery (NSER)] in Canindé goats to create an embryo bank for later use in a breed conservation program. Twelve females were superovulated and subjected to either the LAP or NSER technique for embryo recovery. The recovery rate was similar (p>0.05) between NSER (86.8% ± 5.6%) and LAP (92.8% ± 4.0%). Moreover, there were no differences (p > 0.05) in the number of structures recovered, the viable embryos, and the freezable embryos per goat, respectively, for NSER (11.7 ± 1.3, 11.2 ± 1.5, and 10.2 ± 1.1) and LAP (10.3 ± 1.0, 8.7 ± 0.7, and 8.0 ± 0.8). Overall, 132 structures were collected out of 151 ovulations ( 12.6 ± 1.2 corpora lutea per goat). Finally, the procedure duration time was also similar (p > 0.05) for NSER versus LAP, respectively: 32.3 ± 3.3 versus 30.8 ± 3.9 minutes. In conclusion, the NSER method results proved to be similar to the LAP technique in small-sized Canindé goats. It was noticeable, however, that the NSER technique is simpler and provides the possibility for successive procedures with few health risks and sequels for females. This study may hopefully boost in vivo embryo production programs in the Canindé breed, facilitating the formation of embryo banks and so assu... Mostrar Tudo |
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Palavras-Chave: |
Endangered breed; Germplasm bank; Nonsurgical embryo recovery; NSER. |
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Thesaurus NAL: |
Brazil; Caninde (goat breed); Conservation banking; Conservation programs; Goats; Laparotomy; Reproduction; Species diversity. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02694naa a2200349 a 4500
001 2134141
005 2022-05-10
008 2021 bl uuuu u00u1 u #d
024 7 $ahttp://doi.org/10.1089/bio.2021.0041$2DOI
100 1 $aSOUZA-FABJAN, J. M. G.
245 $aTranscervical versus laparotomy embryo recovery$bwhat strategy is best for embryo bank formation in the Caninde goat conservation program?.$h[electronic resource]
260 $c2021
520 $aAbstract: By allowing for the creation of embryo banks, reproductive biotechnologies play an essential role in the preservation of endangered goat breeds' genetic diversity. This study focused on comparing both available embryo collection methods [laparotomy (LAP) vs. nonsurgical embryo recovery (NSER)] in Canindé goats to create an embryo bank for later use in a breed conservation program. Twelve females were superovulated and subjected to either the LAP or NSER technique for embryo recovery. The recovery rate was similar (p>0.05) between NSER (86.8% ± 5.6%) and LAP (92.8% ± 4.0%). Moreover, there were no differences (p > 0.05) in the number of structures recovered, the viable embryos, and the freezable embryos per goat, respectively, for NSER (11.7 ± 1.3, 11.2 ± 1.5, and 10.2 ± 1.1) and LAP (10.3 ± 1.0, 8.7 ± 0.7, and 8.0 ± 0.8). Overall, 132 structures were collected out of 151 ovulations ( 12.6 ± 1.2 corpora lutea per goat). Finally, the procedure duration time was also similar (p > 0.05) for NSER versus LAP, respectively: 32.3 ± 3.3 versus 30.8 ± 3.9 minutes. In conclusion, the NSER method results proved to be similar to the LAP technique in small-sized Canindé goats. It was noticeable, however, that the NSER technique is simpler and provides the possibility for successive procedures with few health risks and sequels for females. This study may hopefully boost in vivo embryo production programs in the Canindé breed, facilitating the formation of embryo banks and so assuring the availability of genetic diversity before any decline becomes irreversible.
650 $aBrazil
650 $aCaninde (goat breed)
650 $aConservation banking
650 $aConservation programs
650 $aGoats
650 $aLaparotomy
650 $aReproduction
650 $aSpecies diversity
653 $aEndangered breed
653 $aGermplasm bank
653 $aNonsurgical embryo recovery
653 $aNSER
700 1 $aBATISTA, R. I. T. P.
700 1 $aMELO, L. M.
700 1 $aOLIVEIRA, M. A.
700 1 $aCHAVES, M. S.
700 1 $aFONSECA, J. F. da
700 1 $aFREITAS, V. J. F.
773 $tBiopreservation and Biobanking$gv. 20, n. 2, p. 204-207, Sept. 2021.
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