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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
22/03/2022 |
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Data da última atualização: |
22/03/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FALCÃO, M. V. D.; LAROUCAU, K.; VORIMORE, F.; DESHAYES, T.; SANTANA, V. L. A.; SILVA, K. P. C.; NASCIMENTO, S. A. DO; CASTRO, R. S. DE; ARAUJO, F. R.; MOTA, R. A. |
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Afiliação: |
MARCUS V. D. FALCÃO, Universidade Federal Rural de Pernambuco, Departamento de Medicina Veterinária, Recife, Pernambuco, Brasil.; KARINE LAROUCAU, Unidade de Zoonoses Bacterianas, Agência Francesa de Saúde e Segurança Alimentar, Ambiental e Ocupacional (ANSES), Maisons-Alfort, França.; FABIEN VORIMORE, Unidade de Zoonoses Bacterianas, Agência Francesa de Saúde e Segurança Alimentar, Ambiental e Ocupacional (ANSES), Maisons-Alfort, França.; THOMAS DESHAYES, Unidade de Zoonoses Bacterianas, Agência Francesa de Saúde e Segurança Alimentar, Ambiental e Ocupacional (ANSES), Maisons-Alfort, França.; VANIA L. A. SANTANA, Ministério da Agricultura, Pecuária e Abastecimento, Recife, Pernambuco, Brasil.; KARLA P. C. SILVA, Universidade Federal de Alagoas, Departamento de Medicina Veterinária, Maceió, Alagoas, Brasil.; SERGIO A. DO NASCIMENTO, Universidade Federal Rural de Pernambuco, Departamento de Medicina Veterinária, Recife, Pernambuco, Brasil.; ROBERTO S. DE CASTRO, Universidade Federal Rural de Pernambuco, Departamento de Medicina Veterinária, Recife, Pernambuco, Brasil.; FLABIO RIBEIRO DE ARAUJO, CNPGC; RINALDO A. MOTA, Universidade Federal Rural de Pernambuco, Departamento de Medicina Veterinária, Recife, Pernambuco, Brasil. |
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Título: |
Molecular characterization of Burkholderia mallei strains isolated from horses in Brazil (2014-2017). |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Infection, Genetics and Evolution, v. 99, 2022. |
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Idioma: |
Inglês |
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Conteúdo: |
Glanders is an infectious zoonosis caused by Burkholderia (B.) mallei that mainly affects equids. The objective of this work was to provide additional knowledge on the diversity of the strains circulating in Brazil. Six Burkholderia mallei isolates obtained during necropsies of glanderous horses between 2014 and 2017 in two different states (Pernambuco and Alagoas) were analyzed by polymerase chain reaction?high-resolution melting (PCRHRM). While four strains (9902 RSC, BM_campo 1, BM_campo 3 and UFAL2) clustered in the L3B2 branch, which already includes the Brazilian 16-2438_BM#8 strain, two strains (BM_campo 2.1 and BM_campo 2.2) clustered within the L3B3sB3 branch, which mostly includes older isolates, from Europe and the Middle East. Whole genome sequencing of two of these strains (UFAL2 and BM_campo 2.1), belonging to different branches, confirmed the HRM typing results and refined the links between the strains, including the description of the L3B3Sb3Gp1SbGp1 genotype, never reported so far for contemporary strains. These results suggest different glanders introduction events in Brazil, including a potential link with strains of European origin, related to colonization or trade. |
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Palavras-Chave: |
PCR-HRM; WGS. |
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Thesaurus Nal: |
Burkholderia mallei; Genotyping; Horses. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/232845/1/2022-Genoma-Burkholkderia-mallei.pdf
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Marc: |
LEADER 02014naa a2200289 a 4500
001 2141175
005 2022-03-22
008 2022 bl uuuu u00u1 u #d
100 1 $aFALCÃO, M. V. D.
245 $aMolecular characterization of Burkholderia mallei strains isolated from horses in Brazil (2014-2017).$h[electronic resource]
260 $c2022
520 $aGlanders is an infectious zoonosis caused by Burkholderia (B.) mallei that mainly affects equids. The objective of this work was to provide additional knowledge on the diversity of the strains circulating in Brazil. Six Burkholderia mallei isolates obtained during necropsies of glanderous horses between 2014 and 2017 in two different states (Pernambuco and Alagoas) were analyzed by polymerase chain reaction?high-resolution melting (PCRHRM). While four strains (9902 RSC, BM_campo 1, BM_campo 3 and UFAL2) clustered in the L3B2 branch, which already includes the Brazilian 16-2438_BM#8 strain, two strains (BM_campo 2.1 and BM_campo 2.2) clustered within the L3B3sB3 branch, which mostly includes older isolates, from Europe and the Middle East. Whole genome sequencing of two of these strains (UFAL2 and BM_campo 2.1), belonging to different branches, confirmed the HRM typing results and refined the links between the strains, including the description of the L3B3Sb3Gp1SbGp1 genotype, never reported so far for contemporary strains. These results suggest different glanders introduction events in Brazil, including a potential link with strains of European origin, related to colonization or trade.
650 $aBurkholderia mallei
650 $aGenotyping
650 $aHorses
653 $aPCR-HRM
653 $aWGS
700 1 $aLAROUCAU, K.
700 1 $aVORIMORE, F.
700 1 $aDESHAYES, T.
700 1 $aSANTANA, V. L. A.
700 1 $aSILVA, K. P. C.
700 1 $aNASCIMENTO, S. A. DO
700 1 $aCASTRO, R. S. DE
700 1 $aARAUJO, F. R.
700 1 $aMOTA, R. A.
773 $tInfection, Genetics and Evolution$gv. 99, 2022.
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Embrapa Gado de Corte (CNPGC) |
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| 1. |  | FALCÃO, M. V. D.; LAROUCAU, K.; VORIMORE, F.; DESHAYES, T.; SANTANA, V. L. A.; SILVA, K. P. C.; NASCIMENTO, S. A. DO; CASTRO, R. S. DE; ARAUJO, F. R.; MOTA, R. A. Molecular characterization of Burkholderia mallei strains isolated from horses in Brazil (2014-2017). Infection, Genetics and Evolution, v. 99, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Corte. |
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