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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
26/01/2022 |
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Data da última atualização: |
26/01/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ALMEIDA, D. P. de; CASTRO, I. S. L.; MENDES, T. A. de O.; ALVES, D. R.; BARKA, G. D.; BARREIROS, P. R. R. M.; ZAMBOLIM, L.; SAKIYAMA, N. S.; CAIXETA, E. T. |
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Afiliação: |
DÊNIA PIRES DE ALMEIDA, UFV; ISABEL SAMILA LIMA CASTRO, UFV; TIAGO ANTÔNIO DE OLIVEIRA MENDES, UFV; DANÚBIA RODRIGUES ALVES, UFV; GELETA DUGASSA BARKA, UFV; PEDRO RICARDO ROSSI MARQUES BARREIROS, UFV; LAÉRCIO ZAMBOLIM, UFV; NEY SUSSUMU SAKIYAMA, UFV; EVELINE TEIXEIRA CAIXETA MOURA, CNPCa. |
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Título: |
Receptor-Like Kinase (RLK) as a candidate gene conferring resistance to Hemileia vastatrix in coffee. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Scientia Agricola, v.78, n.6, e20200023, 2021. |
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DOI: |
https://doi.org/10.1590/1678-992X-2020-0023 |
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Idioma: |
Inglês |
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Conteúdo: |
The biotrophic fungus Hemileia vastatrix causes coffee leaf rust (CLR), one of the most devastating diseases in Coffea arabica . Coffee, like other plants, has developed effective mechanisms to recognize and respond to infections caused by pathogens. Plant resistance gene analogs (RGAs) have been identified in certain plants as candidates for resistance ( R ) genes or membrane receptors that activate the R genes. The RGAs identified in different plants possess conserved domains that play specific roles in the fight against pathogens. Despite the importance of RGAs, in coffee plants these genes and other molecular mechanisms of disease resistance are still unknown. This study aimed to sequence and characterize candidate genes from coffee plants with the potential for involvement in resistance to H. vastatrix . Sequencing was performed based on a library of bacterial artificial chromosomes (BAC) of the coffee clone ?Híbrido de Timor? (HdT) CIFC 832/2 and screened using a functional marker. Two RGAs, HdT_LRR_RLK1 and HdT_LRR_RLK2, containing the motif of leucine-rich repeat-like kinase (LRR-RLK) were identified. Based on the presence or absence of the HdT_LRR_RLK2 RGA in a number of differential coffee clones containing different combinations of the rust resistance gene, these RGAs did not correspond to any resistance gene already characterized (SH1-9). These genes were also analyzed using qPCR and demonstrated a major expression peak at 24 h after inoculation in both the compatible and incompatible interactions between coffee and H. vastatrix . These results are valuable information for breeding programs aimed at developing CLR-resistant cultivars, in addition to enabling a better understanding of the interactions between coffee and H. vastatrix. MenosThe biotrophic fungus Hemileia vastatrix causes coffee leaf rust (CLR), one of the most devastating diseases in Coffea arabica . Coffee, like other plants, has developed effective mechanisms to recognize and respond to infections caused by pathogens. Plant resistance gene analogs (RGAs) have been identified in certain plants as candidates for resistance ( R ) genes or membrane receptors that activate the R genes. The RGAs identified in different plants possess conserved domains that play specific roles in the fight against pathogens. Despite the importance of RGAs, in coffee plants these genes and other molecular mechanisms of disease resistance are still unknown. This study aimed to sequence and characterize candidate genes from coffee plants with the potential for involvement in resistance to H. vastatrix . Sequencing was performed based on a library of bacterial artificial chromosomes (BAC) of the coffee clone ?Híbrido de Timor? (HdT) CIFC 832/2 and screened using a functional marker. Two RGAs, HdT_LRR_RLK1 and HdT_LRR_RLK2, containing the motif of leucine-rich repeat-like kinase (LRR-RLK) were identified. Based on the presence or absence of the HdT_LRR_RLK2 RGA in a number of differential coffee clones containing different combinations of the rust resistance gene, these RGAs did not correspond to any resistance gene already characterized (SH1-9). These genes were also analyzed using qPCR and demonstrated a major expression peak at 24 h after inoculation in both the compa... Mostrar Tudo |
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Palavras-Chave: |
Receptor-Like Kinase. |
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Thesagro: |
Hemileia Vastatrix. |
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Thesaurus Nal: |
Clones; Coffea arabica var. arabica; Hemileia; Leaf rust; Plant breeding; Plant diseases and disorders. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/230506/1/receptor-like-kinase.pdf
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Marc: |
LEADER 02762naa a2200325 a 4500
001 2139319
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008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/1678-992X-2020-0023$2DOI
100 1 $aALMEIDA, D. P. de
245 $aReceptor-Like Kinase (RLK) as a candidate gene conferring resistance to Hemileia vastatrix in coffee.$h[electronic resource]
260 $c2021
520 $aThe biotrophic fungus Hemileia vastatrix causes coffee leaf rust (CLR), one of the most devastating diseases in Coffea arabica . Coffee, like other plants, has developed effective mechanisms to recognize and respond to infections caused by pathogens. Plant resistance gene analogs (RGAs) have been identified in certain plants as candidates for resistance ( R ) genes or membrane receptors that activate the R genes. The RGAs identified in different plants possess conserved domains that play specific roles in the fight against pathogens. Despite the importance of RGAs, in coffee plants these genes and other molecular mechanisms of disease resistance are still unknown. This study aimed to sequence and characterize candidate genes from coffee plants with the potential for involvement in resistance to H. vastatrix . Sequencing was performed based on a library of bacterial artificial chromosomes (BAC) of the coffee clone ?Híbrido de Timor? (HdT) CIFC 832/2 and screened using a functional marker. Two RGAs, HdT_LRR_RLK1 and HdT_LRR_RLK2, containing the motif of leucine-rich repeat-like kinase (LRR-RLK) were identified. Based on the presence or absence of the HdT_LRR_RLK2 RGA in a number of differential coffee clones containing different combinations of the rust resistance gene, these RGAs did not correspond to any resistance gene already characterized (SH1-9). These genes were also analyzed using qPCR and demonstrated a major expression peak at 24 h after inoculation in both the compatible and incompatible interactions between coffee and H. vastatrix . These results are valuable information for breeding programs aimed at developing CLR-resistant cultivars, in addition to enabling a better understanding of the interactions between coffee and H. vastatrix.
650 $aClones
650 $aCoffea arabica var. arabica
650 $aHemileia
650 $aLeaf rust
650 $aPlant breeding
650 $aPlant diseases and disorders
650 $aHemileia Vastatrix
653 $aReceptor-Like Kinase
700 1 $aCASTRO, I. S. L.
700 1 $aMENDES, T. A. de O.
700 1 $aALVES, D. R.
700 1 $aBARKA, G. D.
700 1 $aBARREIROS, P. R. R. M.
700 1 $aZAMBOLIM, L.
700 1 $aSAKIYAMA, N. S.
700 1 $aCAIXETA, E. T.
773 $tScientia Agricola$gv.78, n.6, e20200023, 2021.
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Registro original: |
Embrapa Café (CNPCa) |
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| 1. |  | ALMEIDA, D. P. de; CASTRO, I. S. L.; MENDES, T. A. de O.; ALVES, D. R.; BARKA, G. D.; BARREIROS, P. R. R. M.; ZAMBOLIM, L.; SAKIYAMA, N. S.; CAIXETA, E. T. Receptor-Like Kinase (RLK) as a candidate gene conferring resistance to Hemileia vastatrix in coffee. Scientia Agricola, v.78, n.6, e20200023, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Café. |
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