| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Rondônia. |
|
Data corrente: |
13/12/2021 |
|
Data da última atualização: |
14/12/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SOUZA, C. A. de; ROCHA, R. B.; SANTOS, M. R. A. dos; LOPES, T. A.; TEIXEIRA, A. L.; ESPINDULA, M. C. |
|
Afiliação: |
CAROLINA AUGUSTO DE SOUZA, Universidade Federal de Rondônia (UNIR); RODRIGO BARROS ROCHA, CPAF-RO; MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO; TATIANE ALMEIDA LOPES; ALEXSANDRO LARA TEIXEIRA, CPAF-RO; MARCELO CURITIBA ESPINDULA, CPAF-RO. |
|
Título: |
In vitro pollination and fluorescence microscopy for characterization of gametophytic selfincompatibility of Coffea canephora Pierre ex A. Froehner. |
|
Ano de publicação: |
2021 |
|
Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, v. 21, n. 4, e37692142, 2021. |
|
ISSN: |
1518-7853 (impresso) / 1984-7033 (online) |
|
DOI: |
http://dx.doi.org/10.1590/1984- 70332021v21n4a51 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The objectives of this study were to optimize in vitro pollination and fluorescence microscopy procedures to characterize the gametophytic selfincompatibility of Coffea canephora genotypes. For that purpose, 115 in vitro pollinations were carried out among 30 parent plants of the Conilon (17) and Robusta (13) botanical varieties. Tester plants of compatibility groups I, II, and III were used as pollen grain donors. On the day of anthesis, pollen grains of the donor genotypes were transferred in vitro to one of the sides of the bifid stigma of each receptor flower. After 30 hours, the development of the pollen tubes was evaluated in fluorescence microscopy. Eleven genotypes (37%) were classified in compatibility Group I, thirteen (43%) in Group II, and six (20%) in Group III. In vitro pollination allowed reduction in the time necessary to diagnose compatibility, without exhibiting contamination caused by the presence of pollen grains of non-donor plants. |
|
Palavras-Chave: |
Café Conilon; In vitro pollination; Microscópio Fluorescente. |
|
Thesagro: |
Café Robusta; Coffea Canephora; Cultura In Vitro; Genótipo; Polinização; Reprodução Vegetal; Variação Genética. |
|
Thesaurus Nal: |
Fluorescence microscopy; Genetic variation; Plant reproduction. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/229056/1/cpafro-18641.pdf
|
|
Marc: |
LEADER 02166naa a2200361 a 4500
001 2137706
005 2021-12-14
008 2021 bl uuuu u00u1 u #d
022 $a1518-7853 (impresso) / 1984-7033 (online)
024 7 $ahttp://dx.doi.org/10.1590/1984- 70332021v21n4a51$2DOI
100 1 $aSOUZA, C. A. de
245 $aIn vitro pollination and fluorescence microscopy for characterization of gametophytic selfincompatibility of Coffea canephora Pierre ex A. Froehner.$h[electronic resource]
260 $c2021
520 $aThe objectives of this study were to optimize in vitro pollination and fluorescence microscopy procedures to characterize the gametophytic selfincompatibility of Coffea canephora genotypes. For that purpose, 115 in vitro pollinations were carried out among 30 parent plants of the Conilon (17) and Robusta (13) botanical varieties. Tester plants of compatibility groups I, II, and III were used as pollen grain donors. On the day of anthesis, pollen grains of the donor genotypes were transferred in vitro to one of the sides of the bifid stigma of each receptor flower. After 30 hours, the development of the pollen tubes was evaluated in fluorescence microscopy. Eleven genotypes (37%) were classified in compatibility Group I, thirteen (43%) in Group II, and six (20%) in Group III. In vitro pollination allowed reduction in the time necessary to diagnose compatibility, without exhibiting contamination caused by the presence of pollen grains of non-donor plants.
650 $aFluorescence microscopy
650 $aGenetic variation
650 $aPlant reproduction
650 $aCafé Robusta
650 $aCoffea Canephora
650 $aCultura In Vitro
650 $aGenótipo
650 $aPolinização
650 $aReprodução Vegetal
650 $aVariação Genética
653 $aCafé Conilon
653 $aIn vitro pollination
653 $aMicroscópio Fluorescente
700 1 $aROCHA, R. B.
700 1 $aSANTOS, M. R. A. dos
700 1 $aLOPES, T. A.
700 1 $aTEIXEIRA, A. L.
700 1 $aESPINDULA, M. C.
773 $tCrop Breeding and Applied Biotechnology$gv. 21, n. 4, e37692142, 2021.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Rondônia (CPAF-RO) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 1 | |
| 1. |  | SOUZA, C. A. de; ROCHA, R. B.; SANTOS, M. R. A. dos; LOPES, T. A.; TEIXEIRA, A. L.; ESPINDULA, M. C. In vitro pollination and fluorescence microscopy for characterization of gametophytic selfincompatibility of Coffea canephora Pierre ex A. Froehner. Crop Breeding and Applied Biotechnology, v. 21, n. 4, e37692142, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Rondônia. |
|    |
| Registros recuperados : 1 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
