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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
09/12/2021 |
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Data da última atualização: |
09/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LOPES, P. D.; OKINO, C. H.; FERNANDO, F. S.; PAVANI, C.; MARIGUELA, V. C.; MONTASSIER, M. DE F. S.; MONTASSIER, H. J. |
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Afiliação: |
PRISCILA DINIZ LOPES, UNESP; CINTIA HIROMI OKINO, CPPSE; FILIPE SANTOS FERNANDO, UNESP; CAREN PAVANI, UNESP; VIVIANE CASAGRANDE MARIGUELA, UNESP; MARIA DE FÁTIMA SILVA MONTASSIER, UNESP; HÉLIO JOSÉ MONTASSIER, UNESP. |
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Título: |
Comparative evaluation of immune responses and protection of chitosan nanoparticles and oil-emulsion adjuvants in avian coronavirus inactivated vaccines in chickens. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Vaccines, v.9, n.12, 1457, 2021. |
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ISSN: |
2076-393X |
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DOI: |
https://doi.org/10.3390/vaccines9121457 |
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Idioma: |
Inglês |
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Conteúdo: |
Efficient vaccines are the main strategy to control the avian coronavirus (AvCoV), although several drawbacks related to traditional attenuated and inactivated vaccines have been reported. These counterpoints highlight the importance of developing new alternative vaccines against AvCoV, especially those able to induce long-lasting immune responses. This study evaluated and compared two inactivated vaccines formulated with AvCoV BR-I variants, one composed of chitosan nanoparticles (AvCoV-CS) and the second by Montanide oily adjuvant (AvCoV-O). Both developed vaccines were administered in a single dose or associated with the traditional Mass attenuated vaccine. The AvCoV-CS vaccine administered alone or associated with the Mass vaccine was able to induce strong humoral and cell-mediated immune (CMI) responses and complete protection against IBV virulent infection, wherein single administration was characterized by high IgA antibody levels in the mucosa, whereas when associated with the Mass vaccine, the serum IgG antibody was predominantly observed. On the other hand, single administration of the oily vaccine presented poor humoral and CMI responses and consequently incomplete protection against virulent challenge, but when associated with the Mass vaccine, immune responses were developed, and complete protection against infection was observed. Both of our experimental vaccines were able to induce full protection against virulent IBV challenge. A single dose of AvCoV-CS vaccine was sufficient to achieve complete protection, while AvCoV-O required a previous priming by a Mass strain to complete the protection. MenosEfficient vaccines are the main strategy to control the avian coronavirus (AvCoV), although several drawbacks related to traditional attenuated and inactivated vaccines have been reported. These counterpoints highlight the importance of developing new alternative vaccines against AvCoV, especially those able to induce long-lasting immune responses. This study evaluated and compared two inactivated vaccines formulated with AvCoV BR-I variants, one composed of chitosan nanoparticles (AvCoV-CS) and the second by Montanide oily adjuvant (AvCoV-O). Both developed vaccines were administered in a single dose or associated with the traditional Mass attenuated vaccine. The AvCoV-CS vaccine administered alone or associated with the Mass vaccine was able to induce strong humoral and cell-mediated immune (CMI) responses and complete protection against IBV virulent infection, wherein single administration was characterized by high IgA antibody levels in the mucosa, whereas when associated with the Mass vaccine, the serum IgG antibody was predominantly observed. On the other hand, single administration of the oily vaccine presented poor humoral and CMI responses and consequently incomplete protection against virulent challenge, but when associated with the Mass vaccine, immune responses were developed, and complete protection against infection was observed. Both of our experimental vaccines were able to induce full protection against virulent IBV challenge. A single dose of AvCoV-CS vacci... Mostrar Tudo |
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Palavras-Chave: |
Avian infectious bronchitis; Cellular immune response; Delivery carrier adjuvant; Mucosal immunization; Oil adjuvant. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/228815/1/ComparativeEvaluationImmune.pdf
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Marc: |
LEADER 02553naa a2200277 a 4500
001 2137397
005 2021-12-09
008 2021 bl uuuu u00u1 u #d
022 $a2076-393X
024 7 $ahttps://doi.org/10.3390/vaccines9121457$2DOI
100 1 $aLOPES, P. D.
245 $aComparative evaluation of immune responses and protection of chitosan nanoparticles and oil-emulsion adjuvants in avian coronavirus inactivated vaccines in chickens.$h[electronic resource]
260 $c2021
520 $aEfficient vaccines are the main strategy to control the avian coronavirus (AvCoV), although several drawbacks related to traditional attenuated and inactivated vaccines have been reported. These counterpoints highlight the importance of developing new alternative vaccines against AvCoV, especially those able to induce long-lasting immune responses. This study evaluated and compared two inactivated vaccines formulated with AvCoV BR-I variants, one composed of chitosan nanoparticles (AvCoV-CS) and the second by Montanide oily adjuvant (AvCoV-O). Both developed vaccines were administered in a single dose or associated with the traditional Mass attenuated vaccine. The AvCoV-CS vaccine administered alone or associated with the Mass vaccine was able to induce strong humoral and cell-mediated immune (CMI) responses and complete protection against IBV virulent infection, wherein single administration was characterized by high IgA antibody levels in the mucosa, whereas when associated with the Mass vaccine, the serum IgG antibody was predominantly observed. On the other hand, single administration of the oily vaccine presented poor humoral and CMI responses and consequently incomplete protection against virulent challenge, but when associated with the Mass vaccine, immune responses were developed, and complete protection against infection was observed. Both of our experimental vaccines were able to induce full protection against virulent IBV challenge. A single dose of AvCoV-CS vaccine was sufficient to achieve complete protection, while AvCoV-O required a previous priming by a Mass strain to complete the protection.
653 $aAvian infectious bronchitis
653 $aCellular immune response
653 $aDelivery carrier adjuvant
653 $aMucosal immunization
653 $aOil adjuvant
700 1 $aOKINO, C. H.
700 1 $aFERNANDO, F. S.
700 1 $aPAVANI, C.
700 1 $aMARIGUELA, V. C.
700 1 $aMONTASSIER, M. DE F. S.
700 1 $aMONTASSIER, H. J.
773 $tVaccines$gv.9, n.12, 1457, 2021.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Biblioteca |
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Tipo/Formato |
Classificação |
Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
09/05/2022 |
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Data da última atualização: |
09/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
AMARAL, T. F.; GRAZIA, J. G. V. de; MARTINHAO, L A. G.; DE COL, F.; SIQUEIRA, L. G. B.; VIANA, J. H. M.; HANSEN, P. J. |
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Afiliação: |
THIAGO F. AMARAL, University of Florida; JOAO GABRIEL VIANA DE GRAZIA, FIVX Apoyar Biotech Ltda; LUANY ALVES GALVAO MARTINHAO, FIVX Apoyar Biotech Ltda; FELIPE DE COL, FIVX Apoyar Biotech Ltda; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen; PETER J. HANSEN, University of Florida. |
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Título: |
Actions of CSF2 and DKK1 on bovine embryo development and pregnancy outcomes are affected by composition of embryo culture medium. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Scientific Reports, v. 12, 7503, 2022. |
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DOI: |
https://doi.org/10.1038/s41598-022-11447-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Procedures for in vitro embryo production in cattle have not been optimized. In the current experiment, we utilized a 3 × 3 factorial design to test whether the proportion of embryos becoming blastocysts in culture and the pregnancy rate after embryo transfer are afected by type of serum in the medium [no serum; 3% (v/v) KnockOut Serum Replacement (SR); 3% (v/v) fetal bovine serum (FBS)] and addition of specifc embryokines [vehicle; 10 ng/mL colony stimulating factor 2 (CSF2); 100 ng/mL dickkopf related protein 1 (DKK1)] at day 5 of culture. Embryos were produced using abattoir-derived ovaries and Y-sorted semen from two Angus sires. The percent of putative zygotes and cleaved embryos becoming blastocysts was improved by SR and FBS. Pregnancy rate at day 30 was determined for 1426 Nelore recipients and calving rate for 266 recipients. In the absence of CSF2 or DKK1, pregnancy rates were lower for embryos cultured with SR or FBS. CSF2 and DKK1 reduced pregnancy rate for embryos cultured without serum but had no detrimental efect in the SR or FBS groups. Indeed, CSF2 blocked the negative efect of FBS on pregnancy rate. Data on birth weights were available for 67 bull calves. There were no efects of treatment. The sire used to produce embryos had signifcant and large efects on development to the blastocyst stage, pregnancy rate at day 30, calving rate and pregnancy loss between day 30 and calving. Results indicate that (1) SR and FBS can improve embryonic development in vitro while also compromising competence of embryos to survive after transfer, (2) actions of CSF2 and DKK1 depend upon other characteristics of the embryo production system, and (3) sire can have a large efect on embryonic development before and after transfer. MenosProcedures for in vitro embryo production in cattle have not been optimized. In the current experiment, we utilized a 3 × 3 factorial design to test whether the proportion of embryos becoming blastocysts in culture and the pregnancy rate after embryo transfer are afected by type of serum in the medium [no serum; 3% (v/v) KnockOut Serum Replacement (SR); 3% (v/v) fetal bovine serum (FBS)] and addition of specifc embryokines [vehicle; 10 ng/mL colony stimulating factor 2 (CSF2); 100 ng/mL dickkopf related protein 1 (DKK1)] at day 5 of culture. Embryos were produced using abattoir-derived ovaries and Y-sorted semen from two Angus sires. The percent of putative zygotes and cleaved embryos becoming blastocysts was improved by SR and FBS. Pregnancy rate at day 30 was determined for 1426 Nelore recipients and calving rate for 266 recipients. In the absence of CSF2 or DKK1, pregnancy rates were lower for embryos cultured with SR or FBS. CSF2 and DKK1 reduced pregnancy rate for embryos cultured without serum but had no detrimental efect in the SR or FBS groups. Indeed, CSF2 blocked the negative efect of FBS on pregnancy rate. Data on birth weights were available for 67 bull calves. There were no efects of treatment. The sire used to produce embryos had signifcant and large efects on development to the blastocyst stage, pregnancy rate at day 30, calving rate and pregnancy loss between day 30 and calving. Results indicate that (1) SR and FBS can improve embryonic development in vitro w... Mostrar Tudo |
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Palavras-Chave: |
Fertilização; Produção in vitro. |
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Thesagro: |
Bovino; Embrião; Reprodução Animal. |
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Thesaurus NAL: |
Animal reproduction; Embryo (animal); In vitro fertilization. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1142749/1/Actions-of-CSF2.pdf
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Marc: |
LEADER 02687naa a2200301 a 4500
001 2142749
005 2022-05-09
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1038/s41598-022-11447-7$2DOI
100 1 $aAMARAL, T. F.
245 $aActions of CSF2 and DKK1 on bovine embryo development and pregnancy outcomes are affected by composition of embryo culture medium.$h[electronic resource]
260 $c2022
520 $aProcedures for in vitro embryo production in cattle have not been optimized. In the current experiment, we utilized a 3 × 3 factorial design to test whether the proportion of embryos becoming blastocysts in culture and the pregnancy rate after embryo transfer are afected by type of serum in the medium [no serum; 3% (v/v) KnockOut Serum Replacement (SR); 3% (v/v) fetal bovine serum (FBS)] and addition of specifc embryokines [vehicle; 10 ng/mL colony stimulating factor 2 (CSF2); 100 ng/mL dickkopf related protein 1 (DKK1)] at day 5 of culture. Embryos were produced using abattoir-derived ovaries and Y-sorted semen from two Angus sires. The percent of putative zygotes and cleaved embryos becoming blastocysts was improved by SR and FBS. Pregnancy rate at day 30 was determined for 1426 Nelore recipients and calving rate for 266 recipients. In the absence of CSF2 or DKK1, pregnancy rates were lower for embryos cultured with SR or FBS. CSF2 and DKK1 reduced pregnancy rate for embryos cultured without serum but had no detrimental efect in the SR or FBS groups. Indeed, CSF2 blocked the negative efect of FBS on pregnancy rate. Data on birth weights were available for 67 bull calves. There were no efects of treatment. The sire used to produce embryos had signifcant and large efects on development to the blastocyst stage, pregnancy rate at day 30, calving rate and pregnancy loss between day 30 and calving. Results indicate that (1) SR and FBS can improve embryonic development in vitro while also compromising competence of embryos to survive after transfer, (2) actions of CSF2 and DKK1 depend upon other characteristics of the embryo production system, and (3) sire can have a large efect on embryonic development before and after transfer.
650 $aAnimal reproduction
650 $aEmbryo (animal)
650 $aIn vitro fertilization
650 $aBovino
650 $aEmbrião
650 $aReprodução Animal
653 $aFertilização
653 $aProdução in vitro
700 1 $aGRAZIA, J. G. V. de
700 1 $aMARTINHAO, L A. G.
700 1 $aDE COL, F.
700 1 $aSIQUEIRA, L. G. B.
700 1 $aVIANA, J. H. M.
700 1 $aHANSEN, P. J.
773 $tScientific Reports$gv. 12, 7503, 2022.
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Embrapa Gado de Leite (CNPGL) |
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