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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
17/11/2021 |
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Data da última atualização: |
17/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, J. D. R.; BATISTA, R. I. T. P.; UNGERFELD, R.; TAIRA, A. R.; ESPÍRITO SANTO, C. G. do; SOUZA-FABJAN, J. M. G.; FERNANDES, D. A. de M.; BALARO, M. F. A.; COSENTINO, I. O.; BRAIR, V. L.; PINTO, P. H. N.; CARVALHO, A. B. da S.; FONSECA, J. F. da; BRANDÃO, F. Z. |
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Afiliação: |
JULIANA DANTAS RODRIGUES SANTOS, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; RIBRIO IVAN TAVARES PEREIRA BATISTA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; RODOLFO UNGERFELD, Universidad de la República - Montevideo, Uruguay; AUGUSTO RYONOSUKE TAIRA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; CAROLINE GOMES DO ESPÍRITO SANTO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; JOANNA MARIA GONÇALVES SOUZA-FABJAN, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; DANIEL ANDREWS DE MOURA FERNANDES, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; MÁRIO FELIPE ALVAREZ BALARO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; ISABEL OLIVEIRA COSENTINO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; VIVIANE LOPES BRAIR, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; PEDRO HENRIQUE NICOLAU PINTO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; ANA BEATRIZ DA SILVA CARVALHO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; JEFERSON FERREIRA DA FONSECA, CNPC; FELIPE ZANDONADI BRANDÃO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil. |
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Título: |
Hormonal protocol used for cervical dilation in ewes does not affect morphological embryo quality but reduces recovery rate and temporarily alters gene expression. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Veterinary Record, e1064, 2021. |
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DOI: |
https://doi.org/10.1002/vetr.1064 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Background: Information on the impact of hormonal protocols for cervical dilation on the quality of ovine embryos is scarce. Methods: To compare the quality of embryos after cervical dilation protocol, ewes (n = 64) were allocated into either a treated group (100 ?g estradiol benzoate intravenous and 0.12 mg cloprostenol intramuscularly, 12 hours before embryo collection plus 100 iu oxytocin intravenous 15 minutes before the collection procedure) or a control group (saline). Luteal function was analysed using ultrasonography and P4 measurement. Some collected embryos were frozen/thawed for gene expression, others were cultured in vitro, frozen/thawed for gene expression, and the remaining embryos were fixed for the apoptosis test (TUNEL test). Results: The treatment reduced fluid (p=0.04) and structure (p=0.03) recovery rates, but the morphological quality, development stage, and apoptosis incidence of the embryos were not affected by treatment. The corpora lutea of the control group had greater blood perfusion (p = 0.002) and greater P4 concentrations at 6, 9, and 12 h after the treatment (p < 0.0001). The expression of BAX, BCL2, PRDX1, and HSP90 genes were not affected by the treatment. However, the embryos in the treated group had fewer NANOG and OCT4 transcripts than control embryos (p = 0.008; p = 0.006, respectively). After culture, there was no difference between the groups in any gene. Conclusion: The hormonal protocol for cervical dilation reduced the efficiency of embryo collection. In addition, the treatment induced luteolysis and a transient alteration of embryo gene expression, however there were no detectable changes in embryo morphological quality, development stage, or incidence of apoptosis. MenosAbstract: Background: Information on the impact of hormonal protocols for cervical dilation on the quality of ovine embryos is scarce. Methods: To compare the quality of embryos after cervical dilation protocol, ewes (n = 64) were allocated into either a treated group (100 ?g estradiol benzoate intravenous and 0.12 mg cloprostenol intramuscularly, 12 hours before embryo collection plus 100 iu oxytocin intravenous 15 minutes before the collection procedure) or a control group (saline). Luteal function was analysed using ultrasonography and P4 measurement. Some collected embryos were frozen/thawed for gene expression, others were cultured in vitro, frozen/thawed for gene expression, and the remaining embryos were fixed for the apoptosis test (TUNEL test). Results: The treatment reduced fluid (p=0.04) and structure (p=0.03) recovery rates, but the morphological quality, development stage, and apoptosis incidence of the embryos were not affected by treatment. The corpora lutea of the control group had greater blood perfusion (p = 0.002) and greater P4 concentrations at 6, 9, and 12 h after the treatment (p < 0.0001). The expression of BAX, BCL2, PRDX1, and HSP90 genes were not affected by the treatment. However, the embryos in the treated group had fewer NANOG and OCT4 transcripts than control embryos (p = 0.008; p = 0.006, respectively). After culture, there was no difference between the groups in any gene. Conclusion: The hormonal protocol for cervical dilation reduced the eff... Mostrar Tudo |
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Thesaurus Nal: |
Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02718naa a2200301 a 4500
001 2136205
005 2021-11-17
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1002/vetr.1064$2DOI
100 1 $aSANTOS, J. D. R.
245 $aHormonal protocol used for cervical dilation in ewes does not affect morphological embryo quality but reduces recovery rate and temporarily alters gene expression.$h[electronic resource]
260 $c2021
520 $aAbstract: Background: Information on the impact of hormonal protocols for cervical dilation on the quality of ovine embryos is scarce. Methods: To compare the quality of embryos after cervical dilation protocol, ewes (n = 64) were allocated into either a treated group (100 ?g estradiol benzoate intravenous and 0.12 mg cloprostenol intramuscularly, 12 hours before embryo collection plus 100 iu oxytocin intravenous 15 minutes before the collection procedure) or a control group (saline). Luteal function was analysed using ultrasonography and P4 measurement. Some collected embryos were frozen/thawed for gene expression, others were cultured in vitro, frozen/thawed for gene expression, and the remaining embryos were fixed for the apoptosis test (TUNEL test). Results: The treatment reduced fluid (p=0.04) and structure (p=0.03) recovery rates, but the morphological quality, development stage, and apoptosis incidence of the embryos were not affected by treatment. The corpora lutea of the control group had greater blood perfusion (p = 0.002) and greater P4 concentrations at 6, 9, and 12 h after the treatment (p < 0.0001). The expression of BAX, BCL2, PRDX1, and HSP90 genes were not affected by the treatment. However, the embryos in the treated group had fewer NANOG and OCT4 transcripts than control embryos (p = 0.008; p = 0.006, respectively). After culture, there was no difference between the groups in any gene. Conclusion: The hormonal protocol for cervical dilation reduced the efficiency of embryo collection. In addition, the treatment induced luteolysis and a transient alteration of embryo gene expression, however there were no detectable changes in embryo morphological quality, development stage, or incidence of apoptosis.
650 $aSheep
700 1 $aBATISTA, R. I. T. P.
700 1 $aUNGERFELD, R.
700 1 $aTAIRA, A. R.
700 1 $aESPÍRITO SANTO, C. G. do
700 1 $aSOUZA-FABJAN, J. M. G.
700 1 $aFERNANDES, D. A. de M.
700 1 $aBALARO, M. F. A.
700 1 $aCOSENTINO, I. O.
700 1 $aBRAIR, V. L.
700 1 $aPINTO, P. H. N.
700 1 $aCARVALHO, A. B. da S.
700 1 $aFONSECA, J. F. da
700 1 $aBRANDÃO, F. Z.
773 $tVeterinary Record, e1064, 2021.
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Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
14/12/2023 |
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Data da última atualização: |
14/12/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
LEMOS, I. M. L.; ROSADO, C. P.; TEODORO, A. J.; STEPHAN, M. P.; ROSA, J. S. da. |
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Afiliação: |
ISABELA DA MOTA LEAL LEMOS, UFF; CAROLYNE PIMENTEL ROSADO, UNIRIO; ANDERSON JUNGER TEODORO, CNPq; MARILIA PENTEADO STEPHAN, CTAA; JEANE SANTOS DA ROSA, CTAA. |
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Título: |
Evaluation of antioxidant activity and total phenolic compounds in yellow corn flour (Zea mays) and purple corn flour (Zea mays L.). |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
In: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS E NUTRIÇÃO, 15., 2023, Campinas. A revolução da ciência de alimentos e nutrição: alimentando o mundo de forma sustentável: caderno [eletrônico] de resumos. Campinas: Galoá, 2023. Pôster 168404; SLACAN. |
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Idioma: |
Inglês |
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Notas: |
Pôster 166883; SLACAN. |
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Conteúdo: |
Corn is important as one of the main cereals cultivated in Brazil and in the world. Its adaptability to climate results in a variety of genotypes, which may have different levels of bioactive compounds. The aim of the study was to analyze the total phenolic content and antioxidant activity of yellow corn flour (FMA) and purple corn flour (FMR). The flours were purchased from local shops and aqueous extracts (FMAa and FMRa) and 70% ethanol extracts (FMAe and FMRe) were prepared in proportions of 1:10 (FMA) and 1:20 (FMR), which were used to quantify the content of total phenolics (Folin-Ciocalteu) and antioxidant activity (FRAP, ORAC, DPPH and ABTS+). Regarding total phenolics, FMAa (2.03±0.12mg% AGE) and FMAe (1.85±0.78mg% AGE) did not differ from each other (p=0.9993). However, in FMR the use of water optimized the extraction of phenolics when compared to 70% ethanol (respectively 19.61 and 15.42 mg% AGE). Regarding the antioxidant analysis, the flours presented the following results: FMRe (ORAC: 34.56±14.2µM TE/g; FRAP: 70.74±2.1813µM Fe2SO4/g; ABTS: 238.67±2.08µM Trolox/g and DPPH: 403.92±0.58µM Trolox/g); FMRa (ORAC: 51.82±12.2µM TE/g; FRAP: 65.53±3.72µM Fe2SO4/g; ABTS: 302.67±8.52µM Trolox/g and DPPH: 402.25±1.32µM Trolox/g); FMAe (ORAC: 7.81±2.80µM TE/g; FRAP: 2.52±0.13µM Fe2SO4/g; ABTS: 13.78±0.78µM Trolox/g and DPPH: 34.11.92±0. 29µM Trolox/g) and FMAa (ORAC: 6.16±4.56µM TE/g; FRAP: 2.3±0.39µM Fe2SO4/g; ABTS: 16.8±1.58µM Trolox/g and DPPH: 36. 26±0.25µM Trolox/g). Thus, FMR showed a significantly higher content of phenolic compounds (p<0.0001) and antioxidant activity (p<0.0001) compared to FMA, regardless of the solvent and methods analyzed. This behavior can be attributed to the high content of anthocyanins present in purple corn, which are more potent in terms of antioxidant activity when compared to the carotenoids present in yellow corn. Thus, the data suggest a high antioxidant power of FMR, which may be an important ally in the production of new products. MenosCorn is important as one of the main cereals cultivated in Brazil and in the world. Its adaptability to climate results in a variety of genotypes, which may have different levels of bioactive compounds. The aim of the study was to analyze the total phenolic content and antioxidant activity of yellow corn flour (FMA) and purple corn flour (FMR). The flours were purchased from local shops and aqueous extracts (FMAa and FMRa) and 70% ethanol extracts (FMAe and FMRe) were prepared in proportions of 1:10 (FMA) and 1:20 (FMR), which were used to quantify the content of total phenolics (Folin-Ciocalteu) and antioxidant activity (FRAP, ORAC, DPPH and ABTS+). Regarding total phenolics, FMAa (2.03±0.12mg% AGE) and FMAe (1.85±0.78mg% AGE) did not differ from each other (p=0.9993). However, in FMR the use of water optimized the extraction of phenolics when compared to 70% ethanol (respectively 19.61 and 15.42 mg% AGE). Regarding the antioxidant analysis, the flours presented the following results: FMRe (ORAC: 34.56±14.2µM TE/g; FRAP: 70.74±2.1813µM Fe2SO4/g; ABTS: 238.67±2.08µM Trolox/g and DPPH: 403.92±0.58µM Trolox/g); FMRa (ORAC: 51.82±12.2µM TE/g; FRAP: 65.53±3.72µM Fe2SO4/g; ABTS: 302.67±8.52µM Trolox/g and DPPH: 402.25±1.32µM Trolox/g); FMAe (ORAC: 7.81±2.80µM TE/g; FRAP: 2.52±0.13µM Fe2SO4/g; ABTS: 13.78±0.78µM Trolox/g and DPPH: 34.11.92±0. 29µM Trolox/g) and FMAa (ORAC: 6.16±4.56µM TE/g; FRAP: 2.3±0.39µM Fe2SO4/g; ABTS: 16.8±1.58µM Trolox/g and DPPH: 36. 26±0.25µM Trolox/g). Th... Mostrar Tudo |
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Palavras-Chave: |
By-products. |
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Thesagro: |
Cereal. |
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Thesaurus NAL: |
Bioactive compounds. |
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Categoria do assunto: |
Q Alimentos e Nutrição Humana |
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Marc: |
LEADER 02926nam a2200205 a 4500
001 2159721
005 2023-12-14
008 2023 bl uuuu u00u1 u #d
100 1 $aLEMOS, I. M. L.
245 $aEvaluation of antioxidant activity and total phenolic compounds in yellow corn flour (Zea mays) and purple corn flour (Zea mays L.).$h[electronic resource]
260 $aIn: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS E NUTRIÇÃO, 15., 2023, Campinas. A revolução da ciência de alimentos e nutrição: alimentando o mundo de forma sustentável: caderno [eletrônico] de resumos. Campinas: Galoá, 2023. Pôster 168404; SLACAN.$c2023
500 $aPôster 166883; SLACAN.
520 $aCorn is important as one of the main cereals cultivated in Brazil and in the world. Its adaptability to climate results in a variety of genotypes, which may have different levels of bioactive compounds. The aim of the study was to analyze the total phenolic content and antioxidant activity of yellow corn flour (FMA) and purple corn flour (FMR). The flours were purchased from local shops and aqueous extracts (FMAa and FMRa) and 70% ethanol extracts (FMAe and FMRe) were prepared in proportions of 1:10 (FMA) and 1:20 (FMR), which were used to quantify the content of total phenolics (Folin-Ciocalteu) and antioxidant activity (FRAP, ORAC, DPPH and ABTS+). Regarding total phenolics, FMAa (2.03±0.12mg% AGE) and FMAe (1.85±0.78mg% AGE) did not differ from each other (p=0.9993). However, in FMR the use of water optimized the extraction of phenolics when compared to 70% ethanol (respectively 19.61 and 15.42 mg% AGE). Regarding the antioxidant analysis, the flours presented the following results: FMRe (ORAC: 34.56±14.2µM TE/g; FRAP: 70.74±2.1813µM Fe2SO4/g; ABTS: 238.67±2.08µM Trolox/g and DPPH: 403.92±0.58µM Trolox/g); FMRa (ORAC: 51.82±12.2µM TE/g; FRAP: 65.53±3.72µM Fe2SO4/g; ABTS: 302.67±8.52µM Trolox/g and DPPH: 402.25±1.32µM Trolox/g); FMAe (ORAC: 7.81±2.80µM TE/g; FRAP: 2.52±0.13µM Fe2SO4/g; ABTS: 13.78±0.78µM Trolox/g and DPPH: 34.11.92±0. 29µM Trolox/g) and FMAa (ORAC: 6.16±4.56µM TE/g; FRAP: 2.3±0.39µM Fe2SO4/g; ABTS: 16.8±1.58µM Trolox/g and DPPH: 36. 26±0.25µM Trolox/g). Thus, FMR showed a significantly higher content of phenolic compounds (p<0.0001) and antioxidant activity (p<0.0001) compared to FMA, regardless of the solvent and methods analyzed. This behavior can be attributed to the high content of anthocyanins present in purple corn, which are more potent in terms of antioxidant activity when compared to the carotenoids present in yellow corn. Thus, the data suggest a high antioxidant power of FMR, which may be an important ally in the production of new products.
650 $aBioactive compounds
650 $aCereal
653 $aBy-products
700 1 $aROSADO, C. P.
700 1 $aTEODORO, A. J.
700 1 $aSTEPHAN, M. P.
700 1 $aROSA, J. S. da
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