| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
30/10/2021 |
|
Data da última atualização: |
03/10/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
OLIVEIRA, E. R.; FAYER, L.; ZANETTE, R. S. S.; LADEIRA, L. O.; OLIVEIRA, L. F. C. de; MARANDUBA, C. M. C.; BRANDAO, H. de M.; MUNK, M. |
|
Afiliação: |
EDUARDA R. OLIVEIRA, Universidade Federal de Juiz de Fora; LEONARA FAYER, Universidade Federal de Juiz de Fora; RAFAELLA S. S. ZANETTE, Universidade Federal de Juiz de Fora; LUIZ O. LADEIRA, Universidade Federal de Minas Gerais; LUIZ F. C. DE OLIVEIRA, Universidade Federal de Juiz de Fora; CARLOS M. C. MARANDUBA, Universidade Federal de Juiz de Fora; HUMBERTO DE MELLO BRANDAO, CNPGL; MICHELE MUNK, Universidade Federal de Juiz de Fora. |
|
Título: |
Cytocompatibility of carboxylated multi-wall carbon nanotubes in stem cells from human exfoliated deciduous teeth. |
|
Ano de publicação: |
2022 |
|
Fonte/Imprenta: |
Nanotechnology, v. 33, n. 6, 065101, 2022. |
|
DOI: |
https://doi.org/10.1088/1361-6528/ac335b |
|
Idioma: |
Inglês |
|
Conteúdo: |
Carboxylated Multi-wall Carbon Nanotube (MWCNT-COOH) presents unique properties due to nanoscale dimensions and permits a broad range of applications in different fields, such as bone tissue engineering and regenerative medicine. However, the cytocompatibility of MWCNT-COOH with human stem cells is poorly understood. Thus, studies elucidating how MWCNT-COOH affects human stem cell viability are essential to a safer application of nanotechnologies. Using stem cells from the human exfoliated deciduous teeth model, we have evaluated the effects of MWCNT-COOH on cell viability, oxidative cell stress, and DNA integrity. Results demonstrated that despite the decreased metabolism of mitochondria, MWCNT-COOH had no toxicity against stem cells. Cells maintained viability after MWCNT-COOH exposure. MWCNTCOOH did not alter the superoxide dismutase activity and did not cause genotoxic effects. The present findings are relevant to the potential application of MWCNT-COOH in the tissue engineering and regenerative medicine fields. |
|
Palavras-Chave: |
Células-tronco; Engenharia de tecido; Estudo in vitro; Nanotecnologia. |
|
Thesagro: |
Carbono. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1135728/1/Cytocompatibility-of-carboxylated-multi-wall-carbon-nanotubes-in-stem-cells-.pdf
|
|
Marc: |
LEADER 01878naa a2200277 a 4500
001 2135728
005 2023-10-03
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1088/1361-6528/ac335b$2DOI
100 1 $aOLIVEIRA, E. R.
245 $aCytocompatibility of carboxylated multi-wall carbon nanotubes in stem cells from human exfoliated deciduous teeth.$h[electronic resource]
260 $c2022
520 $aCarboxylated Multi-wall Carbon Nanotube (MWCNT-COOH) presents unique properties due to nanoscale dimensions and permits a broad range of applications in different fields, such as bone tissue engineering and regenerative medicine. However, the cytocompatibility of MWCNT-COOH with human stem cells is poorly understood. Thus, studies elucidating how MWCNT-COOH affects human stem cell viability are essential to a safer application of nanotechnologies. Using stem cells from the human exfoliated deciduous teeth model, we have evaluated the effects of MWCNT-COOH on cell viability, oxidative cell stress, and DNA integrity. Results demonstrated that despite the decreased metabolism of mitochondria, MWCNT-COOH had no toxicity against stem cells. Cells maintained viability after MWCNT-COOH exposure. MWCNTCOOH did not alter the superoxide dismutase activity and did not cause genotoxic effects. The present findings are relevant to the potential application of MWCNT-COOH in the tissue engineering and regenerative medicine fields.
650 $aCarbono
653 $aCélulas-tronco
653 $aEngenharia de tecido
653 $aEstudo in vitro
653 $aNanotecnologia
700 1 $aFAYER, L.
700 1 $aZANETTE, R. S. S.
700 1 $aLADEIRA, L. O.
700 1 $aOLIVEIRA, L. F. C. de
700 1 $aMARANDUBA, C. M. C.
700 1 $aBRANDAO, H. de M.
700 1 $aMUNK, M.
773 $tNanotechnology$gv. 33, n. 6, 065101, 2022.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
31/03/2009 |
|
Data da última atualização: |
30/06/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
Internacional - A |
|
Autoria: |
LEÓN, M. G.; BECERRA, C. H.; FREITAS-ASTÚA, J.; SALAROLI, R. B.; KITAJIMA, E. W. |
|
Afiliação: |
M. G. León, CORPOICA; C. H. Becerra, ICA; Juliana Freitas-Ástua, CNPMF; R. B. Salaroli, ESALQ; Elliot Watanabe Kitajima, ESALQ. |
|
Título: |
Natural infection of Swinglea glutinosa by the Citrus leprosis virus cytoplasmic type (CiLV-C) in Colombia. |
|
Ano de publicação: |
2008 |
|
Fonte/Imprenta: |
Plant Disease, v.92, n.9, p.1364, 2008. |
|
Idioma: |
Inglês |
|
Notas: |
DOI: 10.1094/PDIS-92-9-1364C |
|
Conteúdo: |
Swinglea glutinosa (Blanco) Merr., a perennial plant in the family Rutaceae, is originally from southeast Asia but which is now grown worldwide. In Colombia, it is used as an ornamental and principally as a living fence around rural properties and farms in several regions of the country. Citrus leprosis virus cytoplasmic type (CiLV-C) was recently detected in orange groves of the Colombian Piedmont eastern plains, an area known as the Llanos Orientales (2). Because of the potential for country-wide infection of citrus, some measures are being taken to avoid CiLV-C spread to other regions of Colombia. Further surveys made from June to December 2005 to evaluate the extent of the spread of CiLV-C in the Llanos Orientales revealed some plants in S. glutinosa hedges surrounding citrus orchards exhibiting chlorotic spots and ringspots of varied size on the leaves, similar to those caused by CiLV-C on sweet oranges leaves. These plants were found near citrus orchards in the municipalities of Guamal and in some urban areas of Villavicencio City in the Meta Department. The possibility that these symptoms were caused by CiLV-C was investigated soon after sample collection by the same procedures as described previously for sweet orange (2). In the leaf lesions of S. glutinosa, typical bacilliform particles and dense cytoplasmic viroplasm were found with electron microscopy. Total RNA extracted from symptomatic leaves was subjected to reverse transcription-PCR (RT) using primers (Fwd. 5'GATACGGGACGCATAACA-3'/Rev. 5'-TTCTGGCTCAACATCTGG-3') that specifically amplify a region within the CiLV-C putative methyltransferase gene and this yielded a single fragment of the expected 402 bp (3). Analysis of the consensus sequence derived from 20 RT-PCR products (GenBank Accession No. EU689106) showed 96% nucleotide and 92% amino acid sequence identity to the sequence of a Brazilian CiLV-C isolate (GenBank Accession Nos. DQ352194.1 and YP_654565.1), respectively. Recently, published work described mite transmission of CiLV-C to some nonrutaceous plants (1), but to our knowledge, this is the first report of a nonCitrus rutaceous plant naturally infected by CiLV-C. Mites found in citrus orchards and previously identified as Brevipalpus phoenicis (Geijskes) (2), which are likely the most important vector of CiLV-C in citrus in Colombia, were observed feeding on healthy and symptomatic S. glutinosa, indicating that S. glutinosa is a host for B. phoenicis. Because the use of S. glutinosa as a living fence or hedge is a common practice in Colombia, CiLV-C-infected S. glutinosa plants may play a role in the epidemiology of leprosis in commercial citrus by serving as an inoculum source for this lethal virus. MenosSwinglea glutinosa (Blanco) Merr., a perennial plant in the family Rutaceae, is originally from southeast Asia but which is now grown worldwide. In Colombia, it is used as an ornamental and principally as a living fence around rural properties and farms in several regions of the country. Citrus leprosis virus cytoplasmic type (CiLV-C) was recently detected in orange groves of the Colombian Piedmont eastern plains, an area known as the Llanos Orientales (2). Because of the potential for country-wide infection of citrus, some measures are being taken to avoid CiLV-C spread to other regions of Colombia. Further surveys made from June to December 2005 to evaluate the extent of the spread of CiLV-C in the Llanos Orientales revealed some plants in S. glutinosa hedges surrounding citrus orchards exhibiting chlorotic spots and ringspots of varied size on the leaves, similar to those caused by CiLV-C on sweet oranges leaves. These plants were found near citrus orchards in the municipalities of Guamal and in some urban areas of Villavicencio City in the Meta Department. The possibility that these symptoms were caused by CiLV-C was investigated soon after sample collection by the same procedures as described previously for sweet orange (2). In the leaf lesions of S. glutinosa, typical bacilliform particles and dense cytoplasmic viroplasm were found with electron microscopy. Total RNA extracted from symptomatic leaves was subjected to reverse transcription-PCR (RT) using primers (Fwd. 5... Mostrar Tudo |
|
Thesagro: |
Ácaro; Doença de Planta; Leprose; Vírus. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 03395naa a2200229 a 4500
001 1655641
005 2023-06-30
008 2008 bl uuuu u00u1 u #d
100 1 $aLEÓN, M. G.
245 $aNatural infection of Swinglea glutinosa by the Citrus leprosis virus cytoplasmic type (CiLV-C) in Colombia.$h[electronic resource]
260 $c2008
500 $aDOI: 10.1094/PDIS-92-9-1364C
520 $aSwinglea glutinosa (Blanco) Merr., a perennial plant in the family Rutaceae, is originally from southeast Asia but which is now grown worldwide. In Colombia, it is used as an ornamental and principally as a living fence around rural properties and farms in several regions of the country. Citrus leprosis virus cytoplasmic type (CiLV-C) was recently detected in orange groves of the Colombian Piedmont eastern plains, an area known as the Llanos Orientales (2). Because of the potential for country-wide infection of citrus, some measures are being taken to avoid CiLV-C spread to other regions of Colombia. Further surveys made from June to December 2005 to evaluate the extent of the spread of CiLV-C in the Llanos Orientales revealed some plants in S. glutinosa hedges surrounding citrus orchards exhibiting chlorotic spots and ringspots of varied size on the leaves, similar to those caused by CiLV-C on sweet oranges leaves. These plants were found near citrus orchards in the municipalities of Guamal and in some urban areas of Villavicencio City in the Meta Department. The possibility that these symptoms were caused by CiLV-C was investigated soon after sample collection by the same procedures as described previously for sweet orange (2). In the leaf lesions of S. glutinosa, typical bacilliform particles and dense cytoplasmic viroplasm were found with electron microscopy. Total RNA extracted from symptomatic leaves was subjected to reverse transcription-PCR (RT) using primers (Fwd. 5'GATACGGGACGCATAACA-3'/Rev. 5'-TTCTGGCTCAACATCTGG-3') that specifically amplify a region within the CiLV-C putative methyltransferase gene and this yielded a single fragment of the expected 402 bp (3). Analysis of the consensus sequence derived from 20 RT-PCR products (GenBank Accession No. EU689106) showed 96% nucleotide and 92% amino acid sequence identity to the sequence of a Brazilian CiLV-C isolate (GenBank Accession Nos. DQ352194.1 and YP_654565.1), respectively. Recently, published work described mite transmission of CiLV-C to some nonrutaceous plants (1), but to our knowledge, this is the first report of a nonCitrus rutaceous plant naturally infected by CiLV-C. Mites found in citrus orchards and previously identified as Brevipalpus phoenicis (Geijskes) (2), which are likely the most important vector of CiLV-C in citrus in Colombia, were observed feeding on healthy and symptomatic S. glutinosa, indicating that S. glutinosa is a host for B. phoenicis. Because the use of S. glutinosa as a living fence or hedge is a common practice in Colombia, CiLV-C-infected S. glutinosa plants may play a role in the epidemiology of leprosis in commercial citrus by serving as an inoculum source for this lethal virus.
650 $aÁcaro
650 $aDoença de Planta
650 $aLeprose
650 $aVírus
700 1 $aBECERRA, C. H.
700 1 $aFREITAS-ASTÚA, J.
700 1 $aSALAROLI, R. B.
700 1 $aKITAJIMA, E. W.
773 $tPlant Disease$gv.92, n.9, p.1364, 2008.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
