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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
20/08/2021 |
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Data da última atualização: |
20/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DIAS, M. A. M.; BOMFIM, C. S. G.; RODRIGUES, D. R.; SILVA, A. F. da; SANTOS, J. C. S.; NASCIMENTO, T. R. do; MARTINS, L. M. V.; DANTAS, B. F.; RIBEIRO, P. R. de A.; FREITAS, A. D. S. de; FERNANDES JUNIOR, P. I. |
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Afiliação: |
MARCOS ANDRÉ MOURA DIAS, UNIVASF; CLAUDIA SILVA GOMES BOMFIM; DALILA RIBEIRO RODRIGUES, UEPB; ALEKSANDRO FERREIRA DA SILVA, UFRPE; JÉSSICA CAROLINE SOUZA SANTOS, UPE; TAILANE RIBEIRO DO NASCIMENTO, UNEB; LINDETE MÍRIA VIEIRA MARTINS, UNEB; BARBARA FRANCA DANTAS, CPATSA; PAULA ROSE DE ALMEIDA RIBEIRO; ANA DOLORES SANTIAGO DE FREITAS, UFRPE; PAULO IVAN FERNANDES JUNIOR, CPATSA. |
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Título: |
Paraburkholderia spp. are the main rhizobial microsymbionts of Mimosa tenuiflora (Willd.) Poir. in soils of the Brazilian tropical dry forests (Caatinga biome). |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Systematic and Applied Microbiology, v. 44, 2021. |
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DOI: |
https://doi.org/10.1016/j.syapm.2021.126208 |
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Idioma: |
Inglês |
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Conteúdo: |
Mimosa tenuiflora (Willd.) Poir. is widespread in southern and central American drylands, but little information is available concerning its associated rhizobia. Therefore, this study aimed to characterize M. tenuiflora rhizobia from soils of the tropical dry forests (Caatinga) in Pernambuco State, Brazil, at the molecular and symbiotic levels. Soil samples of pristine Caatinga areas in four municipalities were used to grow M. tenuiflora. First, the bacteria from root nodules were subjected to nodC/nifH gene amplification, and the bacteria positive for both genes had the 16S rRNA gene sequenced. Then, ten strains were evaluated using recA, gyrB, and nodC gene sequences, and seven of them had their symbiotic efficiency assessed. Thirty-two strains were obtained and 22 of them were nodC/nifH positive. Twenty strains clustered within Paraburkholderia and two within Rhizobium by 16S rRNA gene sequencing. The beta-rhizobia were similar to P. phenoliruptrix (12) and P. diazotrophica (8). Both alpha-rhizobia were closely related to R. miluonense. The recA + gyrB phylogenetic analysis clustered four and five strains within the P. phenoliruptrix and P. diazotrophica branches, respectively, but they were somewhat divergent to the 16S rRNA phylogeny. For Rhizobium sp. ESA 637, the recA + gyrB phylogeny clustered the strain with R. jaguaris. The nodC phylogeny indicated that ESA 626, ESA 629, and ESA 630 probably represented a new symbiovar branch. The inoculation assay showed high symbiotic efficiency for all tested strains. The results indicated high genetic diversity and efficiency of M. tenuiflora rhizobia in Brazilian drylands and included P. phenoliruptrix-like bacteria in the list of efficient beta-rhizobia in the Caatinga biome. MenosMimosa tenuiflora (Willd.) Poir. is widespread in southern and central American drylands, but little information is available concerning its associated rhizobia. Therefore, this study aimed to characterize M. tenuiflora rhizobia from soils of the tropical dry forests (Caatinga) in Pernambuco State, Brazil, at the molecular and symbiotic levels. Soil samples of pristine Caatinga areas in four municipalities were used to grow M. tenuiflora. First, the bacteria from root nodules were subjected to nodC/nifH gene amplification, and the bacteria positive for both genes had the 16S rRNA gene sequenced. Then, ten strains were evaluated using recA, gyrB, and nodC gene sequences, and seven of them had their symbiotic efficiency assessed. Thirty-two strains were obtained and 22 of them were nodC/nifH positive. Twenty strains clustered within Paraburkholderia and two within Rhizobium by 16S rRNA gene sequencing. The beta-rhizobia were similar to P. phenoliruptrix (12) and P. diazotrophica (8). Both alpha-rhizobia were closely related to R. miluonense. The recA + gyrB phylogenetic analysis clustered four and five strains within the P. phenoliruptrix and P. diazotrophica branches, respectively, but they were somewhat divergent to the 16S rRNA phylogeny. For Rhizobium sp. ESA 637, the recA + gyrB phylogeny clustered the strain with R. jaguaris. The nodC phylogeny indicated that ESA 626, ESA 629, and ESA 630 probably represented a new symbiovar branch. The inoculation assay showed high symb... Mostrar Tudo |
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Palavras-Chave: |
Bioma Caatinga; Fixação biológica de nitrogênio; Região semiárida brasileira; Rizobio. |
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Thesagro: |
Caatinga; Floresta Tropical; Inoculante; Solo. |
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Thesaurus Nal: |
Soil. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02903naa a2200361 a 4500
001 2133773
005 2021-08-20
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.syapm.2021.126208$2DOI
100 1 $aDIAS, M. A. M.
245 $aParaburkholderia spp. are the main rhizobial microsymbionts of Mimosa tenuiflora (Willd.) Poir. in soils of the Brazilian tropical dry forests (Caatinga biome).$h[electronic resource]
260 $c2021
520 $aMimosa tenuiflora (Willd.) Poir. is widespread in southern and central American drylands, but little information is available concerning its associated rhizobia. Therefore, this study aimed to characterize M. tenuiflora rhizobia from soils of the tropical dry forests (Caatinga) in Pernambuco State, Brazil, at the molecular and symbiotic levels. Soil samples of pristine Caatinga areas in four municipalities were used to grow M. tenuiflora. First, the bacteria from root nodules were subjected to nodC/nifH gene amplification, and the bacteria positive for both genes had the 16S rRNA gene sequenced. Then, ten strains were evaluated using recA, gyrB, and nodC gene sequences, and seven of them had their symbiotic efficiency assessed. Thirty-two strains were obtained and 22 of them were nodC/nifH positive. Twenty strains clustered within Paraburkholderia and two within Rhizobium by 16S rRNA gene sequencing. The beta-rhizobia were similar to P. phenoliruptrix (12) and P. diazotrophica (8). Both alpha-rhizobia were closely related to R. miluonense. The recA + gyrB phylogenetic analysis clustered four and five strains within the P. phenoliruptrix and P. diazotrophica branches, respectively, but they were somewhat divergent to the 16S rRNA phylogeny. For Rhizobium sp. ESA 637, the recA + gyrB phylogeny clustered the strain with R. jaguaris. The nodC phylogeny indicated that ESA 626, ESA 629, and ESA 630 probably represented a new symbiovar branch. The inoculation assay showed high symbiotic efficiency for all tested strains. The results indicated high genetic diversity and efficiency of M. tenuiflora rhizobia in Brazilian drylands and included P. phenoliruptrix-like bacteria in the list of efficient beta-rhizobia in the Caatinga biome.
650 $aSoil
650 $aCaatinga
650 $aFloresta Tropical
650 $aInoculante
650 $aSolo
653 $aBioma Caatinga
653 $aFixação biológica de nitrogênio
653 $aRegião semiárida brasileira
653 $aRizobio
700 1 $aBOMFIM, C. S. G.
700 1 $aRODRIGUES, D. R.
700 1 $aSILVA, A. F. da
700 1 $aSANTOS, J. C. S.
700 1 $aNASCIMENTO, T. R. do
700 1 $aMARTINS, L. M. V.
700 1 $aDANTAS, B. F.
700 1 $aRIBEIRO, P. R. de A.
700 1 $aFREITAS, A. D. S. de
700 1 $aFERNANDES JUNIOR, P. I.
773 $tSystematic and Applied Microbiology$gv. 44, 2021.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
11/01/1994 |
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Data da última atualização: |
14/09/2020 |
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Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
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Autoria: |
NUNES, A. M. L. |
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Título: |
Ocorrência da queima-do-fio Pellicularia koleroga em pimenta-do-reino no municipio de Porto Velho-RO. |
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Ano de publicação: |
1989 |
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Fonte/Imprenta: |
Porto Velho: EMBRAPA-UEPAE Porto Velho, 1989. |
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Páginas: |
3 p. |
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Série: |
(EMBRAPA-UEPAE Porto Velho. Comunicado téccnico, 69). |
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Idioma: |
Português |
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Conteúdo: |
Danos e perdas. Sintomas. O patógeno e condições predisponente à infecção. Teste de patogenicidade. |
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Palavras-Chave: |
Amazônia Ocidental; Brasil; Disease; Fungus; Pimenta-do-reino; Porto Velho; Porto Velho (RO); Rondonia; Western Amazon. |
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Thesagro: |
Doença; Doença de Planta; Fungo; Pellicularia Koleroga; Pimenta do Reino; Piper Nigrum; Queima do Fio. |
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Thesaurus NAL: |
Amazonia; black pepper; Fungal diseases of plants. |
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Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 01110nam a2200361 a 4500
001 1698033
005 2020-09-14
008 1989 bl uuuu u0uu1 u #d
100 1 $aNUNES, A. M. L.
245 $aOcorrência da queima-do-fio Pellicularia koleroga em pimenta-do-reino no municipio de Porto Velho-RO.
260 $aPorto Velho: EMBRAPA-UEPAE Porto Velho$c1989
300 $a3 p.
490 $a(EMBRAPA-UEPAE Porto Velho. Comunicado téccnico, 69).
520 $aDanos e perdas. Sintomas. O patógeno e condições predisponente à infecção. Teste de patogenicidade.
650 $aAmazonia
650 $ablack pepper
650 $aFungal diseases of plants
650 $aDoença
650 $aDoença de Planta
650 $aFungo
650 $aPellicularia Koleroga
650 $aPimenta do Reino
650 $aPiper Nigrum
650 $aQueima do Fio
653 $aAmazônia Ocidental
653 $aBrasil
653 $aDisease
653 $aFungus
653 $aPimenta-do-reino
653 $aPorto Velho
653 $aPorto Velho (RO)
653 $aRondonia
653 $aWestern Amazon
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