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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
21/07/2021 |
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Data da última atualização: |
21/07/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, C. M. de J.; DIAS, R. de C. S.; MELO, N. F. de. |
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Afiliação: |
CARLA MARIA DE JESUS SILVA; RITA DE CASSIA SOUZA DIAS, CPATSA; NATONIEL FRANKLIN DE MELO, CPATSA. |
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Título: |
The effect of temperature and growth regulators on callus induction in watermelon anthers. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Brazilian Archives of Biology and Technology, v. 64, e21180505, 2021. |
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ISSN: |
1678-4324 |
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DOI: |
https://doi.org/10.1590/1678-4324-2021180505 |
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Idioma: |
Inglês |
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Conteúdo: |
Callus induction is one of the pathways required for haploid plant regeneration through anther culture. Pollen viability, as well as the effect of growth regulators and cold pretreatment on anthers of two watermelon lines (Smile and Sugar Baby) to induce callus formation were herein evaluated. Pollen viability was estimated through the staining technique using 2% acetic carmine. Male flower buds were collected and disinfested to allow removal anthers. These anthers were placed on Murashige and Skoog medium, which was supplemented with 2,4-dichlorophenoxyacetic (2,4-D) at 0.0, 0.5, 1.0, 2.0 or 5.0 ?M or with 6-benzylaminopurine at 0.0, 0.5, 1.0, 1.5, or 2.0 ?M, in combination with 2.0 ?M of 2,4-dichlorophenoxyacetic. Anthers were pretreated at 4 °C, for two days and then placed in vitro. Both watermelon lines provided high pollen viability rates (from 93 to 98%). The 2.0 and 5.0 ?M concentrations of 2,4-D stimulated higher friable callus formation. The optimal concentration of 2,4-D was estimated at 3.78 ?M and 4.17 ?M, which had callus induction rates of 64% and 52%, respectively. The combination of 2.0 ?M of 2,4-D and 6-benzylaminopurine did not lead to increased anther response to callus induction. The pre-treatment applied to flower buds at 4 °C enabled callus induction and the anther response to callus induction was genotype-dependent. |
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Palavras-Chave: |
Calogênese de anteras; Viabilidade do pólen. |
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Thesagro: |
Citrullus Lanatus; Cucurbitaceae; Fruta Cucurbitácea; Melancia; Regulador de Crescimento. |
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Thesaurus Nal: |
Growth regulators; Watermelons. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/224590/1/The-effect-of-temperature-and-growth-regulator-2021.pdf
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Marc: |
LEADER 02236naa a2200277 a 4500
001 2133062
005 2021-07-21
008 2021 bl uuuu u00u1 u #d
022 $a1678-4324
024 7 $ahttps://doi.org/10.1590/1678-4324-2021180505$2DOI
100 1 $aSILVA, C. M. de J.
245 $aThe effect of temperature and growth regulators on callus induction in watermelon anthers.$h[electronic resource]
260 $c2021
520 $aCallus induction is one of the pathways required for haploid plant regeneration through anther culture. Pollen viability, as well as the effect of growth regulators and cold pretreatment on anthers of two watermelon lines (Smile and Sugar Baby) to induce callus formation were herein evaluated. Pollen viability was estimated through the staining technique using 2% acetic carmine. Male flower buds were collected and disinfested to allow removal anthers. These anthers were placed on Murashige and Skoog medium, which was supplemented with 2,4-dichlorophenoxyacetic (2,4-D) at 0.0, 0.5, 1.0, 2.0 or 5.0 ?M or with 6-benzylaminopurine at 0.0, 0.5, 1.0, 1.5, or 2.0 ?M, in combination with 2.0 ?M of 2,4-dichlorophenoxyacetic. Anthers were pretreated at 4 °C, for two days and then placed in vitro. Both watermelon lines provided high pollen viability rates (from 93 to 98%). The 2.0 and 5.0 ?M concentrations of 2,4-D stimulated higher friable callus formation. The optimal concentration of 2,4-D was estimated at 3.78 ?M and 4.17 ?M, which had callus induction rates of 64% and 52%, respectively. The combination of 2.0 ?M of 2,4-D and 6-benzylaminopurine did not lead to increased anther response to callus induction. The pre-treatment applied to flower buds at 4 °C enabled callus induction and the anther response to callus induction was genotype-dependent.
650 $aGrowth regulators
650 $aWatermelons
650 $aCitrullus Lanatus
650 $aCucurbitaceae
650 $aFruta Cucurbitácea
650 $aMelancia
650 $aRegulador de Crescimento
653 $aCalogênese de anteras
653 $aViabilidade do pólen
700 1 $aDIAS, R. de C. S.
700 1 $aMELO, N. F. de
773 $tBrazilian Archives of Biology and Technology$gv. 64, e21180505, 2021.
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Embrapa Semiárido (CPATSA) |
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