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Registro Completo |
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Biblioteca(s): |
Embrapa Clima Temperado. |
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Data corrente: |
11/03/2021 |
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Data da última atualização: |
12/03/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VARGAS, D. P.; CORADIN, J. H.; FERREIRA, L. V.; DORNELES, A. O. S.; VALGAS, R. A.; PEREIRA, A. da S.; CASTRO, C. M.; DUTRA, L. F. |
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Afiliação: |
DAIANE PEIXOTO VARGAS; JULIANA HEY CORADIN, CPACT; LETÍCIA VANNI FERREIRA; ATHOS ODIN SEVERO DORNELES, UFPEL; RICARDO ALEXANDRE VALGAS, CPACT; ARIONE DA SILVA PEREIRA, CPACT; CAROLINE MARQUES CASTRO, CPACT; LEONARDO FERREIRA DUTRA, CPACT. |
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Título: |
Osmoregulators on in vitro encapsulation of potato "BRS Clara" buds. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Plant Cell Culture and Micropropagation, v. 16, e155, 2020. |
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Páginas: |
6 p. |
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ISSN: |
1808-9909 |
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Idioma: |
Inglês |
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Conteúdo: |
Synthetic or artificial seeds via encapsulated propagules could be used for in vitro regeneration and mass multiplication, for germplasm preservation and exchange of plant materials. An in vitro encapsulation of potato ?BRS Clara? buds efficient protocol was established. Lateral buds were immersed in a 4% sodium alginate matrix, dripped in CaCl2 for 20 minutes in different osmoregulatory agents: sucrose (8.72, 15.00, 21.28 and 30.00 g L-1) and mannitol (0.00, 11.63, 20.0, 28.37 and 40.0 g L-1). They were then held for 30 days at approximately 4°C and subsequently immersed in KNO3 for capsule decomplexing. Afterward, they were inoculated in MS medium with 3% sucrose and cultured in a growth room under irradiance of 43 μmol m-2 s-1, 16 hours-photoperiod and 25±2 °C temperature. Sucrose and mannitol at concentrations of 15 g L-1 and 20 g L-1, respectively, associated with the encapsulation matrix promoted 80% survival rate of ?BRS Clara? encapsulated buds. |
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Palavras-Chave: |
BRS Clara. |
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Thesagro: |
Batata. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/221827/1/Artigo-Plant-Cell-Cultura-Micropropagation.pdf
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Marc: |
LEADER 01666naa a2200253 a 4500
001 2130630
005 2021-03-12
008 2020 bl uuuu u00u1 u #d
022 $a1808-9909
100 1 $aVARGAS, D. P.
245 $aOsmoregulators on in vitro encapsulation of potato "BRS Clara" buds.$h[electronic resource]
260 $c2020
300 $a6 p.
520 $aSynthetic or artificial seeds via encapsulated propagules could be used for in vitro regeneration and mass multiplication, for germplasm preservation and exchange of plant materials. An in vitro encapsulation of potato ?BRS Clara? buds efficient protocol was established. Lateral buds were immersed in a 4% sodium alginate matrix, dripped in CaCl2 for 20 minutes in different osmoregulatory agents: sucrose (8.72, 15.00, 21.28 and 30.00 g L-1) and mannitol (0.00, 11.63, 20.0, 28.37 and 40.0 g L-1). They were then held for 30 days at approximately 4°C and subsequently immersed in KNO3 for capsule decomplexing. Afterward, they were inoculated in MS medium with 3% sucrose and cultured in a growth room under irradiance of 43 μmol m-2 s-1, 16 hours-photoperiod and 25±2 °C temperature. Sucrose and mannitol at concentrations of 15 g L-1 and 20 g L-1, respectively, associated with the encapsulation matrix promoted 80% survival rate of ?BRS Clara? encapsulated buds.
650 $aBatata
653 $aBRS Clara
700 1 $aCORADIN, J. H.
700 1 $aFERREIRA, L. V.
700 1 $aDORNELES, A. O. S.
700 1 $aVALGAS, R. A.
700 1 $aPEREIRA, A. da S.
700 1 $aCASTRO, C. M.
700 1 $aDUTRA, L. F.
773 $tPlant Cell Culture and Micropropagation$gv. 16, e155, 2020.
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Registro original: |
Embrapa Clima Temperado (CPACT) |
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| 1. |  | VARGAS, D. P.; CORADIN, J. H.; FERREIRA, L. V.; DORNELES, A. O. S.; VALGAS, R. A.; PEREIRA, A. da S.; CASTRO, C. M.; DUTRA, L. F. Osmoregulators on in vitro encapsulation of potato "BRS Clara" buds. Plant Cell Culture and Micropropagation, v. 16, e155, 2020. 6 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Clima Temperado. |
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| Registros recuperados : 1 | |
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