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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
28/12/2020 |
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Data da última atualização: |
28/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, L. N. B. de; DIAS, N. D. S. C.; SANTANA, V. de O.; SILVEIRA, L. A.; MEIRA, M. R.; SANTOS, E. S. L. dos; FALEIRO, F. G.; CERQUEIRA-SILVA, C. B. M. |
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Afiliação: |
FABIO GELAPE FALEIRO, CPAC. |
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Título: |
Amplification test and selection of markers analogue to resistance genes in species and commercial varieties of Passiflora spp. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Multi-Science Journal, v. 3, n. 1, 2020. |
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Páginas: |
p. 61-75 |
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Idioma: |
Português |
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Conteúdo: |
BSTRACT. Brazil is highlighted as the largest producer and consumer of passion fruit (Passiflora) and holder of representative biodiversity. Studies dedicated to the genetic characterization of the genus in order to understand the existing diversity are important for the advancement of genetic improvement and conservation programs. The aim of this study was to select combinations of RGA (Resistance Genes Analogs) primers for commercial passion fruit species and varieties. To this end, amplification tests were performed on a PCR platform in 17 combinations of RGA primers for eight species and 12 commercial varieties of passion fruit from the Banco Ativo de Germoplasma Flor da Paixão. All combinations of primers have generated amplification products in at least one of the Passiflora samples. The number of combinations that generated amplification products varied from seven to 12 in the species and from three to eight in the varieties. Three to 12 combinations generated amplification products for the samples, emphasizing that the RGA markers were efficient in accessing genomic loci in the species and varieties of passion fruit. The variation of efficient combinations in each of the species and varieties attested to the importance of this research in the preliminary stages of genetic studies as auxiliary tools in conservation and genetic improvement programs. However, this study made it possible to identify combinations of primers to be prioritized in inter and intraspecific genetic characterizations of passion fruit MenosBSTRACT. Brazil is highlighted as the largest producer and consumer of passion fruit (Passiflora) and holder of representative biodiversity. Studies dedicated to the genetic characterization of the genus in order to understand the existing diversity are important for the advancement of genetic improvement and conservation programs. The aim of this study was to select combinations of RGA (Resistance Genes Analogs) primers for commercial passion fruit species and varieties. To this end, amplification tests were performed on a PCR platform in 17 combinations of RGA primers for eight species and 12 commercial varieties of passion fruit from the Banco Ativo de Germoplasma Flor da Paixão. All combinations of primers have generated amplification products in at least one of the Passiflora samples. The number of combinations that generated amplification products varied from seven to 12 in the species and from three to eight in the varieties. Three to 12 combinations generated amplification products for the samples, emphasizing that the RGA markers were efficient in accessing genomic loci in the species and varieties of passion fruit. The variation of efficient combinations in each of the species and varieties attested to the importance of this research in the preliminary stages of genetic studies as auxiliary tools in conservation and genetic improvement programs. However, this study made it possible to identify combinations of primers to be prioritized in inter and intraspecific gen... Mostrar Tudo |
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Thesagro: |
Banco de Germoplasma; Passifloraceae; Seleção Genética. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/219730/1/FABIO-FALEIRO-Amplification-test-and-selection-of-markers-analog.pdf
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Marc: |
LEADER 02326naa a2200253 a 4500
001 2128776
005 2020-12-28
008 2020 bl uuuu u00u1 u #d
100 1 $aSOUZA, L. N. B. de
245 $aAmplification test and selection of markers analogue to resistance genes in species and commercial varieties of Passiflora spp.$h[electronic resource]
260 $c2020
300 $ap. 61-75
520 $aBSTRACT. Brazil is highlighted as the largest producer and consumer of passion fruit (Passiflora) and holder of representative biodiversity. Studies dedicated to the genetic characterization of the genus in order to understand the existing diversity are important for the advancement of genetic improvement and conservation programs. The aim of this study was to select combinations of RGA (Resistance Genes Analogs) primers for commercial passion fruit species and varieties. To this end, amplification tests were performed on a PCR platform in 17 combinations of RGA primers for eight species and 12 commercial varieties of passion fruit from the Banco Ativo de Germoplasma Flor da Paixão. All combinations of primers have generated amplification products in at least one of the Passiflora samples. The number of combinations that generated amplification products varied from seven to 12 in the species and from three to eight in the varieties. Three to 12 combinations generated amplification products for the samples, emphasizing that the RGA markers were efficient in accessing genomic loci in the species and varieties of passion fruit. The variation of efficient combinations in each of the species and varieties attested to the importance of this research in the preliminary stages of genetic studies as auxiliary tools in conservation and genetic improvement programs. However, this study made it possible to identify combinations of primers to be prioritized in inter and intraspecific genetic characterizations of passion fruit
650 $aBanco de Germoplasma
650 $aPassifloraceae
650 $aSeleção Genética
700 1 $aDIAS, N. D. S. C.
700 1 $aSANTANA, V. de O.
700 1 $aSILVEIRA, L. A.
700 1 $aMEIRA, M. R.
700 1 $aSANTOS, E. S. L. dos
700 1 $aFALEIRO, F. G.
700 1 $aCERQUEIRA-SILVA, C. B. M.
773 $tMulti-Science Journal$gv. 3, n. 1, 2020.
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Registro original: |
Embrapa Cerrados (CPAC) |
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| 1. |  | SOUZA, L. N. B. de; DIAS, N. D. S. C.; SANTANA, V. de O.; SILVEIRA, L. A.; MEIRA, M. R.; SANTOS, E. S. L. dos; FALEIRO, F. G.; CERQUEIRA-SILVA, C. B. M. Amplification test and selection of markers analogue to resistance genes in species and commercial varieties of Passiflora spp. Multi-Science Journal, v. 3, n. 1, 2020. p. 61-75| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
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