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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
08/12/2020 |
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Data da última atualização: |
08/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CATTANI, A. M.; FALAVIGNA, V. da S.; SILVEIRA, C. P.; BUFFON, V.; MARASCHIN, F. dos S.; PASQUALI, G.; REVERS, L. F. |
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Afiliação: |
AMANDA MALVESSI CATTANI, Graduate Program in Cell and Molecular Biology, Center for Biotechnology, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; VÍTOR DA SILVEIRA FALAVIGNA, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829 Köln, Germany; CAROLINA PEREIRA SILVEIRA, Laboratory of Plant Molecular Genetics, Embrapa Uva e Vinho, Bento Gonçalves, RS, Brazil; VANESSA BUFFON, CNPUV; FELIPE DOS SANTOS MARASCHIN, Departament of Botany, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; GIANCARLO PASQUALI, Graduate Program in Cell and Molecular Biology, Center for Biotechnology, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; LUIS FERNANDO REVERS, CNPUV. |
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Título: |
Type-B cytokinin response regulators link hormonal stimuli and molecular responses during the transition from endoto ecodormancy in apple buds. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Plant Cell Reports, v. 39, p. 1687-1703, 2020. |
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DOI: |
10.1007_s00299-020-02595-z.pdf |
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Idioma: |
Inglês |
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Conteúdo: |
Key Message Cytokinin together with MdoBRR1, MdoBRR8 and MdoBRR10 genes participate in the downregulation of MdoDAM1, contributing to the transition from endo- to ecodormancy in apple buds. Abstract The fnal step of cytokinin (CK) signaling pathway culminates in the activation of type-B response regulators (BRRs), important transcriptional factors in the modulation of CK-responsive genes. In this study, we performed a genomewide analysis aiming to identify apple BRR family members and understand their involvement in bud dormancy control. The investigation identifed ten MdoBRR protein-coding genes. A higher expression of three MdoBRR (MdoBRR1, MdoBRR9 and MdoBRR10) was observed in dormant buds in comparison to other developmental stages. Interestingly, in ecodormant buds these three MdoBRR genes were upregulated in a CK-dependent manner. Transcription profles, determined during dormancy cycle under feld and artifcially controlled conditions, revealed that MdoBRR1 and MdoBRR8 played important roles in the transition from endo- to ecodormancy, probably mediated by endogenous CK stimuli. The expression of MdoBRR7, MdoBRR9, and MdoBRR10 was induced in ecodormant buds exposed to warm temperatures, indicating a putative role in growth resumption after chilling requirement fulfllment. Contrasting expression patternsin vivo between MdoBRRs and MdoDAM1, an essential dormancy establishment regulator, were observed during dormancy cycle and in CK-treated buds. Thereafter, in vivo transactivation assays showed that CK stimuli combined with transient overexpression of MdoBRR1, MdoBRR8, and MdoBRR10 resulted in downregulation of the reporter gene gusA driven by the MdoDAM1 promoter. These pieces of evidences point to the integration of CK-triggered responses through MdoBRRs that are able to downregulate MdoDAM1, contributing to dormancy release in apple. Keywords MdoBRR · Cytokinin · Bud dormancy · MdoDAM1 · Apple MenosKey Message Cytokinin together with MdoBRR1, MdoBRR8 and MdoBRR10 genes participate in the downregulation of MdoDAM1, contributing to the transition from endo- to ecodormancy in apple buds. Abstract The fnal step of cytokinin (CK) signaling pathway culminates in the activation of type-B response regulators (BRRs), important transcriptional factors in the modulation of CK-responsive genes. In this study, we performed a genomewide analysis aiming to identify apple BRR family members and understand their involvement in bud dormancy control. The investigation identifed ten MdoBRR protein-coding genes. A higher expression of three MdoBRR (MdoBRR1, MdoBRR9 and MdoBRR10) was observed in dormant buds in comparison to other developmental stages. Interestingly, in ecodormant buds these three MdoBRR genes were upregulated in a CK-dependent manner. Transcription profles, determined during dormancy cycle under feld and artifcially controlled conditions, revealed that MdoBRR1 and MdoBRR8 played important roles in the transition from endo- to ecodormancy, probably mediated by endogenous CK stimuli. The expression of MdoBRR7, MdoBRR9, and MdoBRR10 was induced in ecodormant buds exposed to warm temperatures, indicating a putative role in growth resumption after chilling requirement fulfllment. Contrasting expression patternsin vivo between MdoBRRs and MdoDAM1, an essential dormancy establishment regulator, were observed during dormancy cycle and in CK-treated buds. Thereafter, in vivo transa... Mostrar Tudo |
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Palavras-Chave: |
Bud dormancy; Cytokinin; MdoBRR; MdoDAM1. |
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Thesaurus Nal: |
Apples. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/218972/1/10.1007-s00299-020-02595-z.pdf
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Marc: |
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024 7 $a10.1007_s00299-020-02595-z.pdf$2DOI
100 1 $aCATTANI, A. M.
245 $aType-B cytokinin response regulators link hormonal stimuli and molecular responses during the transition from endoto ecodormancy in apple buds.$h[electronic resource]
260 $c2020
520 $aKey Message Cytokinin together with MdoBRR1, MdoBRR8 and MdoBRR10 genes participate in the downregulation of MdoDAM1, contributing to the transition from endo- to ecodormancy in apple buds. Abstract The fnal step of cytokinin (CK) signaling pathway culminates in the activation of type-B response regulators (BRRs), important transcriptional factors in the modulation of CK-responsive genes. In this study, we performed a genomewide analysis aiming to identify apple BRR family members and understand their involvement in bud dormancy control. The investigation identifed ten MdoBRR protein-coding genes. A higher expression of three MdoBRR (MdoBRR1, MdoBRR9 and MdoBRR10) was observed in dormant buds in comparison to other developmental stages. Interestingly, in ecodormant buds these three MdoBRR genes were upregulated in a CK-dependent manner. Transcription profles, determined during dormancy cycle under feld and artifcially controlled conditions, revealed that MdoBRR1 and MdoBRR8 played important roles in the transition from endo- to ecodormancy, probably mediated by endogenous CK stimuli. The expression of MdoBRR7, MdoBRR9, and MdoBRR10 was induced in ecodormant buds exposed to warm temperatures, indicating a putative role in growth resumption after chilling requirement fulfllment. Contrasting expression patternsin vivo between MdoBRRs and MdoDAM1, an essential dormancy establishment regulator, were observed during dormancy cycle and in CK-treated buds. Thereafter, in vivo transactivation assays showed that CK stimuli combined with transient overexpression of MdoBRR1, MdoBRR8, and MdoBRR10 resulted in downregulation of the reporter gene gusA driven by the MdoDAM1 promoter. These pieces of evidences point to the integration of CK-triggered responses through MdoBRRs that are able to downregulate MdoDAM1, contributing to dormancy release in apple. Keywords MdoBRR · Cytokinin · Bud dormancy · MdoDAM1 · Apple
650 $aApples
653 $aBud dormancy
653 $aCytokinin
653 $aMdoBRR
653 $aMdoDAM1
700 1 $aFALAVIGNA, V. da S.
700 1 $aSILVEIRA, C. P.
700 1 $aBUFFON, V.
700 1 $aMARASCHIN, F. dos S.
700 1 $aPASQUALI, G.
700 1 $aREVERS, L. F.
773 $tPlant Cell Reports$gv. 39, p. 1687-1703, 2020.
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Embrapa Uva e Vinho (CNPUV) |
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| 1. |  | CATTANI, A. M.; FALAVIGNA, V. da S.; SILVEIRA, C. P.; BUFFON, V.; MARASCHIN, F. dos S.; PASQUALI, G.; REVERS, L. F. Type-B cytokinin response regulators link hormonal stimuli and molecular responses during the transition from endoto ecodormancy in apple buds. Plant Cell Reports, v. 39, p. 1687-1703, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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