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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
18/11/2020 |
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Data da última atualização: |
23/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DINATO, N. B.; SANTOS, I. R. I.; VIGNA, B. B. Z.; PAULA, A. F. de; FAVERO, A. P. |
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Afiliação: |
Naiana Barbosa Dinato, UFSCAR; IZULME RITA IMACULADA SANTOS, Cenargen; BIANCA BACCILI ZANOTTO VIGNA, CPPSE; Ailton Ferreira de Paula, UFSCAR; ALESSANDRA PEREIRA FAVERO, CPPSE. |
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Título: |
Pollen cryopreservation for plant breeding and genetic resources conservation. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Cryoletters, v. 41, n. 3, p. 115-127, may 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
Pollen conservation is an important tool for the maintenance of plant genetic resources and can promote improved efficiency in breeding programs and germplasm conservation and exchange. This review aims to highlight the importance of pollen cryopreservation and how to use it for distinct species in order to encourage the use of this methodology in germplasm banks and plant breeding programs. Pollen from many plant species have already been successfully cryopreserved in liquid nitrogen. Analogous with other plant structures, to maintain pollen viability after storage at ultra-low temperatures it is necessary to adjust the water content so that at least the freezable is removed. Optimum pollen moisture levels for cryopreservation varies among species and different methods have been applied to control moisture content. Common methods to decrease pollen moisture content include exposure to saturated solutions of various salts (which have a well-defined relative humidity), silica gel, dry air or treatment with vitrification solutions. It is our understanding that pollen cryopreservation is a safe and practical alternative for conserving genetic material that is often neglected by potential users. The technique has the potential to overcome challenges of breeding programs, such as flowering asynchrony between different parent genotypes, and the production of insufficient pollen in nature. Generally, pollen cryopreservation techniques tend to be simple enough to be used routinely in research, plant breeding and germplasm conservation programs. MenosPollen conservation is an important tool for the maintenance of plant genetic resources and can promote improved efficiency in breeding programs and germplasm conservation and exchange. This review aims to highlight the importance of pollen cryopreservation and how to use it for distinct species in order to encourage the use of this methodology in germplasm banks and plant breeding programs. Pollen from many plant species have already been successfully cryopreserved in liquid nitrogen. Analogous with other plant structures, to maintain pollen viability after storage at ultra-low temperatures it is necessary to adjust the water content so that at least the freezable is removed. Optimum pollen moisture levels for cryopreservation varies among species and different methods have been applied to control moisture content. Common methods to decrease pollen moisture content include exposure to saturated solutions of various salts (which have a well-defined relative humidity), silica gel, dry air or treatment with vitrification solutions. It is our understanding that pollen cryopreservation is a safe and practical alternative for conserving genetic material that is often neglected by potential users. The technique has the potential to overcome challenges of breeding programs, such as flowering asynchrony between different parent genotypes, and the production of insufficient pollen in nature. Generally, pollen cryopreservation techniques tend to be simple enough to be used routinely i... Mostrar Tudo |
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Palavras-Chave: |
Liquid nitrogen; Plant cryopreservation; Pollen grain. |
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Thesaurus Nal: |
Germplasm conservation; Hybridization. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/217991/1/s1.pdf
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Marc: |
LEADER 02230naa a2200229 a 4500
001 2126790
005 2020-11-23
008 2020 bl uuuu u00u1 u #d
100 1 $aDINATO, N. B.
245 $aPollen cryopreservation for plant breeding and genetic resources conservation.$h[electronic resource]
260 $c2020
520 $aPollen conservation is an important tool for the maintenance of plant genetic resources and can promote improved efficiency in breeding programs and germplasm conservation and exchange. This review aims to highlight the importance of pollen cryopreservation and how to use it for distinct species in order to encourage the use of this methodology in germplasm banks and plant breeding programs. Pollen from many plant species have already been successfully cryopreserved in liquid nitrogen. Analogous with other plant structures, to maintain pollen viability after storage at ultra-low temperatures it is necessary to adjust the water content so that at least the freezable is removed. Optimum pollen moisture levels for cryopreservation varies among species and different methods have been applied to control moisture content. Common methods to decrease pollen moisture content include exposure to saturated solutions of various salts (which have a well-defined relative humidity), silica gel, dry air or treatment with vitrification solutions. It is our understanding that pollen cryopreservation is a safe and practical alternative for conserving genetic material that is often neglected by potential users. The technique has the potential to overcome challenges of breeding programs, such as flowering asynchrony between different parent genotypes, and the production of insufficient pollen in nature. Generally, pollen cryopreservation techniques tend to be simple enough to be used routinely in research, plant breeding and germplasm conservation programs.
650 $aGermplasm conservation
650 $aHybridization
653 $aLiquid nitrogen
653 $aPlant cryopreservation
653 $aPollen grain
700 1 $aSANTOS, I. R. I.
700 1 $aVIGNA, B. B. Z.
700 1 $aPAULA, A. F. de
700 1 $aFAVERO, A. P.
773 $tCryoletters$gv. 41, n. 3, p. 115-127, may 2020.
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Embrapa Pecuária Sudeste (CPPSE) |
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