| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
25/08/2020 |
|
Data da última atualização: |
25/08/2020 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
LEASTRO, M. O.; CASTRO, D. Y. O.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á. |
|
Afiliação: |
MIKHAIL OLIVEIRA LEASTRO, Instituto Biológico; DEIBIS YORLENIS ORTEGA CASTRO, Universidad Politécnica de Valencia; JULIANA DE FREITAS ASTUA, CNPMF; ELLIOT WATANABE KITAJIMA, ESALQ; VICENTE PALLÁS, Universidad Politécnica de Valencia; JESÚS ÁNGEL SÁNCHEZ-NAVARRO, Universidad Politécnica de Valencia. |
|
Título: |
Citrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity. |
|
Ano de publicação: |
2020 |
|
Fonte/Imprenta: |
Frontiers in Microbiology, June 2020. |
|
ISSN: |
1664-302X |
|
DOI: |
https://doi.org/10.3389/fmicb.2020.01231 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Citrus leprosis virus C (CiLV-C) belongs to the genus Cilevirus, family Kitaviridae, and is considered the most devastating virus infecting citrus in Brazil, being the main viral pathogen responsible for citrus leprosis (CL), a severe disease that affects citrus orchards in Latin America. Here, proteins encoded by CiLV-C genomic RNA 1 and 2 were screened for potential RNA silencing suppressor (RSS) activity by five methods. Using the GFP-based reporter agroinfiltration assay, we have not found potential local suppressor activity for the five CiLV-C encoded proteins. However, when RSS activity was evaluated using the alfalfa mosaic virus (AMV) system, we found that the p29, p15, and p61 CiLV-C proteins triggered necrosis response and increased the AMV RNA 3 accumulation, suggesting a suppressive functionality. From the analysis of small interfering RNAs (siRNAs) accumulation, we observed that the ectopic expression of the p29, p15, and p61 reduced significantly the accumulation of GFP derived siRNAs. The use of the RSS defective turnip crinkle virus (TCV) system revealed that only the trans-expression of the p15 protein restored the cell-to-cell viral movement. Finally, the potato virus X (PVX) system revealed that the expression of p29, p15, and p61 increased the PVX RNA accumulation; in addition, the p29 and p15 enhanced the pathogenicity of PVX resulting in the death of tobacco plants. Furthermore, PVX-p61 infection resulted in a hypersensitive response (HR), suggesting that p61 could also activate a plant defense response mechanism. This is the first report describing the RSS activity for CiLV-C proteins and, moreover, for a member of the family Kitaviridae. MenosCitrus leprosis virus C (CiLV-C) belongs to the genus Cilevirus, family Kitaviridae, and is considered the most devastating virus infecting citrus in Brazil, being the main viral pathogen responsible for citrus leprosis (CL), a severe disease that affects citrus orchards in Latin America. Here, proteins encoded by CiLV-C genomic RNA 1 and 2 were screened for potential RNA silencing suppressor (RSS) activity by five methods. Using the GFP-based reporter agroinfiltration assay, we have not found potential local suppressor activity for the five CiLV-C encoded proteins. However, when RSS activity was evaluated using the alfalfa mosaic virus (AMV) system, we found that the p29, p15, and p61 CiLV-C proteins triggered necrosis response and increased the AMV RNA 3 accumulation, suggesting a suppressive functionality. From the analysis of small interfering RNAs (siRNAs) accumulation, we observed that the ectopic expression of the p29, p15, and p61 reduced significantly the accumulation of GFP derived siRNAs. The use of the RSS defective turnip crinkle virus (TCV) system revealed that only the trans-expression of the p15 protein restored the cell-to-cell viral movement. Finally, the potato virus X (PVX) system revealed that the expression of p29, p15, and p61 increased the PVX RNA accumulation; in addition, the p29 and p15 enhanced the pathogenicity of PVX resulting in the death of tobacco plants. Furthermore, PVX-p61 infection resulted in a hypersensitive response (HR), suggesting th... Mostrar Tudo |
|
Thesagro: |
Doença de Planta; Fruta Cítrica. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02381naa a2200229 a 4500
001 2124547
005 2020-08-25
008 2020 bl uuuu u00u1 u #d
022 $a1664-302X
024 7 $ahttps://doi.org/10.3389/fmicb.2020.01231$2DOI
100 1 $aLEASTRO, M. O.
245 $aCitrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity.$h[electronic resource]
260 $c2020
520 $aCitrus leprosis virus C (CiLV-C) belongs to the genus Cilevirus, family Kitaviridae, and is considered the most devastating virus infecting citrus in Brazil, being the main viral pathogen responsible for citrus leprosis (CL), a severe disease that affects citrus orchards in Latin America. Here, proteins encoded by CiLV-C genomic RNA 1 and 2 were screened for potential RNA silencing suppressor (RSS) activity by five methods. Using the GFP-based reporter agroinfiltration assay, we have not found potential local suppressor activity for the five CiLV-C encoded proteins. However, when RSS activity was evaluated using the alfalfa mosaic virus (AMV) system, we found that the p29, p15, and p61 CiLV-C proteins triggered necrosis response and increased the AMV RNA 3 accumulation, suggesting a suppressive functionality. From the analysis of small interfering RNAs (siRNAs) accumulation, we observed that the ectopic expression of the p29, p15, and p61 reduced significantly the accumulation of GFP derived siRNAs. The use of the RSS defective turnip crinkle virus (TCV) system revealed that only the trans-expression of the p15 protein restored the cell-to-cell viral movement. Finally, the potato virus X (PVX) system revealed that the expression of p29, p15, and p61 increased the PVX RNA accumulation; in addition, the p29 and p15 enhanced the pathogenicity of PVX resulting in the death of tobacco plants. Furthermore, PVX-p61 infection resulted in a hypersensitive response (HR), suggesting that p61 could also activate a plant defense response mechanism. This is the first report describing the RSS activity for CiLV-C proteins and, moreover, for a member of the family Kitaviridae.
650 $aDoença de Planta
650 $aFruta Cítrica
700 1 $aCASTRO, D. Y. O.
700 1 $aASTUA, J. de F.
700 1 $aKITAJIMA, E. W.
700 1 $aPALLÁS, V.
700 1 $aSÁNCHEZ-NAVARRO, J. Á.
773 $tFrontiers in Microbiology, June 2020.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Gado de Corte. |
|
Data corrente: |
01/08/2005 |
|
Data da última atualização: |
27/07/2009 |
|
Autoria: |
AMARAL, T. B. |
|
Afiliação: |
Embrapa Gado de Corte (Campo Grande, MS). |
|
Título: |
Técnicas de reprodução e sua aplicação na disseminação do ganho genético. |
|
Ano de publicação: |
2005 |
|
Fonte/Imprenta: |
In: SIMPÓSIO DE PECUÁRIA DE CORTE, 4., 2005, Lavras. Otimizando a pecuária de corte. Lavras: UFLA, 2005. |
|
Páginas: |
p. 235-261. |
|
Idioma: |
Português |
|
Notas: |
CNPGC. |
|
Palavras-Chave: |
Fecundação in vitro; Sexagem. |
|
Thesagro: |
Acasalamento; Biotecnologia; Inseminação Artificial; Reprodução Animal; Tecnologia; Transferência de Embrião. |
|
Thesaurus NAL: |
animal reproduction; artificial insemination; biotechnology; copulation; embryo transfer; in vitro fertilization; sex determination; technology. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 00979naa a2200325 a 4500
001 1325807
005 2009-07-27
008 2005 bl uuuu u00u1 u #d
100 1 $aAMARAL, T. B.
245 $aTécnicas de reprodução e sua aplicação na disseminação do ganho genético.
260 $c2005
300 $ap. 235-261.
500 $aCNPGC.
650 $aanimal reproduction
650 $aartificial insemination
650 $abiotechnology
650 $acopulation
650 $aembryo transfer
650 $ain vitro fertilization
650 $asex determination
650 $atechnology
650 $aAcasalamento
650 $aBiotecnologia
650 $aInseminação Artificial
650 $aReprodução Animal
650 $aTecnologia
650 $aTransferência de Embrião
653 $aFecundação in vitro
653 $aSexagem
773 $tIn: SIMPÓSIO DE PECUÁRIA DE CORTE, 4., 2005, Lavras. Otimizando a pecuária de corte. Lavras: UFLA, 2005.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Corte (CNPGC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
