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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
25/06/2020 |
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Data da última atualização: |
25/06/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ALVIM, B. F. M.; SOUZA, A. V. de; BRITO, A. L.; FONSECA, P. T.; SOARES, T. L; SANTANA, J. R. F. de. |
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Afiliação: |
Bruno Freitas Matos Alvim; ANA VALERIA VIEIRA DE SOUZA, CPATSA; Alone Lima Brito; Priscila Tavares Fonseca; Taliane Leila Soares; José Raniere Ferreira de Santana. |
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Título: |
In vitro conservation of Amburana cearensis (Fabaceae). |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Ciência Rural, v. 50, n. 7, e20190729, 2020. |
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ISSN: |
1678-4596 |
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DOI: |
http://doi.org/10.1590/0103-8478cr20190729 |
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Idioma: |
Inglês |
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Conteúdo: |
Slow-growth tissue culture allows in vitro conservation of plant genetic resources and is a complementary technique to conventional preservation methods. The objective of this study was to evaluate the effect of the culture medium supplemented with sucrose, mannitol and sorbitol osmotic agents on the induction of in vitro slow growth of A. cearensis, seeking to establish alternative techniques for ex situ conservation of the species. Plants with age of 10 days were inoculated in woody plant medium (WPM) supplemented with different concentrations of sucrose (87.64, 131.46, 175.28 or 262.92 mM) combined with mannitol or sorbitol at varying concentrations (0.0, 43.8, 65.73, 87.64 or 131.46 mM), solidified with 0.7% agar. The following parameters were evaluated: survival percentage, number of senescent leaves, aerial part length, root length, aerial part dry mass, root dry mass; and number of shoots. The conservation of A. cearensis was viable for up to 300 days in WPM supplemented with 262.92 mM of sucrose or the combination of 87.64 mM of sucrose with 43.80 mM of mannitol, because besides achieving a survival percentage greater than 55.0%, the plants conserved in these media presented normal development and the best appearances, with predominance of uniform green leaves during the storage process. |
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Palavras-Chave: |
Agente osmótico; Amburana cearensis; Conservação de germoplasma; Crescimento mínimo; Planta nativa; Retardante de crescimento; Umburana-de- cheiro. |
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Thesagro: |
Caatinga; Cultura de Tecido; Cumaru; Planta Medicinal; Recurso Genético; Vegetação Nativa. |
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Thesaurus Nal: |
Medicinal plants; Tissue culture; Vegetation. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/214216/1/In-vitro-conservation-of-Amburana-cearensis-2020.pdf
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Marc: |
LEADER 02445naa a2200397 a 4500
001 2123464
005 2020-06-25
008 2020 bl uuuu u00u1 u #d
022 $a1678-4596
024 7 $ahttp://doi.org/10.1590/0103-8478cr20190729$2DOI
100 1 $aALVIM, B. F. M.
245 $aIn vitro conservation of Amburana cearensis (Fabaceae).$h[electronic resource]
260 $c2020
520 $aSlow-growth tissue culture allows in vitro conservation of plant genetic resources and is a complementary technique to conventional preservation methods. The objective of this study was to evaluate the effect of the culture medium supplemented with sucrose, mannitol and sorbitol osmotic agents on the induction of in vitro slow growth of A. cearensis, seeking to establish alternative techniques for ex situ conservation of the species. Plants with age of 10 days were inoculated in woody plant medium (WPM) supplemented with different concentrations of sucrose (87.64, 131.46, 175.28 or 262.92 mM) combined with mannitol or sorbitol at varying concentrations (0.0, 43.8, 65.73, 87.64 or 131.46 mM), solidified with 0.7% agar. The following parameters were evaluated: survival percentage, number of senescent leaves, aerial part length, root length, aerial part dry mass, root dry mass; and number of shoots. The conservation of A. cearensis was viable for up to 300 days in WPM supplemented with 262.92 mM of sucrose or the combination of 87.64 mM of sucrose with 43.80 mM of mannitol, because besides achieving a survival percentage greater than 55.0%, the plants conserved in these media presented normal development and the best appearances, with predominance of uniform green leaves during the storage process.
650 $aMedicinal plants
650 $aTissue culture
650 $aVegetation
650 $aCaatinga
650 $aCultura de Tecido
650 $aCumaru
650 $aPlanta Medicinal
650 $aRecurso Genético
650 $aVegetação Nativa
653 $aAgente osmótico
653 $aAmburana cearensis
653 $aConservação de germoplasma
653 $aCrescimento mínimo
653 $aPlanta nativa
653 $aRetardante de crescimento
653 $aUmburana-de- cheiro
700 1 $aSOUZA, A. V. de
700 1 $aBRITO, A. L.
700 1 $aFONSECA, P. T.
700 1 $aSOARES, T. L
700 1 $aSANTANA, J. R. F. de
773 $tCiência Rural$gv. 50, n. 7, e20190729, 2020.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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| 1. |  | ALVIM, B. F. M.; SOUZA, A. V. de; BRITO, A. L.; FONSECA, P. T.; SOARES, T. L; SANTANA, J. R. F. de. In vitro conservation of Amburana cearensis (Fabaceae). Ciência Rural, v. 50, n. 7, e20190729, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Semiárido. |
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| Registros recuperados : 1 | |
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